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应用生态学报 ›› 2011, Vol. 22 ›› Issue (06): 1573-1578.

• 研究报告 • 上一篇    下一篇

香蕉轮作和连作土壤细菌主要类群

欧阳娴,阮小蕾,吴超,白亭亭,李华平**   

  1. 华南农业大学资源环境学院, 广州 510642
  • 出版日期:2011-06-18 发布日期:2011-06-18

Main bacterial groups in banana soil under rotated and continuous cropping.

OUYANG Xian, RUAN Xiao-lei, WU Chao, BAI Ting-ting, LI Hua-ping   

  1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
  • Online:2011-06-18 Published:2011-06-18

摘要: 香蕉枯萎病是香蕉生产上最主要病害,而利用韭菜轮作能有效防控香蕉枯萎病的发生.本文直接对香蕉-韭菜轮作、香蕉连作的土壤样品抽提总DNA,并对总DNA的细菌16S rDNA V3高变区域序列进行PCR扩增,扩增产物经变性梯度凝胶电泳(DGGE)进行分离后,对两类土壤中主要差异条带进行回收测序,经NCBI比对分析来鉴定细菌类群.结果表明:韭菜轮作地的细菌多样性更为丰富,并且以拟杆菌门、变形菌门、放线菌门、绿湾菌门和酸杆菌门为主要类群;而香蕉连作地细菌多样性有所减少,但有明显的优势种群出现,以厚壁菌门、变形菌门、放线菌门和绿湾菌门为主要菌群.

关键词: 香蕉枯萎病, 香蕉-韭菜轮作, 细菌多样性, 变性梯度凝胶电泳

Abstract: Banana wilt is the main disease in banana production, while banana-leek rotation can effectively control the occurrence of the disease. In order to understand the variations of soil bacterial groups under banana-leek rotation and banana continuous cropping, soil samples under these two cropping systems were collected to extract crude DNA, and the bacterial 16S rDNA in V3 region was amplified by PCR. The PCR products were then separated by DGGE, and the main different bands were sequenced and compared with the records of NCBI to identify the germs. Under banana-leek rotation, soil bacterial diversity was richer, and the main bacterial groups were Bacteroidetes, Proteobacteria, Actinobacteria, Chloroflexi, and Acidobacteria; while under banana continuous cropping, the soil bacterial diversity was somewhat decreased, and the main bacterial groups were Firmicutes, Proteobacteria, Actinobacteria, and Chloroflexi.

Key words: banana wilt, banana-leek rotation, bacterial diversity, DGGE