培养条件对海洋假单胞菌脂肪酸的影响

应用生态学报 ›› 2005, Vol. 16 ›› Issue (5): 967-970.

培养条件对海洋假单胞菌脂肪酸的影响

叶芳挺¹, 严小军¹, 郑立^2,3, 徐继林¹, 陈海敏¹

1. 宁波大学生命科学与生物工程学院, 宁波 315211;
2. 中国科学院海洋研究所, 青岛 266071;
3. 国家海洋局第一海洋研究所, 青岛 266061

收稿日期:2004-04-14 修回日期:2004-08-02 出版日期:2005-05-15 发布日期:2005-05-15
通讯作者: 严小军,E-mail:xiaojunyan@hotmail.com
基金资助:
国家自然科学基金重点项目(29932030)和国家科技部新药博士基金资助项目(2003AA2Z3511).

Effects of culture condition on fatty acids in Pseudomonas sp

1. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China;
2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
3. First Institute Oceanography of SOA, Qingdao 266061, China

Received:2004-04-14 Revised:2004-08-02 Online:2005-05-15 Published:2005-05-15

摘要/Abstract

摘要： 研究了不同温度条件下一株海绵附生假单胞菌(Pseudomonassp.)在不同碳源培养基中的生长情况及脂肪酸变化.结果表明,该海洋菌生长最适温度为30℃,在以淀粉作为外加碳源的培养基中生长最好;实验菌含13种脂肪酸,主要是c16:1(n7)、c15:0、c16:0、c17:0、c18:1(n6)、c18:1(n9)、9,10cp c17:0和其同分异构体.在30℃温度条件下,不饱和脂肪酸的相对含量急剧减少.在有外加碳源(葡萄糖和淀粉)的培养基中生长的细菌,奇数脂肪酸和环丙基脂肪酸含量远比未外加碳源的低.聚类分析结果表明,两种环境因子中,温度比碳源的影响更明显.

关键词: 海洋假单胞菌, 培养条件, 脂肪酸

Abstract: The investigation on the growth of Pseudomonas sp.and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 ℃,and the best carbon source was starch.13 kinds of fatty acids were detected,among which,c16:1(n-7),c15:0,c16:0,c17:0,c18:1(n-6),c18:1(n-9),9,10-cp-c17:0 and iso9,10-cp-c17:0 were the major ones.When cultured at 30 ℃,the relative content of unsaturated fatty acids decreased significantly.Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids.Cluster analysis revealed that temperature had a more obvious effect than carbon source.

Key words: Marine bacterium (Pseudomonas sp), Culture condition, Fatty acids

中图分类号:

Q936

叶芳挺, 严小军, 郑立, 徐继林, 陈海敏. 培养条件对海洋假单胞菌脂肪酸的影响[J]. 应用生态学报, 2005, 16(5): 967-970.

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