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应用生态学报 ›› 2005, Vol. 16 ›› Issue (6): 1038-1042.

• 研究论文 • 上一篇    下一篇

二氧化硫对小麦的氧化胁迫及其某些信号分子的调节

郝林1, 张惠文2, 徐昕1, 陶思源1, 于龙1   

  1. 1. 沈阳师范大学生物系, 沈阳 110034;
    2. 中国科学院沈阳应用生态研究所, 沈阳 110016
  • 收稿日期:2004-10-12 修回日期:2005-02-28 出版日期:2005-06-15 发布日期:2005-06-15
  • 通讯作者: 郝林,男,1963年出生,博士,教授.主要从事植物对生物和非生物胁迫应答的研究,发表论文40多篇.E-mail:haolinwj2001@yahoo.com.cn
  • 基金资助:
    国家自然科学基金项目(30070129)和辽宁省博士启动基金项目(023804111)和沈阳师范大学实验中心主任基金资助项目(SY200406).

SO2-caused oxidative stress and modulation of some signal molecules in wheat

HAO Lin1, ZHANG Huiwen2, XU Xin1, TAO Siyuan1, YU Long 1   

  1. 1. Department of Biology, Shenyang Normal University, Shenyang 110034, China;
    2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2004-10-12 Revised:2005-02-28 Online:2005-06-15 Published:2005-06-15

摘要: 通过在密闭的培养箱中一次性通入不同体积浓度的SO2气体,研究了小麦幼苗超氧自由基O2含量和3种抗氧化酶活性的变化,探讨了信号分子水杨酸、乙烯和过氧化氢对SO2氧化胁迫的调节作用.结果表明,当通入10和40μl·L-1SO2时,小麦叶片中O2含量递增,过氧化物酶(POD)和过氧化氢酶(CAT)活性增强,但超氧化物歧化酶(SOD)活性降低.当SO2浓度达到50μl·L-1时,POD和CAT活性也开始降低,此时叶片尖端出现坏死,叶片绿色部位滋生大量真菌.用1mmol·L-1水杨酸(SA)(pH6.5)浸泡小麦干种子6h,或用10mmol·L-1H2O2浸泡幼苗,O2含量低于对照植株,而3种酶的活性高于对照植株,均可有效地减轻SO2的氧化胁迫.在SO2熏蒸下,乙烯显著抑制3种酶的活力,提高O2的形成速率.SA与乙烯同时使用时,SA几乎完全消除了乙烯的负面作用.

关键词: 二氧化硫, 小麦, 水杨酸, 乙烯, 过氧化氢

Abstract: By adding different concentration SO2 to culture boxes,this paper studied the responses of wheat seedling's superoxide anion radical O2 and antioxidant enzymes to SO2.The results showed that when the concentration of SO2 was 10 and 40 μl·L-1,the O2 content and peroxidase (POD) and catalase(CAT) activities of wheat leaf were increased,while the activity of superoxide dismutase(SOD) was reduced.When the SO2 concentration was 50 μl·L-1,the activities of POD and CAT were decreased,with the top end leaves appeared necrosis,and more fungi multiplied on green leaves.Presoaking wheat seeds with 1 mmol·L-1 salicylic acid (SA) for 6 h or with 10 mmol·L-1 H2O2 for 12 h could efficiently alleviate the oxidative stress caused by SO2,with the O2 content decreased and the activities of test antioxidant enzymes increased.Under SO2 fumigation,ethylene significantly inhibited the activities of test enzymes,and promoted the O2 production rate.When applying SA and ethylene at the same time,SA could almost completely eliminate the influences of ethylene on O2 production and test enzyme activities.

Key words: SO2, Wheat, Salicylic acid, Ethylene, Hydrogen peroxide

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