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应用生态学报 ›› 2011, Vol. 22 ›› Issue (10): 2539-2545.

• 研究论文 • 上一篇    下一篇

腐植酸浸种对盐碱胁迫下小麦幼苗抗氧化系统的影响

郭伟1,2 ,王庆祥1**   

  1. 1沈阳农业大学农学院, 沈阳 110161;2黑龙江八一农垦大学农学院, 黑龙江大庆 163319
  • 出版日期:2011-10-18 发布日期:2011-10-18

Effects of seed soaking with humic acid on wheat seedlings antioxidant system under salt-alkali stress.

GUO Wei1,2, WANG Qing-xiang1   

  1. 1College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China;2College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Online:2011-10-18 Published:2011-10-18

摘要: 对龙麦26和克旱16两种不同基因型春小麦品种进行腐植酸浸种处理,研究了腐植酸对盐(NaCl)、碱(Na2CO3)两种胁迫下小麦幼苗抗氧化系统的影响.结果表明:盐碱胁迫下小麦幼苗叶片脯氨酸含量增加,膜透性增强,地上部鲜质量降低;盐胁迫下,过氧化氢酶(CAT)活性升高,过氧化物酶(POD)活性降低,超氧化物歧化酶(SOD)活性先升后降,而碱胁迫下CAT和POD活性均升高,SOD活性降低;碱胁迫处理的小麦幼苗叶片抗氧化酶系统活性高于同浓度的盐胁迫处理;腐植酸浸种提高了小麦叶片谷胱甘肽含量,提高了SOD和CAT活性,有效缓解了盐碱胁迫对幼苗生长的影响.不同基因型小麦品种在抵御盐碱胁迫的能力和机制上存在一定差异,且与盐离子组成、浓度间存在一定互作,并受浸种方式调控.

Abstract: The seeds of two spring wheat genotypes Longmai 26 and Kehan 16 were soaked with humic acid to study the effects of seed soaking with humic acid on the seedlings antioxidant system of the two genotypes under NaCl and Na2CO3 stresses. Under the salt and alkali stresses, the seedlings leaf proline (Pro) content increased, membrane permeability enhanced, and aboveground fresh mass decreased. Under NaCl stress, the leaf catalase (CAT) activity increased, peroxidase (POD) activity decreased, and superoxide dismutase (SOD) decreased after an initial increase; under Na2CO3 stress, the CAT and POD activities increased while the SOD activity was in adverse. The activities of the test antioxidant enzymes were higher under Na2CO3 than under NaCl stress. When the seeds were soaked with humic acid, the seedlings leaf glutathione (GSH) content increased, and the SOD and CAT activities improved, which effectively relieved the damage of salt-alkali stress on the seedlings growth. Some differences were observed in the resistance level and related mechanisms against the salt-alkali stress between the two wheat genotypes, possibly due to their different interactions with the salt ion components and concentrations and the ways of seed soaking.