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应用生态学报 ›› 2011, Vol. 22 ›› Issue (04): 936-942.

• 研究报告 • 上一篇    下一篇

不同浓度Ni、Cu处理对骆驼蓬光合作用和叶绿素荧光特性的影响

鲁艳1,2**,李新荣3,何明珠3,苏延桂1,曾凡江1,2   

  1. 1中国科学院新疆生态与地理研究所, 乌鲁木齐 830011;2新疆策勒荒漠草地生态系统国家野外科学观测研究站, 新疆策勒 848300;3中国科学院寒区旱区环境与工程研究所沙坡头沙漠研究试验站, 兰州 730000
  • 出版日期:2011-04-18 发布日期:2011-04-18

Influence of different concentration Ni and Cu on the photosynthesis and chlorophyll fluorescence characteristics of Peganum harmala.

LU Yan1,2, LI Xin-rong3, HE Ming-zhu3, SU Yan-gui1, ZENG Fan-jiang1,2   

  1. 1Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China|2Cele National Station of Observation &Research for Desert-Grassland Ecosystem in Xinjiang, Cele 848300, Xinjiang, China|3Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
  • Online:2011-04-18 Published:2011-04-18

摘要: 以骆驼蓬幼苗为材料,采用盆栽试验研究不同浓度(0、50、100、200、400 mg·kg-1)Ni、Cu处理对骆驼蓬叶片光合作用、叶绿素荧光特性及生长状况的影响.结果表明: 随着Ni浓度的增加,骆驼蓬幼苗叶片的光合色素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、PSⅡ最大光化学效率(Fv/Fm)、PSⅡ电子传递量子产率(ΦPSⅡ)、光化学猝灭系数(qP)及各项生长指标均呈显著下降趋势,而细胞间隙CO2浓度(Ci)和非光化学猝灭系数(qN)呈显著增加趋势,其中Pn的下降主要是由非气孔限制所致;骆驼蓬幼苗叶片的光合色素含量、PnGsTrCiFv/FmΦPSⅡqP及各项生长指标均在50 mg·kg-1 Cu处理时达到峰值,叶绿素a和b、PnGsTrCiFv/Fm及各项生长指标值在100 mg·kg-1 Cu处理时仍微高于对照,而后随Cu浓度的增加,光合色素含量、PnGsTrCiFv/FmΦPSⅡqP及各项生长指标均呈下降趋势,qN呈增加趋势,其中Pn的下降主要是由气孔限制所致.

关键词: 光合作用, 叶绿素荧光, 骆驼蓬, 镍, 铜  

Abstract: A pot experiment was conducted to study the influence of different concentration (0, 50, 100, 200, and 400 mg·kg-1) Ni and Cu on the growth, photosynthesis, and chlorophyll fluorescence characteristics of Peganum harmala seedlings. With increasing concentration Ni in the medium, the seedlings growth parameters, photosynthetic pigment content, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximal photochemical efficiency of PSⅡ (Fv/Fm), quantum efficiency of electric transport of PSⅡ(ΦPSⅡ), and coefficient of photochemical quenching (qP) of P. harmala decreased significantly, while the intercellular CO2 concentration (C) and the coefficient of non-photochemical quenching (qN) were in adverse. The decrease of Pn under Ni stress was mainly caused by non-stomatal limitation. At 50 mg·kg-1 Cu, the growth parameters, photosynthetic pigment content, Pn, Gs, Tr, Ci, Fv/Fm, ΦPSⅡ, and qP reached their peak values; at 100 mg·kg-1 Cu, the growth parameters, chlorophyll a and b contents, Pn, Gs, Tr,C, and Fv/Fm were still slightly higher than the control; while with the further increasing Cu concentration in the medium, all the test indices except qN tended to decrease. The decrease of Pn under Cu stress was mainly caused by stomatal limitation. 

Key words: photosynthesis, chlorophyll fluorescence, Peganum harmala, nickel, copper