污水与镉复合胁迫对玉米幼苗代谢物的影响

doi:10.13287/j.1001-9332.201711.038

应用生态学报 ›› 2017, Vol. 28 ›› Issue (11): 3767-3774.doi: 10.13287/j.1001-9332.201711.038

污水与镉复合胁迫对玉米幼苗代谢物的影响

李萌, 陈佳月, 解静芳^*, 吕佳莉, 高少龙, 刘瑞卿

山西大学环境与资源学院, 太原 030006

出版日期:2017-11-18 发布日期:2017-11-18
通讯作者: *mail:xiejf@sxu.edu.cn
作者简介:李萌, 女, 1992年生, 硕士研究生.主要从事环境毒理学与化学研究.E-mail:85380631@qq.com
基金资助:
本文由国家自然科学基金项目(30740037)和国家公益性行业(农业)科研专项(201103024)资助

Effects of sewage and Cd combined stress on metabolites of maize seedling

LI Meng, CHEN Jia-yue, XIE Jing-fang^*, LYU Jia-li, GAO Shao-long, LIU Rui-qing

College of Environment and Resource, Shanxi University, Taiyuan 030006, China

Online:2017-11-18 Published:2017-11-18
Contact: *mail:xiejf@sxu.edu.cn
Supported by:
This work was supported by the Natio-nal Natural Science Foundation of China (30740037) and the Special Fund for Agro-scientific Research in the Public Interest (201103024)

摘要/Abstract

摘要： 运用GC-MS技术结合AMDIS软件对代谢物种类、含量及其代谢途径进行分析,研究污水与镉复合胁迫对玉米幼苗代谢物的影响.共鉴定出50种代谢物.在镉单一胁迫下,玉米幼苗苹果酸含量降低,柠檬酸含量升高,植株体内三羧酸循环稳定;在污水与镉复合胁迫下,柠檬酸和苹果酸含量均显著增加,复合胁迫阻碍了三羧酸循环的正常进行.镉单一胁迫和污水与镉复合胁迫均导致葡萄糖含量显著下降,与抗逆相关的物质腐胺、脯氨酸、肌醇和γ-氨基丁酸含量均显著升高,表明光合作用受到抑制,但以复合胁迫的变化更为明显.表明污水与镉复合胁迫对玉米幼苗代谢物影响的作用机制主要是通过阻碍三羧酸循环和抑制光合作用进行.

Abstract: To investigate the influences of sewage and cadmium combined stress on maize seedling metabolites, we took GC-MS metabonomics technology to analyze the metabolite species, content and metabolic pathways. A total of 50 kinds of metabolites were identified. Under single stress of Cd, the malic acid content in maize seedling decreased, the citric acid content increased significantly, and the tricarboxylic acid cycle was relatively stable. Under the combined stress, both malic acid and citric acid contents increased significantly, and the tricarboxylic acid cycle was inhibited. Both single Cd stress and combined stress of sewage and Cd, especially the latter, led to the decrease of glucose and the increase of those substances related to resistance, such as proline, putrescine, muscle and γ-aminobutyric acid, which indicated the photosynthesis was inhibited. It was concluded that sewage and cadmium combined stress influence on maize seedling metabolites mainly by inhibiting the tricarboxylic acid cycle and photosynthesis.

李萌, 陈佳月, 解静芳, 吕佳莉, 高少龙, 刘瑞卿. 污水与镉复合胁迫对玉米幼苗代谢物的影响[J]. 应用生态学报, 2017, 28(11): 3767-3774.

LI Meng, CHEN Jia-yue, XIE Jing-fang, LYU Jia-li, GAO Shao-long, LIU Rui-qing. Effects of sewage and Cd combined stress on metabolites of maize seedling[J]. Chinese Journal of Applied Ecology, 2017, 28(11): 3767-3774.

