天目山不同海拔檫木群体遗传多样性和遗传结构

doi:10.13287/j.1001-9332.201606.012

摘要/Abstract

摘要： 采用13对SSR引物,运用Bioptic Qsep100全自动核酸分析系统,分析了天目山5个海拔檫木群体的遗传多样性和遗传结构及其在不同海拔下的变化模式.结果表明: 天目山檫木群体具有较高的遗传多样性水平,其中期望杂合度和观察杂合度分别为0.84和0.61.根据Shannon指数,天目山檫木中海拔(500～800 m)群体的遗传多样性水平大于低海拔(200 m)和高海拔(1100～1400 m)群体的遗传多样性水平.由基因分化系数和AMOVA分析可知,檫木种群的遗传变异主要存在于群体内.STRUCTURE分析和UPGMA聚类分析表明,中、低海拔被划为一个群体,而高海拔被划为另一个群体.低海拔和中海拔檫木群体遗传距离的差异表明,人为干扰对物种多样性具有负面效应,而自然保护区对物种多样性的保护起到了积极作用.

Abstract: To analyze the genetic diversities and structures of Sassafras tsumu and to explore their changing patterns at five different altitudes, Bioptic Qsep 100 DNA fragment analyzer combined with 13 SSR primers was applied. The results showed that S. tsumu in Tianmushan Mountain had a relatively high genetic diversity, with the expected heterozygosity (H_e) and observed heterozygosity (H_o) being 0.84 and 0.61, respectively. According to Shannon index (I), the diversity of S. tsumu at middle altitude (500-800 m) was higher than that at low (200 m) and high altitudes (1100-1400 m). The coefficient of genetic differentiation and AMOVA results indicated that the genetic variation of S. tsumu was mainly distributed within populations. UPGMA cluster analysis and STRUCTURE analysis showed that S. tsumu populations at the middle and lower altitudes were similar, but different from that at higher altitude. The differences in genetic distance of S. tsumu populations at middle and low altitudes revealed that human disturbance posed a negative effect on the diversity, whereas, natural reserve played a significant positive role in protecting species diversity.

蒋艾平,姜景民,刘军,. 天目山不同海拔檫木群体遗传多样性和遗传结构[J]. 应用生态学报, 2016, 27(6): 1829-1836.

JIANG Ai-ping, JIANG Jing-min, LIU Jun. Genetic diversity and genetic structure in Sassafras tsumu populations along altitudinal gra-dients in Tianmushan Mountain, China.[J]. Chinese Journal of Applied Ecology, 2016, 27(6): 1829-1836.

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