尖角突脐孢菌与化学除草剂混用防治稻田稗草效果的研究

应用生态学报 ›› 2005, Vol. 16 ›› Issue (6): 1104-1107.

尖角突脐孢菌与化学除草剂混用防治稻田稗草效果的研究

陈勇^1,2, 倪汉文², 张宏军³, 李晓晶², 李健强⁴

1. 华南农业大学农学院, 广州 510642;
2. 中国农业大学农学与生物技术学院, 北京 100094;
3. 农业部农药检定所, 北京 100026;
4. 农业部农药化学与应用技术重点开放实验室, 北京 100094

收稿日期:2004-04-19 修回日期:2004-11-01 出版日期:2005-06-15 发布日期:2005-06-15
通讯作者: 倪汉文
基金资助:
国家农业部农业结构调整重大技术研究专项(2003-07-03B)和华南农业大学校长基金资助项目.

Prevention efficiency of Exserohilum monoceras with chemical herbicides against Echinochloa crus-galli in paddy field

CHEN Yong^1,2, NI Hanwen², ZHANG Hongjun³, LI Xiaojing², LI Jianqiang ⁴

1. College of Agronomy, South China Agricultural University, Guangzhou 510642, China;
2. Department of Pesticides and Plant Quarantine, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;
3. Institute for Control of Agrochemicals, Ministry of Agriculture, Beijing 100026, China;
4. Key Laboratory of Pesticide Chemical Research and Utilization, Ministry of Agriculture, Beijing 100094, China

Received:2004-04-19 Revised:2004-11-01 Online:2005-06-15 Published:2005-06-15

摘要/Abstract

摘要： 用平板表面萌发法测定化学除草剂对尖角突脐孢菌菌株X27分生孢子萌发的影响,在温室研究该菌分生孢子干粉与化学除草剂的相互作用,在田间评价其混剂的除稗效果.结果表明,二氯喹啉酸和苄嘧磺隆对分生孢子的萌发影响不明显,其它除草剂均有不同程度的影响.在温室条件下,菌X27干粉与二氯喹啉酸混用具有明显的增效作用,与敌稗混用具有加成作用;在水田条件下,单用菌X27干粉防除稗草效果差,防效只有60%,而与低量的二氯喹啉酸混用,防效明显提高,达90%以上.

关键词: 尖角突脐孢菌, 稗草, 化学除草剂, 增效作用, 水稻

Abstract: This paper studied the impact of several herbicides on the conidium germination of Exserohilum monoceras strain X27 in petri dish,and the synergistic effects of the pathogen and chemical herbicide quinclorac or propanil against Echinochloa crus-galli in greenhouse.The prevention efficiency of the tank-mixture of pathogen and quinclorac was also evaluated in paddy field.The results showed that test herbicides except quinclorac and bensulfulfuron-methyl could inhibit conidium germination and hypha growth to different degree.A significant synergism was observed between the pathogen and quinclorac.Adding quinclorac could obviously increase the prevention efficiency against Echinochloa crus-galli.Under field condition,the single use of pathogen could only control about 60% of the weed,while the efficiency of pathogen-quinclorac mixture could reach 90%.

Key words: Exserohilum monoceras, Echinochloa crus-galli, Herbicide, Synergism, Paddy field

中图分类号:

S365

陈勇, 倪汉文, 张宏军, 李晓晶, 李健强. 尖角突脐孢菌与化学除草剂混用防治稻田稗草效果的研究[J]. 应用生态学报, 2005, 16(6): 1104-1107.

CHEN Yong, NI Hanwen, ZHANG Hongjun, LI Xiaojing, LI Jianqiang . Prevention efficiency of Exserohilum monoceras with chemical herbicides against Echinochloa crus-galli in paddy field[J]. Chinese Journal of Applied Ecology, 2005, 16(6): 1104-1107.