参考文献

[1] Liu M (刘敏), Zhang C-X (张彩香), Liao X-P (廖小平), et al. Residue characters of OCPs in soil and shallow ground-water of Xiaodian sewage irrigation area, Taiyuan City. Journal of Agro-Environment Science (农业环境科学学报), 2013, 32(9): 1821-1826 (in Chinese)
[2] Hu M-J (胡茂俊), Liu X-H (刘新红), Gao Y (高岩), et al. Effect of microorganism-plant joint remediation on N₂O, N₂ and O₂ release flux from polluted waters in urban river. Journal of Agricultural Resources and Environment (农业资源与环境学报), 2016, 33(1): 35-42 (in Chinese)
[3] Wang H (王涵), Gao S-F (高树芳), Chen Y-H (陈炎辉), et al. Changes of soil enzyme activities in heavy metals polluted region. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(12): 3034-3042 (in Chinese)
[4] Song Y-F (宋玉芳), Zhou Q-X (周启星), Song X-Y (宋雪英), et al. Accumulation of pollutants in sediments and their eco-toxicity in the wastewater irrigation channel of western Shenyang. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(10): 1926-1930 (in Chinese)
[5] Nayek S, Gupta S, Saha RN. Metal accumulation and its effects in relation to biochemical response of vegetables irrigated with metal contaminated water and wastewater. Journal of Hazardous Materials, 2010, 178: 588-595
[6] Wang L (王林), Qin X (秦旭), Xu Y-M (徐应明), et al. Phytoexclusion and in-situ immobilization of cadmium in vegetable field in sewage irrigation region. Journal of Agro-Environment Science (农业环境科学学报), 2014, 33(11): 2111-2117 (in Chinese)
[7] Kiziloglu FM, Turan M, Sahin U, et al. Effects of untreated and treated wastewater irrigation on some chemical properties of cauliflower (Brassica olerecea L. var. botrytis) and red cabbage (Brassica olerecea L. var. rubra) grown on calcareous soil in Turkey. Agricultural Water Management, 2008, 95: 716-724
[8] Abbas ST, Sarfraz M, Mehdi SM, et al. Trace elements accumulation in soil and rice plants irrigated with the contaminated water. Soil and Tillage Research, 2007, 94: 503-509
[9] Liu Y (刘媛), Ma W-C (马文超), Zhang W (张雯), et al. Effect of cadmium stress on root vigor and accumulation of elements Ca, Mg, Mn, Zn, Fe in Salix variegate. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(4): 1109-1115 (in Chinese)
[10] Zhang R-R (张然然), Zhang P (张鹏), Du S-T (都韶婷). Oxidative stress-related signals and their regulation under Cd stress: A review. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(3): 981-992 (in Chinese)
[11] Li H (李贺), Lian H-F (连海峰), Liu S-Q (刘世琦), et al. Effect of cadmium stress on physiological characteristics of garlic seedlings and the alleviation effects of exogenous calcium. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(4): 1193-1198 (in Chinese)
[12] Moussa HR, Gamal SM. Effect of salicylic acid pretreatment on cadmium toxicity in wheat. Biologia Plantarum, 2012, 54: 315-320
[13] Xu J, Yin HX, Li X. Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L. Plant Cell Reports, 2009, 28: 325-333
[14] Aramphongphan A, Laovitthayanggoon S, Himakoun L. Snakeheadfishcell line,SSN-1 (Ophicephalus striatus) as a model for cadmium genotoxicity testing. Toxicology in Vitro, 2009, 23: 963-968
[15] Alvarez SM, Gómez NN, Scardapane L, et al. Effects of chronic exposure to cadmium on prostate lipids and morphology. Biometals, 2007, 20: 727-741
[16] Das P, Samantaray S, Rout G. Studies on cadmium to-xicity in plants: A review. Environmental Pollution, 1997, 98: 29-36
[17] LYU J-L (吕佳莉), Liu X-J (刘小娟), Wang Z-T (王振涛), et al. Study on available contents of Cd and Pb in sewage-irrigated soils in Taiyuan. Journal of Shanxi Agricultural Sciences(山西农业科学), 2015, 12(1): 178-182 (in Chinese)
[18] Wu Y-N (毋燕妮), Han Q (韩琦), Zhang M (张萌), et al. Effects of sewage irrigation and cadmium stresses on the cadmium accumulation and growth process of spinach. Acta Agriculturae Boreali-Sinica (华北农学报), 2014, 29(1): 178-182 (in Chinese)
