解磷菌株B25的筛选、鉴定及其解磷能力

应用生态学报 ›› 2012, Vol. 23 ›› Issue (01): 235-239.

解磷菌株B25的筛选、鉴定及其解磷能力

贺梦醒,高毅,胡正雪,徐瑶,龙瑞,孙庆业**

安徽大学资源与环境工程学院，合肥 230601

出版日期:2012-01-18 发布日期:2012-01-18

Screening, identification, and phosphate-solubilizing capability of phosphate-solubilizing bacterial strain B25.

HE Meng-xing, GAO Yi,HU Zheng-xue, XU Yao, LONG Rui, SUN Qing-ye

School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China

Online:2012-01-18 Published:2012-01-18

摘要/Abstract

摘要： 从安徽省铜陵市铜官山尾矿库木贼根际分离筛选出多株解磷细菌，经过多次筛选纯化获得一株解磷能力较好的菌株B25.采用透射电镜观察和DNA分子技术，确定此菌株属于芽孢杆菌属.研究了解磷菌株B25在培养168 h内的解磷能力、溶液pH值以及菌株生长量的变化情况，并比较了B25在不同条件下的解磷能力.结果表明：解磷菌株B25的解磷能力与溶液pH值之间存在微弱的相关性，在碳源为葡萄糖、初始pH值为7.0、培养温度为30 ℃时解磷效果较好.

关键词: 解磷菌, 解磷能力, 16S rRNA

Abstract: Various phosphate-solubilizing bacterial strains were isolated from the Hippochaete ramosissimum rhizosphere in Tongguanshan copper tailings in Tongling of Anhui Province, East China. After many times of screening and purification, a strain B25 with stronger phosphate-solubilizing capability was obtained, which belonged to Bacillus genus, as identified by transmission electron microscope and DNA molecular approaches. A culture experiment was conducted to study the phosphate-solubilizing capability of the B25 within 168 h and the variations of the medium pH and B25 growth as well as the phosphate-solubilizing capability of B25 under different culture conditions. A weak correlation was observed between the phosphate-solubilizing capability of B25 and the medium pH. The B25 displayed a better phosphate-solubilizing capability when the carbon source was glucose, medium initial pH was 7.0, and culture temperature was 30 ℃.

Key words: phosphate-solubilizing bacteria, phosphate-solubilizing capability, 16S rRNA

贺梦醒,高毅,胡正雪,徐瑶,龙瑞,孙庆业. 解磷菌株B25的筛选、鉴定及其解磷能力[J]. 应用生态学报, 2012, 23(01): 235-239.

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