宁夏枸杞对枸杞瘿螨为害的内源激素响应及外源水杨酸对枸杞瘿螨的影响

doi:10.13287/j.1001-9332.202006.039

应用生态学报 ›› 2020, Vol. 31 ›› Issue (7): 2307-2313.doi: 10.13287/j.1001-9332.202006.039

宁夏枸杞对枸杞瘿螨为害的内源激素响应及外源水杨酸对枸杞瘿螨的影响

杨孟可¹, 李建领^1,2, 刘赛¹, 乔海莉¹, 郭昆¹, 徐荣¹, 陈君¹, 徐常青^1*

¹中国医学科学院-北京协和医学院药用植物研究所, 北京 100193;
²华润三九医药股份有限公司, 广东深圳 518110

收稿日期:2019-11-24 接受日期:2020-03-21 出版日期:2020-07-15 发布日期:2021-01-15
通讯作者: E-mail: cqxu@implad.ac.cn
作者简介:杨孟可,女,1995年生,硕士研究生。主要从事药用植物病虫害研究。E-mail: mengke95@163.com
基金资助:
国家自然科学基金项目(81673699,81274198)、中国医学科学院医学与健康科技创新工程项目(CIFMS 2016-I2M-3-017)和国家重点研发计划项目(2017YFC1700706)资助

Hormone response of Lycium barbarum to gall mite Aceria pallida and effects of exogenous salicylic acid on the infestation and development of gall mite

YANG Meng-ke¹, LI Jian-ling^1,2, LIU Sai¹, QIAO Hai-li¹, GUO Kun¹, XU Rong¹, CHEN Jun¹, XU Chang-qing^1*

¹Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China;
²China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shen-zhen 518110, Guangdong, China

Received:2019-11-24 Accepted:2020-03-21 Online:2020-07-15 Published:2021-01-15
Contact: E-mail: cqxu@implad.ac.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (81673699, 81274198), the Chinese Academy of Medical Sciences Innovation Fund for Medical Science (CIFMS 2016-I2M-3-017) and the National Key Research and Development Program of China (2017YFC1700706).

摘要/Abstract

摘要： 为探索宁夏枸杞对枸杞瘿螨致瘿过程诱导的激素响应及外源水杨酸对枸杞瘿螨的影响,利用LC-MS/MS技术对枸杞瘿螨为害后宁夏枸杞叶片中水杨酸(SA)、茉莉酸(JA)、生长素(Auxin)和脱落酸(ABA)4种内源激素进行测定,并通过浸叶法研究外源SA对枸杞瘿螨侵染过程、虫瘿生长和种群增长的影响。结果表明: 枸杞瘿螨的致瘿行为可引发枸杞叶片中多种内源激素产生变化,其中SA和JA的变化最显著,分别是对照组含量的4.0和13.0倍,而Auxin和ABA的变化不显著;外源SA对枸杞瘿螨虫瘿生长有显著抑制作用,且随时间的延长,抑制作用增强,同时虫瘿内瘿螨种群数量受到显著抑制。枸杞瘿螨能够同时诱导枸杞产生SA和JA抗性,外源SA对枸杞瘿螨虫瘿生长和瘿螨种群数量均具有显著抑制作用,利用外源SA防治枸杞瘿螨具有一定的应用前景。

关键词: 植物防御, 诱导抗性, 植物激素, 侵染, 发育

Abstract: To investigate the hormone responses of Lycium barbarum to Aceria pallida infestation and the effects of exogenous salicylic acid on the infestation and development of gall mite, four phytohormones (salicylic acid (SA), jasmonic acid (JA), Auxin, and abscisic acid (ABA)) of L. barbarum leaves infested with A. pallida were determined by LC-MS/MS. Moreover, the effects of exogenous SA on the growth of gall and mite population were investigated. The results showed that contents of SA and JA in leaves infested with A. pallida were significantly higher (4.0 and 13.0 folds, respectively) than that in control, but that of Auxin and ABA did not change. The growth of gall was inhibited significantly by exogenous SA, with the inhibition increasing with the prolongation of treatment time. Meanwhile, mite population in galls treated with SA was significantly lower than that in control. Our results suggested that A. pallida could induce SA and JA resistance of L. barbarum, and that the exogenous SA could inhibit the growth of gall and mite population. Exogenous SA could be potentially used in A. pallida prevention.

Key words: plant defense, induced resistance, plant hormone, infestation, development

杨孟可, 李建领, 刘赛, 乔海莉, 郭昆, 徐荣, 陈君, 徐常青. 宁夏枸杞对枸杞瘿螨为害的内源激素响应及外源水杨酸对枸杞瘿螨的影响[J]. 应用生态学报, 2020, 31(7): 2307-2313.

YANG Meng-ke, LI Jian-ling, LIU Sai, QIAO Hai-li, GUO Kun, XU Rong, CHEN Jun, XU Chang-qing. Hormone response of Lycium barbarum to gall mite Aceria pallida and effects of exogenous salicylic acid on the infestation and development of gall mite[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2307-2313.

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宁夏枸杞对枸杞瘿螨为害的内源激素响应及外源水杨酸对枸杞瘿螨的影响

Hormone response of Lycium barbarum to gall mite Aceria pallida and effects of exogenous salicylic acid on the infestation and development of gall mite

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