西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪的抗性风险和抗性稳定性

doi:10.13287/j.1001-9332.202010.035

应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3289-3295.doi: 10.13287/j.1001-9332.202010.035

西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪的抗性风险和抗性稳定性

颜改兰¹, 王圣印^2*

¹陕西省西咸新区市场监督管理局, 西安 710086;
²浙江农林大学农业与食品科学学院, 杭州 311300

收稿日期:2020-01-28 接受日期:2020-07-14 出版日期:2020-10-15 发布日期:2021-04-15
通讯作者: * E-mail: wsy19840822@163.com
作者简介:颜改兰, 女, 1989年生. 主要从事药学和昆虫毒理学研究. E-mail: 319915187@qq.com
基金资助:
农业部西北黄土高原有害生物综合治理重点实验室开放性基金项目(KFJJ20180109)资助

Resistance risk and resistance stability of Frankliniella occidentalis to nitenpyram, clothianidin and thiamethoxam

YAN Gai-lan¹, WANG Sheng-yin^2*

¹Xi-Xian New District Administration for Market Regulation, Xi'an 710086, China;
²College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, China

Received:2020-01-28 Accepted:2020-07-14 Online:2020-10-15 Published:2021-04-15
Contact: * E-mail: wsy19840822@163.com
Supported by:
Open Fund Project of the Key Laboratory of Comprehensive Harmful Pest Management of the Northwest Loess Plateau of the Ministry of Agriculture (KFJJ20180109).

摘要/Abstract

摘要： 为明确使用新烟碱类杀虫剂烯啶虫胺、噻虫胺和噻虫嗪防治入侵害虫西花蓟马的抗性风险及抗性稳定性,本研究采用芸豆浸药法对西花蓟马敏感种群初羽化雌成虫进行连续筛选获得抗性种群,根据抗性现实遗传力计算公式分析西花蓟马对上述3种杀虫剂的抗性风险,预测其抗性发展速度,并测定抗性稳定性。结果表明: 经过30代抗性筛选,西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪均达到高水平抗性(44.7、45.5和32.7倍)。西花蓟马对噻虫胺、烯啶虫胺和噻虫嗪的抗性发展速度依次降低,抗性现实遗传力分别为0.1503、0.1336和0.1258。对抗性种群在无选择压力下继续饲养10代,西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪的抗性水平均出现一定程度的下降,但均未能恢复到敏感性水平。抗性选育后,西花蓟马若虫与成虫对杀虫剂的敏感性差异显著缩小,西花蓟马敏感种群及抗性种群若虫对上述3种杀虫剂的敏感性显著高于成虫。西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪均存在高抗风险,噻虫嗪的抗性上升速度较慢且抗性稳定性最低。因此,在西花蓟马若虫期使用噻虫嗪有利于西花蓟马防治。

关键词: 西花蓟马, 烯啶虫胺, 噻虫胺, 噻虫嗪, 抗性风险, 抗性稳定性

Abstract: To effectively control the damage of Frankliniella occidentalis (Pergande), we evalutated the resistance risk and resistance stability of F. occidentalis to nitenpyram, clothianidin and thiamethoxam. With the method of dipping Phaseolus vuglaris, we selected the resistance populations from the susceptible population with nitenpyram, clothianidin and thiamethoxam, respectively. Both the resistance inheritance and resistance risk were analyzed with the resistance reality hereditary. After 30 generations' selections, the selected-populations showed high level of insecticide resistance to nitenpyram, clothianidin and thiamethoxam, with a resistance ratio of 44.7-fold, 45.5-fold, and 32.7-fold, respectively. The development rate of resistance to clothianidin, nitenpyram, and thiamethoxam reduced in turn, with a resistance reality heritability of 0.1503, 0.1336 and 0.1258, respectively. Stopping selection for 10 continuously generations, the resistance levels of selection resis-tance populations declined slowly, but could not regain the original susceptibility to nitenpyram, clothianidin and thiamethoxam. After resistance selection, the sensitivity of F. occidentalis nymphs to clothianidin, nitenpyram, and thiamethoxam was significantly higher than that of adults. F. occidentalis had the great potential to gain high level resistance to nitenpyram, clothianidin and thiamethoxam. Compared with other two insecticides, the resistance of F. occidentalis to thiamethoxam increased slower and decreased faster. Therefore, using thiamethoxam in nymph stage might be beneficial to effectively control F. occidentalis.

Key words: Frankliniella occidentalis, nitenpyram, clothianidin, thiamethoxam, resistance risk, resistance stability

颜改兰, 王圣印. 西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪的抗性风险和抗性稳定性[J]. 应用生态学报, 2020, 31(10): 3289-3295.

YAN Gai-lan, WANG Sheng-yin. Resistance risk and resistance stability of Frankliniella occidentalis to nitenpyram, clothianidin and thiamethoxam[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3289-3295.

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西花蓟马对烯啶虫胺、噻虫胺和噻虫嗪的抗性风险和抗性稳定性

Resistance risk and resistance stability of Frankliniella occidentalis to nitenpyram, clothianidin and thiamethoxam

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