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应用生态学报 ›› 2012, Vol. 23 ›› Issue (03): 807-811.

• 研究论文 • 上一篇    下一篇

假单胞菌菌株CTN-3对百菌清污染土壤的生物修复

王光利1,2,陈宏宏1,毕〓萌1,李顺鹏1**   

  1. 1南京农业大学生命科学院农业部农业环境微生物工程重点开放实验室, 南京 210095; 2淮北师范大学生命科学学院资源植物学安徽省重点实验室, 安徽淮北 235000
  • 出版日期:2012-03-18 发布日期:2012-03-18

Bioremediation of chlorothalonil-contaminated soil by utilizing Pseudomonas sp. strain CTN-3.

WANG Guang-li1,2, CHEN Hong-hong1, BI Meng1, LI Shun-peng1   

  1. 1Ministry of Agriculture Key Laboratory of Microbiological Engineering Agricultural Environment, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2Anhui Province Key Laboratory of Plant Resources and Biology, College of Life Sciences, Huaibei Normal University, Huaibei 235000, Anhui, China
  • Online:2012-03-18 Published:2012-03-18

摘要: 百菌清被美国环境保护署列为优先控制污染物,利用微生物的降解作用修复被污染的土壤、清除环境中的污染物等具有重要的现实意义.假单胞菌(Pseudomonas sp.)菌株CTN-3是一株从污染土壤中分离得到的百菌清降解菌,考察了其在实验室条件下对百菌清污染土壤的生物修复能力及其影响因素.结果表明: 降解菌株在灭菌土壤中的降解效果略好于未灭菌土壤;在外源添加降解菌106 CFU·g-1、温度15~30 ℃和pH 5.8~8.3条件下,该菌株能有效降解土壤中10~200 mg·kg-1的百菌清.菌株CTN-3在百菌清污染土壤的生物修复中具有良好的应用前景.

关键词: 百菌清, 假单胞菌, 生物修复, 影响因素

Abstract: Chlorothalonil is the priority organic pollutant listed by the U.S. Environmental Protection Agency. To utilize the function of microbial degradation in the bioremediation of chlorothalonil-contaminated soil is of practical significance. In this study, a chlorothalonil-degrading Pseudomonas sp. strain CTN-3 isolated from pesticide-contaminated soil was used to examine the chlorothalonil- degrading capacity of the strain and related affecting factors in a microcosm. In sterilized soil, the effect of CTN-3 on chlorothalonil degradation was better than that in unsterilized soil. Various factors, including soil pH, temperature, initial chlorothalonil concentration, and inoculum size, affected the degradation of chlorothalonil by the strain. With the inoculum size of 106 CFU·g-1 soil, the CTN-3 at 15-30 ℃ and pH 5.8-8.3 could effectively degrade 10-200 mg·kg-1 of chlorothalonil, suggesting that the strain CTN-3 had great potential in the bioremediation of chlorothalonil-contaminated soil.

Key words: chlorothalonil,  Pseudomonas sp., bioremediation, affecting factor