无机碳供应与光照条件对坛紫菜光合功能的影响

doi:10.13287/j.1001-9332.201802.010

应用生态学报 ›› 2018, Vol. 29 ›› Issue (2): 515-521.doi: 10.13287/j.1001-9332.201802.010

无机碳供应与光照条件对坛紫菜光合功能的影响

姜恒^1,2, 邹定辉^1*, 娄文勇²

¹华南理工大学环境与能源学院, 广州 510006;
²华南理工大学食品科学与工程学院, 广州 510006

收稿日期:2017-07-17 出版日期:2018-02-18 发布日期:2018-02-18
通讯作者: E-mail: dhzou@scut.edu.cn.
作者简介:姜恒, 男, 1988年生, 助理研究员. 主要从事海藻光合生理与环境生态研究. E-mail: jiangh@scut.edu.cn
基金资助:
本文由广东省科技计划项目(2015A020216004)和国家自然科学基金项目(41276148)资助

Effects of inorganic carbon supplies and light on photosynthetic functions of Pyropia haitanensis.

JIANG Heng^1,2, ZOU Ding-hui^1*, LOU Wen-yong²

¹School of Environment and Energy, South China University of Technology, Guangzhou 510006, China;
²School of Food Science and Engineering, South China University of Technology, Guangzhou 510006, China

Received:2017-07-17 Online:2018-02-18 Published:2018-02-18
Contact: E-mail: dhzou@scut.edu.cn.
Supported by:
This work was supported by the Science and Technology Planning Project of Guangdong Province (2015A020216004) and the National Natural Science Foundation of China (41276148).

摘要/Abstract

摘要： 海藻群体密度过大常引起海水中CO₂供应和光照强度降低,为探讨这两种环境条件对坛紫菜光合作用的影响,在4种条件下培养坛紫菜,即390 μL·L^-1(正常空气)+全日光、20 μL·L^-1(低CO₂供应)+全日光、390 μL·L^-1+低日光(光照强度为全日光的20%)、20 μL·L^-1+低日光,测定藻体的碳酸酐酶活性、光合速率,以及不同温度下开放状态光系统Ⅱ最大量子产量(F_v′/F_m′).结果表明: 低CO₂供应和低日光下生长的坛紫菜具有较高的碳酸酐酶活性,并且低日光能够提高海藻最大碳饱和光合放氧速率(V_max).在低日光下,生长在低CO₂海水中的坛紫菜V_max显著低于正常CO₂海水中生长的海藻V_max;在全日光下,低CO₂下生长的海藻V_max却高于正常CO₂下生长的海藻V_max.生长在低CO₂和低日光条件下的海藻在低温(10 ℃)和高温(30 ℃)下,藻体的F_v′/F_m′变化不明显;而生长在390 μL·L^-1+全日光环境中的海藻在高温环境下200 min后,藻体的F_v′/F_m′相比40 min时的F_v′/F_m′降低76.4%.低CO₂和低日光提高坛紫菜光合无机碳利用能力以及适应短期温度变化的能力,而低CO₂对海藻光合速率的影响与海藻所处光照环境有关.

Abstract: The increasing stocking density of algal often results in lowered carbon and light levels. In this study, Pyropia haitanensis were cultured under four different conditions, i.e., 390 μL·L^-1 (ambient carbon supply) + ambient sunlight, 20 μL·L^-1(decreased carbon supply) + ambient sunlight, 390 μL·L^-1 + decreased sunlight (20% of ambient sunlight), 20 μL·L^-1 + decreased sunlight. The activity of carbonic anhydrase (CA), photosynthetic rate and quantum efficiency of open photosynthetic system Ⅱ (F_v′/F_m′) of the algae under different temperatures were measured to investigate the effects of decreased carbon supply and lowered light on photosynthetic functions of P. haitanensis. The results showed the CA activities of the algae at decreased carbon supply and lowered sunlight was increased. The maximum C_i-saturated photosynthetic rates (V_max) were elevated under the low sunlight compared with that under the ambient sunlight. The V_max of the algae at low carbon seawater was lower than that at ambient carbon seawater under lowered sunlight. Under ambient sunlight, the V_max of the decreased carbon-grown algae was higher than that of ambient carbon-grown algae. The F_v′/F_m′ values of the algae grown at lowered carbon supply and decreased sunlight conditions showed no variation at low temperature (10 ℃) and high temperature (30 ℃), whereas it was decreased by about 76.4% when cultured at 390 μL·L^-1 + ambient sunlight at 200 min than that at 40 min under 30 ℃. These results suggested that decreased carbon supply and lowered sunlight intensity would improve in the capacity of HCO₃^- utilization and resistance to short-term temperature change in P. haitaneisis. The effects of low carbon on photosynthetic rate of the algae were dependent on light context.

