南瓜组培根根系分泌物的化感效应研究

摘要/Abstract

摘要： 使用组织培养技术、生物测试法及室内分析相结合的方法研究了南瓜组培根在不同生长时期与不同营养谐迫下(利用正交设计调节标准B₅培养基中的大量元素、微量元素、有机质和激素)根分泌物对南瓜、萝卜和小麦3种受体的种子萌发及幼苗生长的化感作用.结果表明,南瓜具有自毒作用,南瓜组培根根系分泌物对受体幼苗生长的抑制作用呈现出高抑低促的作用表型;南瓜组培根根系分泌物产生化感作用的活性与南瓜组培根生长速度有关,南瓜组培根根系分泌物的生长抑制活性以15～17d即生长速度指数末期为最高,21d生物量最大时的南瓜组培根根系分泌物的生长抑制活性最低;营养元素的改变明显影响了南瓜根系分泌物的产生,并通过筛选出能诱导南瓜组培根对受体具有强烈抑制作用的大量元素、微量元素、维生素和激素种类和含量的最优化组合,为研究南瓜化感作用营养胁迫机理提供依据.

关键词: 南瓜, 根系分泌物, 化感作用, 组培, 正交技术

Abstract: By using the techniques of tissue culture,bio-assay and laboratory analysis,this paper studied the effects of the allelopathic chemicals from pumpkin (Cucurbita moschata) roots on the seed germination and seedling growth of pumpkin,wheat (Triticum aestivum),and radish (Raphanus sativus).The pumpkin root was cultured on a sterile B₅ media,and the concentrations of macro- and microelements,organic supplements and hormones in the media were adjusted by using an orthogonal design.After culturing,the culture media was filtered and used in a bioassay to test the autotoxicity and allelopathic effects.The results showed that the pumpkin had both autotoxic and allelopathic effects,and the media having been used to culture the pumpkin roots contained the chemicals that significantly inhibited the seedling growth of wheat and radish.The allelopathic effect decreased when the culture media was diluted.The production of allelochemicals seemed to be related to the growth rate of the pumpkin roots.When the root growth was rapid,the concentration of allelochemicals was high.The allelopathic effect was stronger on radish than on wheat.The optimum concentrations of macro- and microelements,vitamins and hormones for culturing pumpkin root were determined,and the effect of pumpkin root nutrition on the production of allelochemicals was tested.The results indicated that pumpkin root nutrition had a significant effect on the production of allelochemicals.

Key words: Cucurbita moschata, Root exudates, Allelopathy, Orthogonal design

中图分类号:

Q948.12⁺2.1

李明, 马永清, 税军峰. 南瓜组培根根系分泌物的化感效应研究[J]. 应用生态学报, 2005, 16(4): 744-749.

LI Ming, MA Yongqing, SHUI Junfeng. Allelopathic effects of cultured Cucurbita moschata root exudates[J]. Chinese Journal of Applied Ecology, 2005, 16(4): 744-749.

