贵州茂兰喀斯特森林不同小生境下土壤细菌群落特征

doi:10.13287/j.1001-9332.201901.014

应用生态学报 ›› 2019, Vol. 30 ›› Issue (1): 108-116.doi: 10.13287/j.1001-9332.201901.014

贵州茂兰喀斯特森林不同小生境下土壤细菌群落特征

吴求生¹,龙健^1*,廖洪凯¹,刘灵飞¹,李娟²,吴劲楠¹,肖雄¹

¹贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室, 贵阳 550001;
²贵州师范大学地理与环境科学学院, 贵阳 550001

收稿日期:2018-04-16 修回日期:2018-09-19 出版日期:2019-01-20 发布日期:2019-01-20
通讯作者: longjian22@163.com
作者简介:吴求生, 男, 1992年生, 硕士研究生. 主要从事土壤环境化学研究. E-mail: 565715597@qq.com
基金资助:
本文由国家自然科学基金项目(41661045,41461072,41601249)和黔科合平台人才项目[2018]5609资助

Soil bacterial community characteristics under different microhabitat types on Maolan karst forest, Guizhou, Southwest China

WU Qiu-sheng¹, LONG Jian^1*, LIAO Hong-kai¹, LIU Ling-fei¹, LI Juan², WU Jin-nan¹, XIAO Xiong¹

¹Key Laboratory for Information System of Mountai-nous Areas and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang 550001, China;
²Department of Geography and Environment Science, Guizhou Normal University, Guiyang 550001, China

Received:2018-04-16 Revised:2018-09-19 Online:2019-01-20 Published:2019-01-20
Supported by:
This work was supported by the National Natu-ral Science Foundation of China (41661045, 41461072, 41601249) and the Science and Technology Cooperation Plan of Guizhou Province ([2018]5609).2018-04-16 Received, 2018-09-19 Accepted.*

摘要/Abstract

摘要： 以贵州茂兰喀斯特森林小生境土壤(石洞、石缝、石沟、石槽、土面)为研究对象,利用高通量测序技术对其土壤细菌16S rRNA V3～V4可变区进行高通量测序,分析土壤细菌α多样性、物种组成及丰度差异情况,并应用数量生态学方法分析土壤理化因子对细菌群落结构的影响.结果表明: 5类小生境土壤共检测到27个门64个纲128个目242个科367个属704个种;主要优势菌门为变形菌门、放线菌门、酸杆菌门、绿弯菌门、硝化螺旋菌门;石洞表层(0～10 cm)、石洞下层(10～20 cm)、石缝及石槽等生境主要的优势菌门是放线菌门,土面表层(0～10 cm)、土面下层(10～20 cm)的是酸杆菌门,石沟表层(0～10 cm)、石沟下层(10～20 cm)的是变形菌门.石缝细菌Simpson多样性最高,土面下层(10～20 cm)最低.LEfSe分析表明,土面表层(0～10 cm)、土面下层(10～20 cm)、石洞下层(10～20 cm)以及石槽不同分类水平上的差异指示种数量高于石缝、石沟表层(0～10 cm)以及石沟下层(10～20 cm).例如,在门水平上,石洞下层(10～20 cm)差异指示种为放线菌门与绿弯菌门,石沟表层(0～10 cm)为变形菌门与Tectomicrobia门,土面表层(0～10 cm)为酸杆菌门、疣微菌门及Latescibacteria门,土面下层(10～20 cm)为硝化螺旋菌门,石槽、石缝以及石沟下层(10～20 cm)无差异指示种;但从门至属,石洞表层(0～10 cm)都无差异指示种.冗余分析(RDA)及集成推进树(ABT)分析表明,土壤有机质、pH及总磷较大程度上解释了细菌门类水平分布对土壤基本理化因子变化的响应机制.

关键词: 集成推进树, 群落特征, 茂兰喀斯特, 土壤细菌, 小生境

Abstract: We analyzed the diversity, community composition and relative abundance of soil bacteria communities in five different types of microhabitats (included stone caverns, stone crevices, stone gullies, stone grooves, top-soils) in Maolan karst forest, Guizhou Province, China. Bacterial 16S rRNA V3-V4 variable regions were analyzed by high-throughput sequencing technique. We examined the effects of soil physicochemical factors on bacterial community structure. The results showed that 27 phyla, 64 classes, 128 orders, 242 families, 367 genera, and 704 species were detected in the soils from five types of microhabitats. Proteobacteria, Actinomycetes, Acidobacteria, Chlorofleixi and Nitrospirae were the dominant bacterial phyla. Actinomycetes was the dominant phyla in the microhabitats of top-stone cavern (0-10 cm), sub-stone cavern (10-20 cm), stone crevice, and stone groove. Acidobacteria was the dominant phyla in the top-soil (0-10 cm) and the sub-soil (10-20 cm). Proteobacteria was the dominant phyla in the top-stone gully (0-10 cm) and the sub-stone gully (10-20 cm). The highest soil bacterial Simpson index was found in stone crevice microhabitat, while the lowest was found in sub-soil (10-20 cm) microhabitat. Results from the LEfSe analysis showed that the number of distinct indicator species at the different levels of taxo-nomy was higher in the top-soil (0-10 cm), sub-soil (10-20 cm), sub-stone cavern (10-20 cm), and the stone groove than in the stone crevice, the top-stone gully (0-10 cm) and sub-stone gully (10-20 cm). The indicator phyla were Actinobacteria and Chlorofleixi in the sub-stone cavern (10-20 cm). The top-stone gully (0-10 cm) were characterized by the phyla of Proteobacteria and Tectomicrobia. The top-soil (0-10 cm) was featured by Acidobacteria, Verrucomicrobia and Latescibacteria. The sub-soil (10-20 cm) was dominated by Nitrospirae. There were no indicator species in stone groove, stone crevice, and sub-stone gully (10-20 cm). There was no indicator species in phylum to genus in top-stone cavern (0-10 cm). RDA and ABT analysis showed that soil organic matter, pH, and available phosphorus explained a large part of the variation regarding the responses of bacterial community to the changes in basic physicochemical factors in the soil.

Key words: aggregated boosted tree., community characteristics, Maolan karst, soil bacteria, microhabitat

吴求生,龙健,廖洪凯,刘灵飞,李娟,吴劲楠,肖雄. 贵州茂兰喀斯特森林不同小生境下土壤细菌群落特征[J]. 应用生态学报, 2019, 30(1): 108-116.

WU Qiu-sheng, LONG Jian, LIAO Hong-kai, LIU Ling-fei, LI Juan, WU Jin-nan, XIAO Xiong. Soil bacterial community characteristics under different microhabitat types on Maolan karst forest, Guizhou, Southwest China[J]. Chinese Journal of Applied Ecology, 2019, 30(1): 108-116.

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贵州茂兰喀斯特森林不同小生境下土壤细菌群落特征

Soil bacterial community characteristics under different microhabitat types on Maolan karst forest, Guizhou, Southwest China

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