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应用生态学报 ›› 2018, Vol. 29 ›› Issue (11): 3819-3828.doi: 10.13287/j.1001-9332.201811.040

• 研究报告 • 上一篇    下一篇

内生型解淀粉芽孢杆菌YTB1407对甘薯根系的促生作用及其调控机理

王翠娟1, 王英姿1*, 储昭辉2, 王培松1, 栾炳辉1   

  1. 1山东省烟台市农业科学研究院植物保护研究所, 山东烟台 265500;
    2山东农业大学农学院作物生物学国家重点实验室, 山东泰安 271018
  • 收稿日期:2018-03-26 出版日期:2018-11-20 发布日期:2018-11-20
  • 通讯作者: *E-mail: ytnkyzbs@126.com
  • 作者简介:王翠娟,女,1986年生,博士.主要从事作物生理生态研究. E-mail: cuijuanwangwang@126.com
  • 基金资助:

    本文由烟台市科技计划项目(2017NC022)和国家甘薯产业技术体系烟台综合试验站(CARS-11-C-09-2016)资助

Root promoting effect and its regulation mechanisms of endophytic Bacillus amyloliquefaciens YTB1407 in sweet potato

WANG Cui-juan1, WANG Ying-zi1*, CHU Zhao-hui2, WANG Pei-song1, LUAN Bing-hui1   

  1. 1Institute of Plant Protection, Yantai Academy of Agricultural Sciences, Yantai 265500, Shandong, China;
    2State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2018-03-26 Online:2018-11-20 Published:2018-11-20
  • Contact: *E-mail: ytnkyzbs@126.com
  • Supported by:

    This work was supported by the Science and Technology Planning Project of Yan’tai (2017NC022), and Yan’tai Experimental Station of China Sweet Potato Industrial Technology System (CARS-11-C-09-2016).

摘要: 本实验室从西洋参根中分离筛选获得兼具生防和促生效果的内生型解淀粉芽孢杆菌YTB1407.为调查其应用潜力,以甘薯为材料,以无菌水灌根处理(CK)为对照,采用不同浓度YTB1407菌悬液灌根盆栽甘薯植株,通过对甘薯生长前期3个主要生育时间点YTB1407在植株内的内生定殖检测和根系表型效应比较,筛选菌悬液灌根的最适处理浓度.对根系内源生长素吲哚乙酸(IAA)、细胞分裂素玉米素核苷(ZR)、反式玉米素核糖核苷(t-ZR)的含量和吲哚乙酸氧化酶类中的吲哚乙酸氧化酶(IAAO)、过氧化物酶(POD)和多酚氧化酶(PPO)的活性进行分析.结果表明: YTB1407促进了甘薯生长前期根系统的定殖、不定根幼根的伸长生长及侧根的发生,提高了根系活力;在甘薯生长后期形成了更大的吸收根系生物量和更低的地上部和总根系生物量比.其中OD600值为0.50的菌悬液处理(T0.50)促生效应显著,在甘薯茎叶封垄期的单株块根鲜质量和有效薯块数量最高.YTB1407通过提高ZR、t-ZR含量,降低IAAO活性、提高PPO活性和IAA含量以及(t-ZR+ZR)/IAA,促进块根分化建成初期甘薯不定根幼根向块根的分化;通过降低IAAO、PPO活性和提高IAA含量,降低(t-ZR+ZR)/IAA,促进块根分化建成后期甘薯分化根向块根的建成.

Abstract: We isolated the endophytic Bacillus amyloliquefaciens YTB1407 from the root of Panax quinquefolium, which has both biological control and growth promoting effects. To investigate its potential applications, a pot experiment of sweet potato was tested to assess the capacity of endophytic colonization of YTB1407 and the selection of its optimum concentration by investigating the performance of root characteristics on three time points in the whole early growth phase after irrigating with different concentrations of bacterial suspensions with treatment of sterile water as control. The activities of endogenous hormone IAA, ZR, t-ZR and IAA oxidase (IAAO, PPO, POD) were analyzed. The results showed that YTB1407 promoted the specific colonization of root system, the elongation of adventitious root and branch roots, and root activity in the early growth stage of sweet potato. At later growth stage, it formed greater fresh mass of absorption root and lower aboveground/root system mass ratio. YTB1407 suspensions with OD600 of 0.50 (T0.50) had more significant effect, which induced the highest fresh tuber mass and the largest effective tuber numbers of per plant at top cover stage. YTB1407 promoted the differentiation of adventitious roots into tubers at initial point of tuberization by increasing IAA content and the ratio of (t-ZR+ZR)/IAA, decreasing IAAO activity and enhancing PPO activity. Moreover, it promoted the differentiated roots into tubers at tuberization stage by keeping the higher content of IAA, lower ratio of (t-ZR+ZR)/IAA, and decreasing IAAO and PPO activities.