关键词: 镉, 霞多丽, 亚细胞分布, 化学形态

Abstract: A pot culture experiment was conducted to study the Cd absorption, Cd subcellular distribution, and Cd chemical forms in oneyear old selfrooted Chardonnay and SO4 rootstock-grafted Chardonnay grapevine after root-irrigating with different concentration CdCl₂ and CaCl₂. In the grapevine, the absorbed Cd was mostly distributed in underground organs (root and rhizome). In treatment 4 mmol·L^-1 of CdCl₂, 77.1% and 1.4% of the absorbed Cd in selfrooted Chardonnay were accumulated in underground organs and leaves, respectively, while 93.9% and 0.1% of the absorbed Cd in grafted Chardonnay were accumulated in the organs below graft position and in leaves, respectively. 5 mmol·L^-1 of CaCl₂ decreased the plant Cd absorption and accumulation, while 10 mmol·L^-1 of CaCl₂ increased the plant Cd absorption and accumulation significantly. The Cd subcellular distribution in roots and leaves was in the order of cell wall > soluble fraction > organelle, and more than 50% of the Cd was accumulated in cell wall. In the roots, NaCl-extractable Cd had a major proportion, followed by HAc-extractable Cd, and waterextractable Cd. The contents of all the Cd chemical forms varied with the increasing concentration of Cd in the treatments.

Key words: cadmium, Chardonnay, subcellular distribution, chemical form.