姜恒, 邹定辉, 娄文勇. 无机碳供应与光照条件对坛紫菜光合功能的影响[J]. 应用生态学报, 2018, 29(2): 515-521.

JIANG Heng, ZOU Ding-hui, LOU Wen-yong. Effects of inorganic carbon supplies and light on photosynthetic functions of Pyropia haitanensis.[J]. Chinese Journal of Applied Ecology, 2018, 29(2): 515-521.

参考文献

[1] Friedlander M, Levy I. Cultivation of Gracilaria in outdoor tanks and ponds. Journal of Applied Phycology, 1995, 7: 315-324
[2] Israel A, Friedlander M. Inorganic carbon utilisation and growth abilities in the marine red macroalga Gelidiopsis sp. Israel Journal of Plant Sciences, 1998, 46: 117-124
[3] Richards DK, Hurd CL, Pritchard DW, et al. Photosynthetic response of monospecific macroalgal stands to density. Aquatic Biology, 2011, 13: 41-49
[4] Zou DH. The effects of severe carbon limitation on the green seaweed, Ulva conglobata (Chlorophyta). Journal of Applied Phycology, 2014, 26: 2417-2424
[5] Zou DH, Gao KS. Physiological responses of seaweeds to elevated atmospheric CO₂ concentrations// Seckbach J, Einav R, Israel A, eds. Seaweeds and Their Role in Globally Changing Environment. Berlin: Springer-Verlag, 2010
[6] Harley CDG, Anderson KM, Demes KW, et al. Effects of climate change on global seaweed communities. Journal of Phycology, 2012, 48: 1064-1078
[7] Koch M, Bowes G, Ross C, et al. Climate change and ocean acidification effects on seagrasses and marine macroalgae. Global Change Biology, 2013, 19: 103-132
[8] Ji Y, Xu ZG, Zou DH, et al. Ecophysiological responses of marine macroalgae to climate change factors. Journal of Applied Phycology, 2016, 28: 2953-2967
[9] Yao C-L (姚翠鸾), Somero GN. The impact of ocean warming on marine organisms. Chinese Science Bulletin (科学通报), 2015, 60(9): 805-816 (in Chinese)
[10] Sun J (孙军), Xue B (薛冰). Marine phytoplankton diversity and the impact of global climate change. Biodiversity Science (生物多样性), 2016, 24(7): 739-747 (in Chinese)
[11] Han Q-Y (韩秋影), Yin X-B (尹相博), Liu D-Y (刘东艳). Distribution of macroalgal community and environmental effects in Yangma Island, Yantai,Shandong Province, China. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(12): 3655-3663 (in Chinese)
[12] Andría JR, Brun FG, Pérez-Lloréns JL, et al. Acclimation responses of Gracilaria sp. (Rhodophyta) and Enteromorpha intestinalis (Chlorophyta) to changes in the external inorganic carbon concentration. Botanica Marina, 2001, 44: 361-370
[13] García-Sânchez MJ, Fernândez JA, Niell FX. Effect of inorganic carbon supply on the photosynthetic physiology of Gracilaria tenuistipitata. Planta, 1994, 194: 55-61
[14] Mercado JM, Javier F, Gordillo L, et al. Effects of different levels of CO₂ on photosynthesis and cell components of the red alga Porphyra leucosticte. Journal of Applied Phycology, 1999, 11: 455-461
[15] Zou DH, Gao KS, Xia JR. Photosynthetic utilization of inorganic carbon in the economic brown alga, Hizikia fusiforme (Sargassaceae) from the South China Sea. Journal of Phycology, 2003, 39: 1095-1100
[16] Jiang H, Zou DH, Li XH. Growth, photosynthesis and nutrient uptake by Grateloupia livida (Halymeniales, Rhodophyta) in response to different carbon levels. Phycologia, 2016, 55: 462-468
[17] Jiang H, Zou DH, Chen BB. Effects of lowered carbon supplies on two farmed red seaweeds, Pyropia haitanensis (Bangiales) and Gracilaria lemaneiformis (Gracilariales), grown under different sunlight conditions. Journal of Applied Phycology, 2016, 28: 3469-3477
[18] Zou DH, Gao KS. Effects of elevated CO₂ on the red seaweed Gracilaria lemaneiformis (Gigartinales, Rhodophyta) grown at different irradiance levels. Phycologia, 2009, 48: 510-517
