西花蓟马对吡虫啉、辛硫磷和甲维盐的抗性风险和抗性稳定性

应用生态学报

西花蓟马对吡虫啉、辛硫磷和甲维盐的抗性风险和抗性稳定性

王圣印¹,于毅²,刘永杰^1**,马井玉³

(1山东农业大学植物保护学院, 山东泰安 271018； ²山东省农业科学院植物保护研究所, 济南 250100； ³济宁市农业科学研究院, 山东济宁 272031)

出版日期:2012-12-18 发布日期:2012-12-18

Resistance risk and resistance stability of Frankliniella occidentalis to imidacloprid, emamectin benzoate, and phoxim.

WANG Sheng-yin¹, YU Yi², LIU Yong-jie¹, MA Jing-yu³

(¹College of Plant Protection, Shandong Agricultural University, Tai’an 271018, Shandong, China; ²Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China; ³Ji’ning Academy of Agricultural Sciences, Ji’ning 272031, Shandong, China)

Online:2012-12-18 Published:2012-12-18

摘要/Abstract

摘要： 为有效控制西花蓟马的危害,采用吡虫啉、辛硫磷和甲维盐药液浸泡芸豆法,分别对西花蓟马敏感种群成虫进行抗性选育,获得抗性种群,应用抗性现实遗传力分析抗性遗传和抗性风险.结果表明: 经过32代、32代和24代抗性选育,西花蓟马对吡虫啉、辛硫磷和甲维盐的抗性分别达到13.8、29.4和39.0倍.西花蓟马对吡虫啉、辛硫磷和甲维盐的现实抗性遗传力分别为0.112、0.166和0.259,西花蓟马对甲维盐的抗性上升速度最快,辛硫磷次之,吡虫啉最低.经过药剂筛选后,西花蓟马若虫与成虫对杀虫剂的敏感性差异较敏感种群显著缩小.停止用药剂筛选后继续饲养12代,西花蓟马对吡虫啉、辛硫磷和甲维盐的抗性水平都有一定程度下降,但都没有恢复到敏感性水平.西花蓟马对吡虫啉、辛硫磷和甲维盐具有较高水平抗性的风险,其中对吡虫啉的抗性发展速度相对较慢,且下降速度较快,因此选用吡虫啉防治西花蓟马更合适.

Abstract: In order to effectively control the damage of Frankliniella occidentalis (Pergande), Phaseolus vuglaris was dipped with imidacloprid, phoxim, and emamectin benzoate, respectively to select the resistance populations of F. occidentalis from its susceptible population, and the resistance inheritance and resistance risk were analyzed with the resistance reality heredity. After 32, 32, and 24 generations’ selection, the F. occidentalis populations obtained 13.8fold, 29.4fold and 39.0fold resistance to imidacloprid, phoxim, and emamectin benzoate, respectively. The resistance reality heritability to imidacloprid, phoxim, and emamectin benzoate was 0.112, 0.166, and 0.259, respectively. The resistance development rate to emamectin benzoate was the fastest, followed by to phoxim, and to imidacloprid. The higher the resistance levels of the selected populations, the lower the differences between the larva and adult susceptibility to imidacloprid, phoxim, and emamectin benzoate. Stopping selection for 12 continuous generations, the resistance level of the selected resistance populations to imidacloprid, phoxim, and emamectin benzoate had definite decline, but it was difficult to regain the original susceptibility. F. occidentalis had a greater potential to gain high level resistance to imidacloprid, phoxim, and emamectin benzoate. Compared with the resistance of F. occidentalis to phoxim and emamectin benzoate, the resistance to imidacloprid increased slower and decreased faster, and thus, imidacloprid was more appropriate to control F. occidentalis in practice.

王圣印1,于毅2,刘永杰1**,马井玉3. 西花蓟马对吡虫啉、辛硫磷和甲维盐的抗性风险和抗性稳定性[J]. 应用生态学报.

WANG Sheng-yin1, YU Yi2, LIU Yong-jie1, MA Jing-yu3. Resistance risk and resistance stability of Frankliniella occidentalis to imidacloprid, emamectin benzoate, and phoxim.[J]. Chinese Journal of Applied Ecology.