参考文献

[1] Anaya AL. 1999. Alleolpathy as a tool in the management of biotic resources in agroecosystems. Crit Rev Plant Sci, 18(6):697～739
[2] Ben-Hammouda M, Ghorbal H, Kremer RJ. 2002. Autotoxicity ofbarley. J Plant Nutr, 25(6):1155～1161
[3] Chen L-C(陈龙池),Liao L-P(廖利平),Wang S-L(汪思龙),et al.2002.A review for research of root exudates ecology.Chin J Ecol(生态学杂志),21(6):57～62(in Chinese)
[4] Chon Sang UK, Coutts JH, Nelson CJ, et al. 2000. Effects of light,growth media, and seedling orientation on bioassays of alfalfa autotoxicity. Agron J, 92(4):715～720
[5] Daizy RB, Singh HP, Kohli RK, et al. 2001. Crop allelopathy and its role in ecological agriculture. In:Ravinder K ed. Allelopathy in Agroecosystems. India:The Haworth Press. 121～161
[6] Fageria NK, Baligar VC. 2003. Upland rice and allelopathy. CommSoil Sci Plant Anal, 34(9～10):1311～1329
[7] Gao M-X(高梅秀),Li S-H(李树和),Liu Y-Q(刘玉芹),et al.2001.Effect of different stocks on resistance,physical activity and output of eggplant.Acta Horticul Sin(园艺学报),28(5):463～465(in Chinese)
[8] Gao Z-Q(高子勤),Zhang S-X(张淑香).1998.Continuous cropping obstacle and rhizospheric microecology I.Root exudates and their ecological effects.Chin J Appl Ecol(应用生态学报),9(5):549～554(in Chinese)
[9] Han L-M(韩丽梅),Wang S-Q(王树起),Ju H-Y(鞠会艳),et al.2000.Identifition and study on allelopathy of soybean root exudates.Soybean Sci(大豆科学),19(2):119～125(in Chinese)
[10] Hu F(胡飞),Kong C-H(孔垂华).2002.Allelopathic potentials of Arachys hypogaea on crops.J South China Agric Univ(华南农业大学学报),23(1):9～12(in Chinese)
[11] 0Kaushal R,Verna KS,Singh KN.2003.Effect of Grewia optiva and Populus deltoides leachates on field crops.Alleolopathy J,11:229～234
[12] Kong C-H(孔垂华),HU F(胡飞),Chen X-H(陈雄辉),et al.2002.Assessment and utilization of a11elopathic crop varietal resources.Sci Agric Sin(中国农业科学),35(9):1159～1164(in Chinese)
[13] Leather GR,Einhe11ing FA.1986.Bioassays in the study of allelopathy.In:Putnam AR,Tang CS,eds.The Science of Allelopathy.New York:John Wiley＆Sons.133～145
[14] Lu W-G(吕卫光),Zhang C-L(张春兰),Yuan F(袁飞),et al.2002.Mechanism of organic manure relieving the autotoxicity to continuous cropping cucumber.Acta Agric Shanghai(上海农业科学),18(2)52～56(in Chinese)
[15] Mou Z-P(牟子平),Lei H-M(雷红梅).1999.Allelopathy in the cropintercropping.J Hunan Agric Univ(湖南农业大学学报),25(3):262～266(in Chinese)
[16] Pramanik MHR, Asao T, Yamamoto T, et al. 2001. Sensitive bioassay to evaluate toxicity of aromatic acids to cucumber seedlings. AllelopathyJ, 18(2):161～169
[17] Pramanik MHR. 2000. Effects of temperature and photoperiod on phytotoxic root exudates of cucumber in hydroponic culture. JChem Ecol, 26(8):1953～1967
[18] Rice EL. 1984. Allelopathy. New York:Academy Press. 151～187
[19] Singh HP, Kohli RK, Batish DR. 1999. Autotoxicity:Concept, organisms, and ecological significance. Crit Rev Plant Sci, 18 (6):757～772
[20] Sun Y(孙艳),Huang W(黄炜),Tian X-H(田霄鸿).2002.Study on growth situation, photosynthetic characteristics and nutrient absorption characteristics of grafted cucumber seedlings. Plant Nutrit Fert Sci(植物营养与肥料学报),8(2):181～185(in Chinese)
[21] Wang TSC, Kao MM, Li SW. 1984. The exploration and improvement of the yield decline of monoculture sugarcane in Taiwan. In:Chou CH ed. Tropical Plant. Monograph. No. 5. Taipei, Taiwan:Institute of Botany, Academia Sinica. 1～9
[22] Williamson GB, et al. 1988. Bioassay for allelopathy:Measuring treatment responses with independent controls. J Chem Ecol, 14(1):181～187
[23] Xing G-M(邢国明),Kang X-P(亢秀萍),Ji Q-Y(姬青云),et al.2000.Advances in the research of cultivation of grafting aubergine and fruit vegetable.J Shenyang Agric Univ(沈阳农业大学学报),31(1):144～146(in Chinese)
[24] Yu J-Q(喻景权),Du Y-S(杜尧舜).2000.Soil-sickness problem in the sustainable development for the protected production of vegetables.J Shenyang Agric Univ(沈阳农业大学学报),31(1):124～126(in Chinese)
[25] Yu J-Q(喻景权),Matsui Yoshihisa.1999.Autointoxication of root exudatesin Pisum sativus.Acta Hortic Sin(园艺学报),26(3):175～179(in Chinese)
[26] Yu JQ, Matsui Yoshihisa. 1994. Phytotoxic substances in root exudates of cucumber. J Chem Ecol, 20 (1):21～30
[27] Yu JQ, Shou SY, Qian YR, et al. 2000. Autotoxic potential of cucurbit crops. Plant and Soil, 223 (1):147～151
[28] Yu JQ. 1999. Autotoxic potential of vegetable crops. In:Narualss,ed. Allelopathy Update-Basic and Applied Aspects. NH, USA:Science Publishers Inc. 159～162
[29] Zeng R-S(曾任森).1999.Review on bioassay methods for allelopathy research.Chin J Appl Ecol(应用生态学报),10(1):123～126(in Chinese)
[30] Zhou B-L(周宝利),Jiang H(姜荷).2001.Relation betweencharacteristics of resistance to Verticillium wilt of eggplant by graftage and root exudates of eggplant.J Shenyang Agric Univ(沈阳农业大学学报),32(6):414～417(in Chinese)
[31] Zhou Z-H(周志红),Luo S-M(骆世明),Mou Z-P(牟子平).1997.Allelopathic effect oftomato.Chin J Appl Ecol(应用生态学报),8(4):445～449(inChinese)
[32] Zhou Z-H(周志红).1999.Methods of allelopahty bioassay and the affecting factors.Ecol Sci(生态科学),18(1):35～38(in Chinese)
[33] Zhu L-X(朱丽霞),Zhang J-E(章家恩),Liu W-G(刘文高).2003.Review of studies on interactions between root exudates and rhizospheric microorganisms.Ecol Environ(生态环境),12(1):102～105(in Chinese)