Abstract: The early-spring and autumn-winter tomato in greenhouse is the main planting patterns of protected vegetable in North China. Taking the customary fertilization amount of farmers under this planting pattern as control (CK, 100% chemical fertilizer), six organic fertilizer replacing chemical fertilizer treatments were set, namely, 15% (T₁), 30% (T₂), 45% (T₃), 60% (T₄), 75% (T₅), 100% organic fertilizer (T₆), to examine the replacing effects on soil environment and tomato yield. The results showed that soil bacteria increased with increasing organic fertilizer amount, the actinomycetes wers up to 12.12×10⁶ cfu·g^-1 under T₁ treatment which was the highest one. Combined application of organic fertilizer could increase soil urease activity, decrease catalase activity, increase soil organic matter, and enhance the concentrations of available phosphorus and available potassium, indicating that organic fertilizer had significant effects on soil physical and chemical properties, soil enzyme activity and nutrient accumulation. The invertase activity and available phosphorus content under T₁ treatment were the highest, being 1.36 mg·g^-1 and 305.4 mg·kg^-1, respectively, while the available potassium content of T₂ treatment was the highest (582.6 g·kg^-1). In addition, T₂ had the largest percentage of >0.25 mm water-stable agglomerate (94.2%). Compared with CK, the application of organic fertilizer could improve tomato quality and increase yield. The lycopene content of T₁ was the highest (5.69), the sugar-acid ratio of T₁ and T₂ was 8.19 and 8.70, respectively, with better tastes. The yield of T₁ was the highest, followed by T₂ treatment, which was 16.6% and 5.8% higher than that of CK, respectively. It suggested that reducing the application rate of chemical fertilizers by 15%-30% with organic fertilizer was a preferred fertilization measure in this planting pattern.