[19] Lopes PF, Oliveira MC, Colepicolo P. Diurnal fluctuation of nitrate reductase activity in the marine red alga Gracilaria tenuistpitata (Rhodophyta). Journal of Phycology, 1997, 33: 225-231
[20] Talarico L, Maranzana G. Light and adaptive responses in red macroalgae: An overview. Journal of Photochemistry and Photobiology B: Biology, 2000, 56: 1-11
[21] Davison RI. Environmental effects on algal photosynthesis: Temperature. Journal of Phycology, 1991, 27: 2-8
[22] Zou DH, Gao KS. Photosynthetic bicarbonate utilization in Porphyra haitanensis (Bangiales, Rhodophyta). Chinese Science Bulletin, 2002, 47: 1629-1633
[23] Zou DH, Gao KS, Xia JR. Photosynthetic utilization of inorganic carbon in the economic brown alga, Hizikia fusiforme (Sargassaceae) from the South China Sea. Journal of Phycology, 2003, 39: 1095-1100
[24] Zou DH, Xia JR, Yang YF. Photosynthetic use of exogenous inorganic carbon in the agarophyte Gracilaria lemaneiformis (Rhodophyta). Aquaculture, 2004, 237: 421-431
[25] Invers O, Zimmerman RC, Alberte RS, et al. Inorganic carbon sources for seagrass photosynthesis: An experimental evaluation of bicarbonate use in species inhabiting temperate waters. Journal of Experimental Marine Biology and Ecology, 2001, 265: 203-217
[26] Ralph PJ, Gademann R, Larkum AWD, et al. In situ underwater measurements of photosynthetic activity of coral zooxanthellae and other reef-dwelling dinoflagellate endosymbionts. Marine Ecology Progress Series, 1999, 180: 139-147
[27] Jiang H, Zou DH, Lou WY, et al. Effects of stocking density and decreased carbon supply on the growth and photosynthesis in the farmed seaweed, Pyropia haitanensis (Bangiales, Rhodophyta). Journal of Applied Phycology, 2017, 29: 3057-3065
[28] Durchan M, Vacha F, Krieger-Liszkay A. Effects of severe CO₂ starvation on the photosynthetic electron transport chain in tobacco plants. Photosynthesis Research, 2001, 68: 203-213
[29] Talarico L, Maranzana G. Light and adaptive responses in red macroalgae: An overview. Journal of Photochemistry and Photobiology B: Biology, 2000, 56: 1-11
[30] Falkowski PG, Raven JA. Aquatic Photosynthesis. Malden: Blackwell Science, 1997
[31] Taiz L, Zeiger ET. Trans. Wang X-L (王学路), et al. Plant Physiology. Beijing: Science Press, 2009 (in Chinese)
[32] Kübler JE, Davison IR. Thermal acclimation of light use characteristics of Chondrus crispus (Rhodophyta). European Journal of Phycology, 1995, 30: 189-195
[33] Machalek KM, Davison IR, Falkowski PG. Thermal acclimation and photoacclimation of photosynthesis in the brown alga Laminaria saccharina. Plant, Cell and Environment, 1996, 19: 1005-1016
[34] Zou DH, Gao KS. The photosynthetic and respiratory responses to temperature and nitrogen supply in the marine green macroalga Ulva conglobata (Chlorophyta). Phycologia, 2014, 53: 86-94
[35] Zhang S-Y (章守宇), Liang J (梁君), Wang Z-H (汪振华), et al. Distribution characteristics of benthic algae in intertidal zone of Ma’an Archipelago of Zhejiang Province. Chinese Journal of Applied Ecology (应用生态学报), 2008, 19(10): 2299-2307 (in Chinese)
[36] Larcher S. Photosynthesis as a tool for indicating temperature stress events// Schulze ED, Caldwell MM, eds. Ecophysiology of Photosynthesis. Berlin: Springer-Verlag, 1994
[37] Wu B-G (武宝玕), Han Z-G (韩志国), Zang R-B (藏汝波). Effects of heat stress in marine red and green algae by chlorophyll fluorescence method. Journal of Jinan University (Natural Science) (暨南大学学报: 自然科学版), 2002, 23(1): 108-112 (in Chinese)

无机碳供应与光照条件对坛紫菜光合功能的影响

Effects of inorganic carbon supplies and light on photosynthetic functions of Pyropia haitanensis.

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