不同形态微生物菌剂对不结球白菜生长和品质的影响

doi:10.13287/j.1001-9332.202105.021

应用生态学报 ›› 2021, Vol. 32 ›› Issue (5): 1777-1782.doi: 10.13287/j.1001-9332.202105.021

不同形态微生物菌剂对不结球白菜生长和品质的影响

温丹^1,2,3, 王晓¹, 孙凯宁¹, 王克安¹, 高建伟¹, 张伟², 杨宁^1*

¹山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心/农业农村部黄淮地区蔬菜科学观测实验站(山东), 济南 250100;
²山东大学生命科学学院, 山东青岛 266237;
³山东葆婴堂母婴教育有限公司, 山东济宁 272000

收稿日期:2020-10-21 接受日期:2021-02-09 出版日期:2021-05-15 发布日期:2021-11-15
通讯作者: *E-mail: 67169110@qq.com
作者简介:温丹,女,1988年生,博士,助理研究员。主要从事设施蔬菜栽培研究。E-mail:saaswd@126.com
基金资助:
山东省现代农业产业技术体系蔬菜创新团队项目(SDAIT-05-07)和山东省农业科学院农业科技创新工程项目(CXGC2016B06, CXGC2018E08)资助

Effects of different forms of microbial agents on the growth and quality of Brassica rapa L. ssp. chinensis Makino (non-heading Chinese cabbage).

WEN Dan^1,2,3, WANG Xiao¹, SUN Kai-ning¹, WANG Ke-an¹, GAO Jian-wei¹, ZHANG Wei², YANG Ning^1*

¹Vegetable and Flower Research Institute of Shandong Academy of Agricultural Sciences/Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Sub-Center/Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables (Shandong), Ministry of Agriculture and Rural Affairs, Ji'nan 250100, China;
²School of Life Science, Shandong University, Qingdao 266237, Shandong, China;
³Shandong Baoyingtang Maternal and Child Education Co. Ltd., Ji'ning 272000, Shandong, China

Received:2020-10-21 Accepted:2021-02-09 Online:2021-05-15 Published:2021-11-15
Contact: *E-mail: 67169110@qq.com
Supported by:
Agriculture Industrial Technology System Funding of Shandong Province of China (SDAIT-05-07) and the Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2016B06, CXGC2018E08).

摘要/Abstract

摘要： 合理施用微生物菌剂可显著改善蔬菜土壤环境,增加产量,提升品质。本试验以不结球白菜为供试材料,在设施条件下设置不施微生物菌、施用液态和固态微生物菌剂处理,研究不同形态微生物菌剂对不结球白菜生长和品质的影响。结果表明: 施用液态和固态微生物菌剂均能显著提高土壤脲酶活性,增加植株全氮含量,增大叶面积,显著提升叶片SPAD值,提高叶片净光合速率,产量比不施微生物菌剂处理分别增加26.9%和34.4%。同时,施用微生物菌剂可提高不结球白菜的总酚和抗坏血酸含量,显著降低硝酸盐含量。可见,合理施用微生物菌剂可促进不结球白菜产量的提升和品质的改善。不同形态微生物菌剂中,液态微生物菌剂时效快,固态微生物菌剂长效性好,可根据土壤肥力情况酌情配施有机肥或无机肥以促进蔬菜的优质高产。

关键词: 微生物菌剂, 不结球白菜, 生长, 品质

Abstract: Reasonable application of microbial agents can significantly improve soil environment and increase the yield and quality of vegetables. In this study, we examined the effects of different forms of microbial agents on the growth and quality of Brassica rapa L. ssp. chinensis Makino (non-heading Chinese cabbage) under the conditions of no microbial agent application, liquid and solid microbe agents application. The results showed that compared with no microbial agent, application of liquid and solid microbial agent significantly improved soil urease activity, plant nitrogen content, leaf area, SPAD value, and net photosynthetic rate, and increased vegetable production by 26.9% and 34.4% respectively. Meanwhile, the total phenol content and ascorbic acid content of non-heading Chinese cabbage were increased and nitrate content was significantly decreased by applying microbial agents. Thus, rational application of microbial agents promoted the yield and quality of non-heading Chinese cabbage. From the perspective of vegetable growth, liquid microbial agents work fast, solid microbial agents had good long-term efficacy. The cooperating application with organic and inorganic fertilizers would facilitate the excellent quality and high yield of vegetables.

Key words: microbial agents, non-heading Chinese cabbage, growth, quality

温丹, 王晓, 孙凯宁, 王克安, 高建伟, 张伟, 杨宁. 不同形态微生物菌剂对不结球白菜生长和品质的影响[J]. 应用生态学报, 2021, 32(5): 1777-1782.