[19] Cui H-L (崔宏莉). Effects of Sewage and Cd Combined Pollution on Soil and Plant System. Master Thesis. Taiyuan: Shanxi University, 2010 (in Chinese)
[20] Marsolvall A, Balcells M, Eras J, et al. Injection-port derivatization coupled to GC-MS/MS for the analysis of glycosylated and non-glycosylated polyphenols in fruit samples. Food Chemistry, 2016, 204: 210-217
[21] Qi X-Q (漆小泉), Wang Y-L (王玉兰), Chen X-Y (陈晓亚). Plant Metabolomics: Methods and Applications. Beijing: Chemical Industry Press, 2011: 27-45 (in Chinese)
[22] Park SE, Yoo SA, Seo SH, et al. GC-MS based metabolomics approach of Kimchi for the understanding of Lactobacillus plantarum fermentation characteristics. LWT-Food Science and Technology, 2016, 68: 313- 321
[23] Jemmali Z, Chartier A, Dufresne C, et al. Optimization of the derivatization protocol of pentacyclic triterpenes prior to their gas chromatography-mass spectrometry analysis in plant extracts. Talanta, 2016, 147: 35-43
[24] Xue H-B (薛洪宝), Chang H-L (常华兰), Tao Z-L (陶兆林), et al. The interaction between Cd and thiol compounds during the maize seed germination. Journal of Agro-Environment Science(农业环境科学学报), 2011, 30(5): 824-829 (in Chinese)
[25] Potesil D, Petrlova J, Adam V, et al. Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and pyhtochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection. Journal of Chromatography A, 2005, 1084: 134-144
[26] Zhao L-J (赵丽娟), Xie J-F (解静芳), Zhang H (张洪), et al. GC-MS combined with AMDIS analysis of maize seedlings under cadmium stress the influence of the metabolites. Environmental Chemistry(环境化学), 2015, 34(6): 1227-1230 (in Chinese)
[27] Zhao LJ, Xie JF, Zhang H, et al. Changes in metabolites in maize seedlings under chlorsulfuron and cad-mium stress. Agricultural Science, 2015, 154: 1-24
[28] Xue W (薛薇). The Application of Statistical Analysis and SPSS. 2nd Ed. Beijing: China Renmin University Press, 2007: 179-193 (in Chinese)
[29] Li M(李萌), Xie J-F(解静芳), Lyu J-L(吕佳莉), et al. Effects of sewage and Cd compound pollution on maize seedling antioxidant enzyme activity. Journal of Shanxi Agricultural Sciences (山西农业科学), 2017, 45(2): 172-177 (in Chinese)
[30] You R (尤蓉). Metabonomics Investigation on the Mechanisms of Action of Cantonese Herbal Tea. Master Thesis. Guangzhou: South China University of Technology, 2012 (in Chinese)
[31] Wang Z-Q (王志强), Wu Z-H (吴泽华), Li G (李刚), et al. The determination method of threonine content in fermentation liquor. Public Communication of Science & Technology (科技传播), 2010(16): 139-143 (in Chinese)
[32] Hanson AD, Roje S. One-carbon metabolism in higher plants. Annual Review of Plant Physiology & Plant Molecular Biology, 2001, 52: 119-137
[33] Hanson AD, Gregory JF. Folate biosynthesis, turnover, and transport in plants. Annual Review of Plant Biology, 2011, 62: 105-125
[34] Waditee R, Bhuiyan NH, Hirata E, et al. Metabolic engineering for betaine accumulation in microbes and plants. Journal of Biological Chemistry, 2007, 282: 34185-34193
[35] Bannatyne RM, Stringel G, Simpson JS, et al. Arabidopsis phosphoglycerate dehydrogenase1 of the phosphoserine pathway is essential for development and required for ammonium assimilation and tryptophan biosynthesis. Plant Cell, 2013, 25: 5011-5029
[36] Liu QD, Zhu YR, Li HT, et al. Changes of endopeptidase isoenzymes in Spirodela polyrrhiza Thallus during senescence and effect of exogenous L-Serine on endopeptidase isoenzymes. Plant Physiology Communications, 2010, 46: 241-244

污水与镉复合胁迫对玉米幼苗代谢物的影响

Effects of sewage and Cd combined stress on metabolites of maize seedling

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价