关键词: Bt毒蛋白, 亚洲玉米螟, 粪便, 转移积累, 个体大小不一致性, 间伐方式, 干扰树间伐, 洛伦茨不对称系数

Abstract: Taking the insect-resistant transgenic corn varieties G03-2396 and G03-2739 and the conventional corn variety Suyu 16 as test materials, a bioassay in laboratory was conducted to test their resistance against  Ostrinia furnacalis. The Bt toxin expression in different tissues of the two transgenic corns, the ingestion of  Bt  toxin by the 3rd and 5th instar of O. furnacalis, and the  Bt  toxin amount in feces of O. furnacalis larvae fed with  Bt  corns were analyzed by using enzyme-linked-immunosorbent-assay (ELISA). It was found that the central leaves of both G03-2396 and G03-2739 had great toxicity to O. furnacalis larvae. After fed with the central leaves for 6 d, the survival rate of neonate larvae was less than 3%, and that of the 3rd instar larvae was less than 70%. Female ears had smaller toxicity than central leaves. The  Bt  toxic protein was detected both in the leaves and in the female ears of the two transgenic corns, but its content was higher in central leaves than in female ears. The Bt  toxic protein expression level was in  order of G03-2739 central leaf (39.6 μg·g^-1 FM) > G03-2396 central leaf (26.1 μg·g^-1 FM) > G03-2396 female ear (17.0 μg·g^-1 FM) > G03-2739 female ear (14.6 μg·g^-1 FM). When fed with central leaf or female ear, the  Bt toxic protein amount in the 3rd instar larvae was significantly higher than that in the 5th instar larvae. For the same age instar larvae, the  Bt  toxic protein content was significantly higher after fed with central leaf than fed with female ear, so did the  Bt toxic protein content in larvae feces, being the highest (10.4 μg·g^-1 FM) for the 5th instar larvae fed with G03-2739 central leaf, and the lowest (2.7 μg·g^-1 FM) for the 3rd instar larvae fed with G03-2739 female ear.

Key words: insect-resistant transgenic corn, Bt toxic protein, Ostrinia furnacalis, feces, transfer and accumulation, individual size inequality, thinning regime, crop tree release, Lorenz asymmetry coefficient.