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应用生态学报 ›› 2017, Vol. 28 ›› Issue (9): 2984-2992.doi: 10.13287/j.1001-9332.201709.034

• 目次 • 上一篇    下一篇

内生细菌252和254对盐胁迫下小麦幼苗过氧化物酶和过氧化氢酶活性的影响

赵龙飞1*, 徐亚军1, 赖心河2, 常佳丽3, 欧启凡1, 孟嘉琦1, 彭顶华1   

  1. 1商丘师范学院生物与食品学院 河南省高校植物与微生物互作重点实验室, 河南商丘 476000
    2温州医科大学附属第一医院转化医学研究所, 浙江温州 325000
    3清华大学环境学院水环境保护研究所, 北京 100084
  • 收稿日期:2016-10-31 出版日期:2017-09-18 发布日期:2017-09-18
  • 通讯作者: * E-mail: hnzhaolongfei@163.com
  • 作者简介:赵龙飞,男,1978年生,博士,副教授. 主要从事植物内生菌资源与利用研究. E-mail: hnzhaolongfei@163.com
  • 基金资助:

    本文由国家自然科学基金项目(U1204301)、河南省高等学校重点科研项目(17A180011)、国家级大学生创新性实验计划项目(201610483021)和商丘师范学院大学生创新性实验计划重点项目(2016DXS04)资助

Effects of endophytic bacteria 252 and 254 on peroxidase (POD)and catalase (CAT)activities of wheat seedlings under salt stress.

ZHAO Long-fei1*, XU Ya-jun1, LAI Xin-he2, CHANG Jia-li3, OU Qi-fan1, MENG Jia-qi1, PENG Ding-hua1   

  1. 1Key Laboratory of Plant-Microbe Interactions of Henan Universities, College of Biology and Food Sciences, Shangqiu Normal University, Shangqiu 476000, Henan, China;
    2Institute of Translational Medicine, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China;
    3Division of Water Environment Protection, College of Environment, Tsinghua University, Beijing 100084, China.
  • Received:2016-10-31 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: hnzhaolongfei@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (U1204301), University Key Scientific Research Project of Henan Province (17A180011), National Innovation Experiment Program for University Students of China (20161048302) and Innovation Experiment Program for University Students of Shangqiu Normal University(2016DXS04)

摘要: 以‘周麦18’为试验材料,采用盆栽法设置盐胁迫、盐胁迫下接菌和对照3组处理,研究不同盐浓度胁迫下接种内生菌对小麦幼苗生长的影响.结果表明: 内生菌252、254分别与死亡谷芽孢杆菌和根癌土壤杆菌具有最高相似率.盐胁迫下接种内生细菌252,在14 d时小麦幼苗随盐浓度升高过氧化物酶(POD)活性先升高后下降,其中盐浓度为100 mmol·L-1时POD活性最高(13370 U·g-1·min-1);21 d时小麦幼苗POD和过氧化氢酶(CAT)活性较对照明显升高;28 d时盐浓度为150、250 mmol·L-1的POD和CAT活性最高.盐胁迫下接种内生细菌254,在14 d时盐浓度为250 mmol·L-1的小麦幼苗CAT活性最高;21 d时盐浓度在100~250 mmol·L-1的POD和CAT活性较高,其中150 mmol·L-1时CAT活性最高;28 d时盐浓度为200 mmol·L-1的CAT活性最高.在盐胁迫下,接种内生菌对小麦幼苗不同培养时期发挥最佳作用的盐浓度不同.内生菌252和254可提高盐胁迫下小麦植株POD、CAT活性,对小麦幼苗生长有一定修复作用.

Abstract: Taking wheat variety Zhou18 as test material, salt stress, salt stress plus inoculation of endophytic bacteria, and no treatment (control) were set, and the effects of inoculation of endophytic bacteria on the growth of wheat seedlings under salt stress were investigated through pot experiment. The results showed that endophytic bacterial strains 252 and 254 shared the highest similarity with Bacillus vallismortis and Agrobacterium tumefaciens, respectively. On the 14th day, treatment of endophytic bacterium 252 plus salt stress increased POD activity in wheat seedlings with increasing the salt concentration, and showed the highest POD activity (13370 U·g·min-1) at salt concentration of 100 mmol·L-1. On the 21th day, POD and CAT activities in the treated wheat seedlings were significantly higher than those of controlled group, and reached the maximum on the 28th day under salt concentrations of 150 mmol·L-1 and 250 mmol·L-1, respectively. By contrast, wheat seedlings inoculated with strain 254 showed the highest CAT activity under salt concentration 250 mmol·L-1 on the 14th day. Seedlings treated with strain 254 had significantly higher POD and CAT activities at salt concentration 100-250 mmol·L-1 on the 21th day, and CAT activity was the highest at salt concentration of 150 mmol·L-1. On the other hand, CAT activity peaked at salt concentration of 200 mmol·L-1 on the 28th day. Therefore, the repairing effects of inoculated endophytic bacteria on wheat seedlings varied along with its culture stage and salt concentration. Endophytic bacterial strains 252 and 254 could enhance POD and CAT activities in wheat seedlings under salt stress, and exert significant repairing effects on the growth of wheat seedlings.