闽江口-平潭海域有机解磷菌多样性及群落特征

doi:10.13287/j.1001-9332.202105.038

应用生态学报 ›› 2021, Vol. 32 ›› Issue (5): 1863-1872.doi: 10.13287/j.1001-9332.202105.038

闽江口-平潭海域有机解磷菌多样性及群落特征

佘晨兴^1,2, 林洪¹, 苏玉萍^1,2*, 张勇^1,2, 林婉珍^1,2, 兰瑞芳^1,2

¹福建师范大学环境科学与工程学院, 福州 350007;
²福建省污染控制与资源循环利用重点实验室, 福州 350007

收稿日期:2020-08-09 接受日期:2021-02-14 出版日期:2021-05-15 发布日期:2021-11-15
通讯作者: *E-mail: ypsu@fjnu.edu.cn
作者简介:佘晨兴,男,1973年生,博士,副教授。主要从事微生物分子生态学、污染环境微生物修复技术、碳磷元素生物地球化学循环研究。E-mail:cxshe0089@sina.com
基金资助:
国家重点研发计划项目(2016YFE0202100)、国家自然科学基金项目(41573075)和福建省自然科学基金项目(2018J01742)资助

Diversity and community characteristics of organic phosphate-mineralizing bacteria in the sea area between Minjiang Estuary to Pingtan.

SHE Chen-xing^1,2, LIN Hong¹, SU Yu-ping^1,2*, ZHANG Yong^1,2, LIN Wan-zhen^1,2, LAN Rui-fang^1,2

¹College of Environmental Science and Engineering, Fujian Normal University, Fuzhou 350007, China;
²Fujian Key Laboratory of Pollution Control and Resource Reuse, Fuzhou 350007, China

Received:2020-08-09 Accepted:2021-02-14 Online:2021-05-15 Published:2021-11-15
Contact: *E-mail: ypsu@fjnu.edu.cn
Supported by:
National Key Research and Development Program of China (2016YFE0202100), the National Natural Science Foundation of China (41573075) and the Natural Science Foundation of Fujian Province (2018J01742).

摘要/Abstract

摘要： 有机磷的微生物矿化是海洋磷循环的重要环节,开展有机解磷菌研究有助于揭示富营养化海域有机磷矿化的微生物驱动机制。以phoX为标志基因,采用Illumina高通量测序技术分析了闽江口-平潭海域2019年4月(春季)及7月(夏季)有机解磷菌的多样性及群落特征。结果表明: 表层海水样品中有机解磷菌的Shannon指数介于3.21～7.91,各站位多样性春季均大于夏季;沉积物样品中有机解磷菌的Shannon指数介于2.04～8.70,各站位多样性夏季大于春季。春季各站位表层海水有机解磷菌的Shannon指数均高于沉积物,而夏季则相反。表层海水中检测到9个门类的有机解磷菌,主要为变形菌门和蓝细菌门;沉积物中则检测到12个门类,主要为变形菌门和拟杆菌门。在属水平上,有机解磷菌的群落组成呈现时空变异特征。表层海水样品中,春季以雷辛格氏菌属、褐杆菌属、深海球菌属和假单胞菌属等属为主,夏季则以聚球藻属、Halioglobus属、玫瑰变色菌属、褐杆菌属、亚硫杆菌属和生丝单胞菌属等属为主。在沉积物样品中,春季主要菌属包括雷辛格氏菌属、褐杆菌属、弧菌属和亚硫杆菌属;夏季主要菌属包括固氮螺丝菌属、氨基杆菌属、Sulfurifustis属、伯克氏菌属和Thiohalobacter属。此外,在表层海水和沉积物样品中均检测到大量未分类的有机解磷菌。冗余分析显示,溶解氧、水温、pH、可溶性无机氮、亚硝态氮、硝态氮等对闽江口-平潭海域表层海水有机解磷菌群落分布影响较大。表层海水和沉积物中存在的丰富有机解磷菌可能在该海域的磷循环中发挥重要作用。

关键词: 沉积物, 海水, 有机解磷菌, 高通量测序, phoX

Abstract: Microbial mineralization of organic phosphorus is an important component of marine phosphorus cycle. The research on organic phosphate-mineralizing bacteria (OPB) is helpful to reveal microbial driving mechanism of organic phosphorus mineralization in eutrophic sea area. The diversity and community characteristics of OPB were examined by Illumina high-throughput sequencing using the primer sets phoX in the sea area between Minjiang Estuary to Pingtan in April (spring) and July (summer) 2019. The results showed that the Shannon index of OPB in the surface seawater samples ranged from 3.21 to 7.91, and that the diversity at each station was greater in spring than that in summer. Shannon index of OPB in the sediment samples ranged from 2.04 to 8.70, which was greater in summer than that in spring. Shannon index of OPB in surface seawater of each station was higher than that of sediment in spring, while it was in adverse in summer. Nine phyla of OPB were detected in surface seawater, with Proteobacteria and Cyanobacteria being the most abundant. Tweleve phyla of OPB were detected in the sediments, with Proteobacteria and Bacteroidetes being the most dominant. OPB community composition at the genus level showed obvious spatio-temporal variation. Leisingera, Phaeobacter, Thalassococcus, and Pseudomonas were the major genera in the seawater in spring, while Synechococcus, Halioglobus, Roseovarius, Phaeo-bacter, Sulfitobacter, and Hyphomonas were the major genera detected in summer. Leisingera, Phaeobacter, Vibrio, and Sulfitobacter were major genera in the sediment in spring, while Azospirillum, Aminobacter, Sulfurifustis, Burkholderia, and Thiohalobacter were the major genera in summer. A large number of unclassified OPB were detected in both surface seawater and sediment. The redundancy analysis results showed that dissolved oxygen, water temperature, pH, dissolved inorganic nitrogen, NO₂^--N, and NO₃^--N had great influences on community distribution of OPB in the surface seawater. The abundant OPB in the surface seawater and sediment might play an important role in phosphorus cycle in this sea area.

Key words: sediment, surface seawater, organic phosphate-mineralizing bacteria, high-throughput sequencing, phoX

佘晨兴, 林洪, 苏玉萍, 张勇, 林婉珍, 兰瑞芳. 闽江口-平潭海域有机解磷菌多样性及群落特征[J]. 应用生态学报, 2021, 32(5): 1863-1872.

SHE Chen-xing, LIN Hong, SU Yu-ping, ZHANG Yong, LIN Wan-zhen, LAN Rui-fang. Diversity and community characteristics of organic phosphate-mineralizing bacteria in the sea area between Minjiang Estuary to Pingtan.[J]. Chinese Journal of Applied Ecology, 2021, 32(5): 1863-1872.

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闽江口-平潭海域有机解磷菌多样性及群落特征

Diversity and community characteristics of organic phosphate-mineralizing bacteria in the sea area between Minjiang Estuary to Pingtan.

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