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应用生态学报 ›› 2010, Vol. 21 ›› Issue (07): 1829-1834.

• 研究报告 • 上一篇    下一篇

硫对铅污染水稻土微生物活性及群落结构的影响

林惠荣1,施积炎1,2**,傅晓萍1,杨建军1,陈英旭2   

  1. 1浙江大学环境保护研究所, 杭州 310029;2农业部面源污染控制重点开放实验室, 杭州 310029
  • 出版日期:2010-07-20 发布日期:2010-07-20

Effects of sulfur on microbial activity and community structure in a lead-polluted paddy soil.

LIN Hui-rong1, SHI Ji-yan1,2, FU Xiao-ping1, YANG Jian-jun1,CHEN Ying-xu2   

  1. 1Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310029, China;2Ministry of Agriculture Key Laboratory of Non-point Source Pollution Control, Hangzhou 310029, China
  • Online:2010-07-20 Published:2010-07-20

摘要: 对含有不同浓度铅的水稻土添加硫代硫酸钠进行土培试验,研究硫作用下铅污染水稻土微生物活性及群落结构的变化.结果表明:添加硫底物后,土壤的氧化还原电位(Eh)升高,呼吸强度增强,硫氧化菌的数量显著增加.硫促进了土壤中硫氧化菌等微生物的生长并引起群落结构的变化,克隆测序表明,加硫处理下土壤微生物的特异性条带与拟杆菌、硫杆菌、β-变形菌和嗜酸菌具有很高的相似性.硫处理下土壤碳酸盐结合态及铁锰结合态铅含量发生了显著变化.

关键词: 稻田土壤, 铅, 硫, 微生物群落, 硫氧化菌, 贵阳市, 城市扩展, 空间模拟, 优化路径

Abstract: A pot experiment was conducted to study the microbial activity and community structure in a lead-polluted paddy soil under effects of amendment with
different concentration sodium thiosulfate. The amendment of the sulfur-containing substrate increased the soil oxidation-reduction potential and respiration rate, promoted the growth of soil sulfur-oxidizing bacteria, and induced some changes in soil microbial community structure. The clone sequencing indicated that the specific bands of soil microbes in sodium thiosulfate treatments had very high similarity to Bateroidetes, Thiobacillus, β-proteobacteria, and Acidobacteria. Significant changes were observed in the contents of soil carbonate- and Fe/Mn oxide- bound Pb after the amendment of sodium thiosulfate.

Key words: paddy soil, lead, sulfur, microbial community, sulfur-oxidizing bacteria, Guiyang City, urban expansion, space simulation, optimal path.