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应用生态学报 ›› 2010, Vol. 21 ›› Issue (08): 2038-2044.

• 研究报告 • 上一篇    下一篇

根结线虫对黄瓜叶片氮磷含量、土壤pH和酶活性的影响

许 华1,2,阮维斌1**,高玉葆1,宋晓艳1,魏宇昆1   

  1. 1南开大学生命科学学院,天津 300071|2北京师范大学珠海分校工程技术学院,广东珠海 519085
  • 出版日期:2010-08-18 发布日期:2010-08-18

Effects of root-knot nematodes on cucumber leaf N and P contents, soil pH, and soil enzyme activities.

XU Hua1,2, RUAN Wei-bin1, GAO Yu-bao1, SONG Xiaoyan1, WEI Yu-kun1   

  1. 1College of Life Sciences, Nankai University, Tianjin 300071, China;2College of Engineering Technology, Beijing Normal University Zhuhai Campus, Zhuhai 519085, Guangdong, China
  • Online:2010-08-18 Published:2010-08-18

摘要: 通过盆栽试验,采用人工接种的方法研究了接种根结线虫对黄瓜植株根际和非根际土壤pH值、土壤酶活性及叶片N、P含量的影响.结果表明:根际土壤pH值在接种量达到每株6000个时才显著降低;随着接种量的增加,叶片N、P含量、根际土壤过氧化物酶、根际和非根际土壤多酚氧化酶活性逐渐降低,根际土壤过氧化氢酶活性却逐渐升高,非根际土壤pH值先升高后降低,非根际土壤过氧化氢酶活性没有明显的变化规律.接种根结线虫后,根际土壤脲酶活性显著降低,根际和非根际土壤磷酸酶活性、非根际土壤过氧化物酶活性仅在较高接种量时才显著降低.根际土壤pH值、酶活性及叶片N、P含量之间多数呈极显著相关,而非根际土壤pH值、酶活性及叶片N、P含量之间只有部分呈极显著相关.

关键词: 根结线虫, 黄瓜, 土壤酶, pH, 氮, 磷, 长期施肥, 农田黑土, 土壤微生物, Biolog-ECO, 土壤养分

Abstract: A pot experiment was conducted to study the effects of inoculation with root-knot nematodes on the cucumber leaf N and P contents, and the rhizospheric and non-rhizospheric soil pH and enzyme activities. The rhizospheric soil pH didn’t have a significant decrease until the inoculation rate reached 6000 eggs per plant. With the increase of inoculation rate, the leaf N and P contents, rhizospheric soil peroxidase activity, and rhizospheric and non-rhizospheric soil polyphenol oxidase activity all decreased gradually, rhizospheric soil catalase activity was in adverse, non-rhizospheric soil pH decreased after an initial increase, and non-rhizospheric soil catalase activity had no regular change. After inoculation, rhizospheric soil urease activity decreased significantly, but rhizospheric and non-rhizospheric soil phosphatase activity and non-rhizospheric soil peroxidase activity only had a significant decrease under high inoculation rate. In most cases, there existed significant correlations between rhizospheric soil pH, enzyme activities, and leaf N and P contents; and in some cases, there existed significant correlations between non-rhizospheric soil pH, enzyme activities, and leaf N and P contents.

Key words: root-knot nematode, cucumber, soil enzyme, pH, N, P, long-term fertilization, arable black soils, soil microorganism, biolog-eco, soil nutrient