云南滇池富磷区趋化性细菌的系统多样性

应用生态学报 ›› 2012, Vol. 23 ›› Issue (07): 1985-1991.

云南滇池富磷区趋化性细菌的系统多样性

郝玉娥¹,杨培香²,陈明会²,舒雪萍²,陈强²,李程¹,杨发祥³,莫明和^2**

(¹College of Public Health, University of South China, Hengyang 421001, Hunan, China; ²Key Laboratory for Conservation and Utilization of BioResources, Yunnan University, Kunming 650091, China; ³Yunnan Microbial Fermentation Engineering Research Center Co. Ltd., Kunming 650217, China)

出版日期:2012-07-18 发布日期:2012-07-18

Phylogenic diversity of soil chemotatic bacteria in a phosphorous-rich area around Dianchi Lake of Yunnan Province, Southwest China.

(¹南华大学公共卫生学院，湖南衡阳 421001; ²云南大学生物资源保护与利用重点实验室，昆明 650091; ³云南省微生物发酵工程研究中心有限公司，昆明650217)

Online:2012-07-18 Published:2012-07-18

摘要/Abstract

摘要： 从云南滇池富磷区100份土样中筛选解磷细菌(PSB)，通过组氨酸激酶编码基因(cheA)筛选趋化性PSB，并通过软琼脂平板法验证其趋化性；利用钼蓝比色法测定PSB对磷酸三钙的溶解能力；基于16S rRNA基因序列分析趋化性PSB的系统亲缘关系.结果表明：分离到的145株PSB的溶磷圈直径在0.5～2 cm，其中37株为趋化性PSB.该37株PSB对供试的4种趋化底物均具有趋化性，而且对磷酸三钙均具有解磷活性.基于16S rRNA基因序列的系统发育分析显示，这37株趋化性PSB分属于10属，共17种细菌，其中假单胞菌属种类最多(5种9株)，肠杆菌属次之(3种8株)，芽孢杆菌属尽管只分离到1个种(Bacillus aryabhattai)，但共分离到9个菌株.

关键词: 滇池富磷区, 解磷细菌, 趋化性, 系统多样性

Abstract: Phosphate-solubilizing bacteria (PSB) were isolated from 100 soil samples collected from a phosphorous-rich area around the Dianchi Lake of Yunnan Province, Southwest China. The chemotatic PSB strains were screened by cheA gene detection, and their chemotaxis was verified by the method of soft agar plate. The tricalcium phosphate (TCP)solubilizing activities of PSB were determined with molybdenum blue spectrophotometry. Based on 16S rRNA sequences, the phylogenic relationships of the PSB were analyzed. A total of 145 PSB strains with a diameter of phosphatesolubilizing halo zone ranged from 0.5 cm to 2 cm were isolated, among which, 37 strains were chemotactic. The 37 chemotactic strains showed chemotaxis towards four test attractants, and exhibited TCPsolubilizing activity. Phylogenic analysis revealed that the 37 chemotatic strains were belonged to 17 species of 10 genera, in which, Pseudomonas was dominant (9 strains of 5 species), followed by Enterobacter (8 strains of 3 species). Only one species (Bacillus aryabhattai) was isolated from Bacillus, but 9 strains were identified.

Key words: phosphorous-rich area around Dianchi Lake, phosphate-solubilizing bacteria, chemotaxis, phylogenetic diversity.

郝玉娥1,杨培香2,陈明会2,舒雪萍2,陈强2,李程1,杨发祥3,莫明和2**. 云南滇池富磷区趋化性细菌的系统多样性[J]. 应用生态学报, 2012, 23(07): 1985-1991.

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