WEN Dan, WANG Xiao, SUN Kai-ning, WANG Ke-an, GAO Jian-wei, ZHANG Wei, YANG Ning. Effects of different forms of microbial agents on the growth and quality of Brassica rapa L. ssp. chinensis Makino (non-heading Chinese cabbage).[J]. Chinese Journal of Applied Ecology, 2021, 32(5): 1777-1782.

参考文献

[1] 李天来, 许勇, 张金霞. 我国设施蔬菜、西甜瓜和食用菌产业发展的现状及趋势. 中国蔬菜, 2019(11): 6-9 [Li T-L, Xu Y, Zhang J-X. The present situation and trend of the development of facility vegetable, melon and edible fungus industry in China. China Vegetables, 2019(11): 6-9]
[2] 吴林坤, 吴红淼, 朱铨, 等. 不同改良措施对太子参根际土壤酚酸含量及特异菌群的影响. 应用生态学报, 2016, 27(11): 3623-3630 [Wu L-K, Wu H-M, Zhu S, et al. Effects of different amendments on contents of phenolic acids and specific microbes in rhizosphere of Pseudostellaria heterophylla. Chinese Journal of Applied Ecology, 2016, 27(11): 3623-3630]
[3] 王亚男, 曾希柏, 王玉忠, 等. 设施蔬菜种植年限对氮素循环微生物群落结构和丰度的影响. 应用生态学报, 2014, 25(4): 1115-1124 [Wang Y-N, Zeng X-B, Wang Y-Z, et al. Effects of vegetable cultivation years on microbial biodiversity and abundance of nitrogen cycling in greenhouse soils. Chinese Journal of Applied Ecology, 2014, 25(4): 1115-1124]
[4] Etesami H, Maheshwari DK. Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicology and Environmental Safety, 2018, 156: 225-246
[5] 郭月. 设施农业土壤质量退化问题及改良对策. 现代农业科技, 2017(17): 188, 191 [Guo Y. Soil quality degradation and its improvement in the facility agriculture. Modern Agricultural Science and Technology, 2017(17): 188, 191]
[6] 马建宏, 冒维维. 设施蔬菜连作障碍综合防控措施分析. 特种经济动植物, 2019, 22(11): 30-31 [Ma J-H, Mao W-W. Analysis of comprehensive prevention and control measures for continuous cropping obstacles of facility vegetables. Special Economic Animal and Plant, 2019, 22(11): 30-31]
[7] 刘一江, 都林娜, 康华靖. 微生物菌剂对水稻植株性状、产量及土壤理化性质的影响. 中国稻米, 2019, 25(6): 39-42 [Liu Y-J, Du L-N, Kang H-J. Effects of microbial inoculants on rice growth and soil physical and chemical properties. China Rice, 2019, 25(6): 39-42]
[8] 侯乐梅, 孟瑞青, 乜兰春, 等. 不同微生物菌剂对基质酶活性和番茄产量及品质的影响. 应用生态学报, 2016, 27(8): 2520-2526 [Hou L-M, Meng R-Q, Nie L-C, et al. Effects of different microbial agents on substrate enzyme activities and tomato yield and quality. Chinese Journal of Applied Ecology, 2016, 27(8): 2520-2526]
[9] 王强, 彭开松, 严兵, 等. 枯草芽孢杆菌源抗生素研究进展. 动物医学进展, 2010, 31(9): 97-101 [Wang Q, Peng K-S, Yan B, et al. Progress on antibio-tics of Bacillus subtilis source. Progress in Veterinary Medicine, 2010, 31(9): 97-101]
[10] 肖嫩群, 黄晓辉, 彭运祥, 等. 芽孢杆菌B13对白菜产量和品质的影响. 中国微生态学杂志, 2014, 26(1): 27-30 [Xiao N-Q, Huang X-H, Peng Y-X, et al. Effects of Bacillus B13 on yield and quality of Chinese cabbage. Chinese Journal of Microecology, 2014, 26(1): 27-30]
[11] Saxena AK, Kumar M, Chakdar H, et al. Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology, 2020, 128: 1583-1594
[12] 黄大野, 曹春霞, 张亚妮, 等. 枯草芽孢杆菌NBF809防治番茄棒孢叶斑病研究. 中国蔬菜, 2018(12): 40-44 [Huang D-Y, Cao C-X, Zhang Y-N, et al. Studies on controlling tomato Corynespora leaf spot by Bacillus subtilis NBF809. China Vegetables, 2018(12): 40-44]
[13] Franco-Sierra ND, Posada LF, Santa-María G, et al. Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture. Functional and Integrative Genomics, 2020, 20: 575-589
[14] 兰莹, 王雪, 胡贸英, 等. 枯草芽孢杆菌对寒地温室蔬菜病原菌的抑制研究. 生物灾害科学, 2017, 40(4): 263-267 [Lan Y, Wang X, Hu M-Y, et al. Inhibitory effects of Bacillus subtilis on plant pathogens of conservatory in high latitudes. Biological Disaster Science, 2017, 40(4): 263-267]