参考文献

[1] Bailey BA, Prakash-Hebbar K, Strem M, et al. 1998. Formulations of Fusarium oxysporum f. sp. erythroxyli for biocontrol of Erythroxylum coca var. coca. Weed Sci, 46: 682～ 689
[2] ChenY(陈勇),Ni H-W(倪汉文).2003.Analysis of RAPD fingerprinting in Exserohilum monoceras strain.Acta Microbiol Sin (微生物学报),43(4):409～416(in Chinese)
[3] Chen Y(陈勇),Ni H-W(倪汉文).1999.Pathogenicity of indigenous fungi to Echinochloa crus-galli and rice(Oryza sativa).Chin J Biol Cont(中国生物防治),15(2):73～76(in Chinese)
[4] Chen Y(陈勇),Ni H-W(倪汉文).2001.Efficacy of Exserohilum monoceras in controlling of Echinochloa crus-galli in rice (Orca sattva).Acta Phytophyl Sin(植物保护学报),28(1):75～78(in Chinese)
[5] Crump NS, Cother EJ, Ash GJ. 1999. Clarifying the nomenclature in microbial weed control. Biocont Technol, 9:89～97
[6] Daigle DJ, Connick WJ, Boyette CD, et al. 1998. Solid state fermentation plus extrusion to make biopesticide granules. Biotechnol Tech, 12: 715～ 719
[7] Ekesi S, Maniana NK, Ampong-Nyarko K. 1999. Effect of temperature on germination, radial growth and virulence of Metarhizium anisopliae and Beauveria bassiana on Megalurothrips sjostedti.Biocont Tech nol, 9:177～ 185
[8] Ellis SM. 1975. Genetic variation in herbicide resistance in scentless mayweed. Weed Res, 15(3) :307～315
[9] Finney DJ. 1987. Statistical Method in Biological Assay. 3rd ed.London: Charles Griffin.
[10] Gomez KA, Gomez AA. 1984. Statistical Procedures for Agricultural Research. 2nd ed. New York:John Wiley. 680
[11] Hebbar KP, Lumsden RD, Lewis JA, et al. 1998. Formulation of mycoherbicidal strains of Fusarium oxysporum. Weed Sci, 46:501～507
[12] Hetherington SD, Tuat NV, Auld BA, et al. 2001. The development of a bioherbicde for Echinochloa crus-galli based on the fungal pathogen Exserochilum monoceras in Vietanam. In: Ni HW,You ZG, eds. The 18th Asian-Pacific Weed Science Society Conference. Beijing: China Standards Press. 377～381
[13] Holm LG, Plucknett JV, Herberger JP. 1977. The World's Worst Weeds- Distribution and Biology. Honolulu: Hawaii University Press. 32～ 46
[14] Hong TD, Jenkins NE, Ellis RH. 1999. Fluctuating temperature and longevity of conidia of metarhizium flavoviride in storage. Biocont Technol, 9:165～ 176
[15] Hu F(胡飞),Kong C-H(孔垂华).2004.Selectivity and influence of parasite plants on their hosts.Chin J Appl Ecol(应用生态学报),15(5):905～908(in Chinese)
[16] Huang B-Q(黄炳球),Xiao Z-Y(肖整玉),Lin S-X(林韶湘).1995.The present situation of resistance of the bamyardgrass to butachlorin the rice planting areas in China.Acta Phytophyl Sin (植物保护学报),22(3):281～286(in Chinese)
[17] Huang J-H(黄京华),Luo S-M(骆世明),Zeng R-S(曾任森).2003.Mechanisms of plant disease resistance by arbuscular mycorrhizal fungi.Chin J Appl Ecol(应用生态学报),14(5):819～822(in Chinese)
[18] Huang B-Q(黄炳球),LinS-X(林韶湘).1992.Studies on action of butachlor on barnyardgrass.J WeedSci(杂草学报),6(2):29～35(in Chinese)
[19] Huang B-Q(黄炳球),Xiao Z-Y(肖整玉),Lin S-X(林韶湘).1995.A present situation about the resistance ofthe barnyardgrass to benthiocarb in the paddy fields in China.J South China Agric Univ(华南农业大学学报),16(3):17～21(in Chinese)
[20] Lewis JA, Larkin RP. 1998. Formulation of the biocontrol fungus Cladorrhinum foecundissimum to reduce damping-off disease caused by Rhizoctonia solani and Pythium ultimum. Biol Cont,12:182 ～ 190
[21] Liu W-Q(刘蔚秋),Wang Y-F(王永繁),Xu R-L(徐润林).2001.Ecological characteristics of phytoplankton in waters of biologicalcontrolling and ordinary rice fields.Chin J Appl Ecol(应用生态学报), 12 (1): 59 ～ 62 (in Chinese)
[22] Lopez-martines N, Gonzalez-gutierrez J, De-Prado R. 2001. Propanil activity, uptake and metabolism in resistant Echinochloa spp. biotypes. Weed Res, 41:187～ 196
[23] Moore DJ, Langewald FO. 1997. Effects of rehydration on the conidial viability of Metarhizium flavoviride mycopesticide formulations. Biocont Sci Technol, 7: 87～ 94
[24] Norsworthy LK, Talbert RE, Hoagland RE. 1998. Chlorophyll fluorescence for rapid detection of propanil resistant bamyardgrass (Echinochloa crus-galli). Weed Sci, 46:163 ～ 169
[25] Oannis BV, Ilias GE, Kico VD. 2000. Propanil-resistant Barnyardgrass (Echinochloa crus-galli) biotypes found in Greece. Weed Technol, 14: 524～ 529
[26] Wang G-H(王光华), Jos MR. 2004. Antibiotics production by bacterial agents and its role in biological control. Chin J Appl Ecol (应用生态学报), 15(6): 1100 ～ 1104(in Chinese)
[27] Xiao H(肖红),Zhou Q-X(周启星),CaoYing(曹莹),et al.2003. Effects of different application dosage of herbicide on rice production. Chin J Appl Ecol (应用生态学报), 14(4) :601 ～ 603(in Chinese)
[28] Zhang WM, Watson AK. 1997. Characterization of growth and conidia production of Exserohilum monoceras on different substrates. Biocont Sci Technol, 7:75～86
[29] Zhang WM, Watson AK. 1997. Efficacy of Exserohilum monoceras for the control of Echinochloa species in rice (Oryza sativa).Weed Sci, 45 (1): 144 ～ 150