有机污染土壤电动修复过程中微生物的分布特征及模型构建

doi:10.13287/j.1001-9332.201911.033

应用生态学报 ›› 2019, Vol. 30 ›› Issue (11): 3921-3930.doi: 10.13287/j.1001-9332.201911.033

有机污染土壤电动修复过程中微生物的分布特征及模型构建

鲁文杰^1,2,3, 郭书海^1,2*, 程凤莲^1,2, 李玉华⁴, 王卅^1,2, 吴波^1,2

¹中国科学院沈阳应用生态研究所, 沈阳 110016;
²污染土壤生物-物化协同修复技术国家地方联合工程实验室, 沈阳 110016;
³中国科学院大学, 北京 100049;
⁴辽宁省国家新型原材料基地建设工程中心, 沈阳 110032

收稿日期:2019-03-13 出版日期:2019-11-15 发布日期:2019-11-15
通讯作者: * E-mail: shuhaiguo@iae.ac.cn
作者简介:鲁文杰, 男, 1993年生, 硕士研究生. 主要从事有机污染土壤电动微生物修复研究. E-mail:1252468922@ qq.com
基金资助:
本文由国家重点研发计划重点专项(2018YFC1801900)、辽宁省“兴辽英才计划”项目(XLYC1802111)和国家自然科学基金项目(21677150)资助

Distribution characteristics and model establishment of microorganisms in electrokinetic remediation of organic contaminated soil.

LU Wen-jie^1,2,3, GUO Shu-hai^1,2*, CHENG Feng-lian^1,2, LI Yu-hua⁴, WANG Sa^1,2, WU Bo^1,2

¹Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
²National-Local Joint Engineering Laboratory of Contaminated Soil Remediation by Bio-physicochemical Synergistic Process, Shenyang 110016, China;
³University of Chinese Academy of Sciences, Beijing 100049, China;
⁴Liaoning Province National New Raw Material Base Construction Engineering Center, Shenyang 110032, China

Received:2019-03-13 Online:2019-11-15 Published:2019-11-15
Contact: * E-mail: shuhaiguo@iae.ac.cn
Supported by:
This work was supported by the Key Project of National Key Research and Development Program of China (2018YFC1801900), Liaoning Revitalization Talent Program (XLYC1802111), and National Natural Science Foundation of China (21677150).

摘要/Abstract

摘要： 外加电场下土壤微生物会发生快速繁殖和定向迁移.本研究在十四烷污染土壤中不同位置投加十四烷高效降解菌,并施加1 V·cm^-1的单向直流电场,考察目标菌群的迁移分布及降解特征.结果表明:微生物受电渗析和电泳作用分别向阴极和阳极迁移,电渗析迁移量是电泳的3.5倍.同时,施加电场还会使土壤环境在空间上存在差异进而影响微生物生长,施加电场的土壤中微生物数量平均值为1.16×10⁸ CFU·g^-1(6 d),是不施加电场处理组的2.3倍;S₂～S₄区是微生物的高效生长区域,电动30 d后,区域平均数量是阴阳极的2.8～3.5倍,是对照处理组的2.1倍.十四烷降解率与微生物数量呈显著正相关关系(r=0.895, P<0.05),最佳降解区域在近阴极区(S₄),可达94.6%.基于试验结果模拟,建立了环境因子修正的电动区域微生物分布模型.该模型结合电动激活和电动运移作用对土壤微生物的叠加影响,实现了定点投加微生物在电动过程中数量的分布模拟.研究结果可为外源功能菌在电动-微生物修复有机污染土壤中的高效引入提供理论依据.

Abstract: Soil microorganisms would reproduce rapidly and migrate directionally under the applied electric field. In this study, tetradecane-degrading bacteria were added at different locations in soil, and 1 V·cm^-1 unidirectional DC electric field was applied to investigate the migration, distribution, and degradation characteristics of target bacteria in soil. The results showed that microorga-nisms migrated to the cathode and anode by electrodialysis and electrophoresis, respectively. The number of microorganisms migrated by electrodialysis was 3.5 times of that by electrophoresis. The application of electric field could make the soil environment spatially heterogeneous to affect the growth of microorganisms. The average number of soil microorganisms under electric field was 1.16×10⁸ CFU·g^-1(6 days), which was 2.3 times of that without electric field. S₂ to S₄ were the efficient growth areas of microorganisms, with the average number after 30-day electric treatment being 2.8-3.5 times of that on the anode and cathode, and 2.1 times of that in the control. There was a significant positive correlation between the tetradecane degradation rate and the number of microorganisms (r=0.895, P<0.05). The optimal tetradecane degradation region was near the cathode (S₄), where its degradation rate reached 94.6%. Based on these results, a model modified by environmental factors was established to simulate the spatial distribution of microorganisms under the combined effect of electrokinetic stimulation and transportation. According to the model, the number distribution of microorganisms could be deduced when exogenous bacteria were added in a fixed section. The results provide a theoretical basis for the efficient import of exogenous functional bacteria in electrokinetic-bioremediation of organic contaminated soil.

鲁文杰, 郭书海, 程凤莲, 李玉华, 王卅, 吴波. 有机污染土壤电动修复过程中微生物的分布特征及模型构建[J]. 应用生态学报, 2019, 30(11): 3921-3930.

LU Wen-jie, GUO Shu-hai, CHENG Feng-lian, LI Yu-hua, WANG Sa, WU Bo. Distribution characteristics and model establishment of microorganisms in electrokinetic remediation of organic contaminated soil.[J]. Chinese Journal of Applied Ecology, 2019, 30(11): 3921-3930.

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有机污染土壤电动修复过程中微生物的分布特征及模型构建

Distribution characteristics and model establishment of microorganisms in electrokinetic remediation of organic contaminated soil.

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