[15] 常文程, 刘彬, 陆鋆赟, 等. 100亿个/g枯草芽孢杆菌可湿性粉剂防治青菜根肿病效果研究. 上海蔬菜, 2019(4): 55-56 [Chang W-C, Liu B, Lu Y-Y, et al. Study on the effect of 10 billion/g of Bacillus subtilis wettable powder on the control of root swelling of green cabbage. Shanghai Vegetables, 2019(4): 55-56]
[16] Ramandeep KT, Geoffrey PS. Antioxidant activity in different fractions of tomatoes. Food Research International, 2005, 38: 487-494
[17] 刘长虹. 中短波紫外线调节采后番茄品质及相关次生代谢的效应与机理. 博士论文. 杭州: 浙江大学, 2012 [Liu C-H. Study on the Effects and Mechanism of UV-C or UV-B Treatment on Quality and Secondary Metabolism in Postharvest Tomato Fruit. PhD Thesis. Hangzhou: Zhejiang University, 2012]
[18] 华晶忠, 刘笑笑, 王丽, 等. 芦笋中维生素C含量测定及变化规律. 食品工业, 2016, 37(10): 281-283 [Hua J-Z, Liu X-X, Wang L, et al. Determination of vitamin C and its content changes in Asparagus. The Food Industry, 2016, 37(10): 281-283]
[19] Cataldo DA, Maroon M, Schrader LE, et al. Rapid colo-rimetric determination of nitrate in plant-tissue by nitration of salicylic-acid. Communications in Soil Science and Plant Analysis, 1975, 6: 71-80
[20] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000 [Bao S-D. Soil and Agricultural Chemistry Analysis. Beijing: China Agriculture Press, 2000]
[21] 关松荫. 土壤酶及其研究法. 北京: 农业出版社, 1986 [Guan S-Y. Soil Enzyme Research Methods. Beijing: China Agriculture Press, 1986]
[22] 李荣发, 刘鹏, 董树亭, 等. 肥料配施枯草芽孢杆菌对夏玉米产量及养分利用的影响. 植物营养与肥料学报, 2019, 25(9): 1607-1614 [Li R-F, Liu P, Dong S-T, et al. Effect of combined application of fertilizer and Bacillus subtilis on grain yield and fertilizer utilization efficiency of summer maize (Zea mays L.). Journal of Plant Nutrition and Fertilizers, 2019, 25(9): 1607-1614]
[23] 田露, 刘景辉, 赵宝平, 等. 保水剂和微生物菌肥配施对旱作燕麦干物质积累、分配、转运和产量的影响. 生态学杂志, 2020, 39(9): 2996-3003 [Tian L, Liu J-H, Zhao B-P, et al. Effects of combination of water-retaining agent and microbial fertilizer on dry matter accumulation, allocation, transportation, and yield of oat in rainfed farmland. Chinese Journal of Ecology, 2020, 39(9): 2996-3003]
[24] 许景钢, 孙涛, 李嵩. 我国微生物肥料的研发及其在农业生产中的应用. 作物杂志, 2016(1): 1-6 [Xu J-G, Sun T, Li S. Application of microbial fertilizers in agricultural production of China. Crops, 2016(1): 1-6]
[25] 范丙全. 我国生物肥料研究与应用进展. 植物营养与肥料学报, 2017, 23(6): 1602-1613 [Fan B-Q. Advances in biofertilizer research and development in China. Journal of Plant Nutrition and Fertilizers, 2017, 23(6): 1602-1613]
[26] 李忠, 江立庚, 唐荣华, 等. 连作对花生土壤酶活性、养分含量和植株产量的影响. 土壤, 2018, 50(3): 491-497 [Li Z, Jiang L-G, Tang R-H, et al. Effects of long-term continuous peanut cropping on dry matter weight of different peanut varieties, soil nutrient contents and enzyme activities. Soils, 2018, 50(3): 491-497]
[27] Tu C, Wei J, Guan F, et al. Biochar and bacteria inocu-lated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil. Environment International, 2020, 137: 105576
[28] 胡亚杰, 韦建玉, 张纪利, 等. 枯草芽孢杆菌对植烟土壤养分含量与酶活性的影响. 作物研究, 2019, 33(6): 561-566 [Hu Y-J, Wei J-Y, Zhang J-L, et al. Effect of Bacillus subtilis on soil nutrients content and enzymes activity. Crop Research, 2019, 33(6): 561-566]
[29] Batool TS, Ali S, Seleiman MF, et al. Plant growth promoting rhizobacteria alleviates drought stress in potato in response to suppressive oxidative stress and antioxidant enzymes activities. Scientific Reports, 2020, 10: 16975
[30] 周巍. 微生物肥在蔬菜生产上的应用. 现代农业, 2015(9): 37-38 [Zhou W. Application of microbial fertilizer in vegetable production. Modern Agriculture, 2015(9): 37-38]
[31] Gangireddygari VSR, Kanderi D, Golla R, et al. Biodegradation of quinalphos by a soil bacterium-Bacillus subtilis. Pakistan Journal of Biological Sciences, 2017, 20: 410-422

不同形态微生物菌剂对不结球白菜生长和品质的影响

Effects of different forms of microbial agents on the growth and quality of Brassica rapa L. ssp. chinensis Makino (non-heading Chinese cabbage).

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王剑武, 徐森, 季碧勇, 杜群. 地形和林分空间结构对浙江省天然阔叶混交林主要先锋树种胸径生长的影响 [J]. 应用生态学报, 2024, 35(2): 298-306.
[2]	杨颜萌, 张家兴, 李亚茹, 马靖然, 王铎, 靳占才, 谢路路, 邓娇娇, 叶吉, 于大炮, 王庆伟. 光质对黄芩生长与生理生化特征的影响 [J]. 应用生态学报, 2024, 35(2): 424-430.
[3]	甘菲菲, 招礼军, 霍灿灿, 王晟. 光照和水分对四季米仔兰幼苗生长的影响 [J]. 应用生态学报, 2024, 35(2): 439-446.
[4]	张雅婷, 叶旺敏, 熊德成, 吴晨, 黄锦学, 陈仕东, 杨智杰. 杉木幼树光合特性与生长的季节变化及其对土壤增温的响应 [J]. 应用生态学报, 2024, 35(1): 195-202.
[5]	吴欣阳, 邵静, 陈晓萍, 李锦隆, 胡丹丹, 钟全林, 程栋梁. 武夷山不同海拔阔叶树叶片养分含量及再吸收效率 [J]. 应用生态学报, 2023, 34(9): 2305-2313.
[6]	解萍萍, 张博奕, 董一博, 吕鹏程, 杜明超, 张先亮. 华北落叶松和白杄径向生长对干旱的生态弹性差异 [J]. 应用生态学报, 2023, 34(7): 1779-1786.
[7]	马凡强, 简尊吉, 郭泉水, 秦爱丽, 裴顺祥, 张光箭, 黄吉兰, 周李萍. 不同农林废弃物混合基质对崖柏扦插育苗的影响 [J]. 应用生态学报, 2023, 34(7): 1817-1824.
[8]	朱振闯, 孙仕军, 朱淼淼, 李咚祎, 王哲, 谌入瑄, 陈伟. 地下水埋深对东北平原地下水补给及大豆生长动态的影响 [J]. 应用生态学报, 2023, 34(7): 1871-1882.
[9]	张泽鹏, 金洪宇, 迟淼, 邵慧丽, 李雷. 西藏朗错兰格湖裸鲤的生长、死亡及资源利用状况 [J]. 应用生态学报, 2023, 34(7): 1988-1994.
[10]	闫明, 陈利顶, 孙然好. 城市生态品质的内涵及其核心指标体系构建 [J]. 应用生态学报, 2023, 34(6): 1459-1466.
[11]	娄运生, 于玉洁, 刘燕, 杨蕙琳, 周东雪. 施硅对夜间增温下南方水稻生长、产量和品质的影响 [J]. 应用生态学报, 2023, 34(4): 985-992.
[12]	李凤瑞, 赵文超, 张东楼, 董灵艳, 王汝明, 齐洪鑫, 张超, 张贵军, 杨秀凤, 史加亮. 黄河流域适宜机采的短季棉密度和行距配置 [J]. 应用生态学报, 2023, 34(4): 1002-1008.
[13]	刘阿康, 马瑞琦, 王德梅, 王艳杰, 杨玉双, 赵广才, 常旭虹. 冬前积温对北部冬麦区小麦苗期生长的影响 [J]. 应用生态学报, 2023, 34(3): 679-687.
[14]	张鸿志, 张文娥, 樊卫国, 欧阳章维, 蔡虎, 郝振坤, 王瑞璞, 潘学军. 核桃/刺梨复合种植对刺梨生长、果实产量、品质和土壤性质的影响 [J]. 应用生态学报, 2023, 34(3): 699-707.
[15]	阮文洁, 何云玲, 黄丽华. 滇中城市群植被物候时空变化及其对城市化的响应 [J]. 应用生态学报, 2023, 34(12): 3263-3270.