植物-固定化菌剂联合修复多环芳烃污染土壤

doi:10.13287/j.1001-9332.202108.031

应用生态学报 ›› 2021, Vol. 32 ›› Issue (8): 2982-2988.doi: 10.13287/j.1001-9332.202108.031

植物-固定化菌剂联合修复多环芳烃污染土壤

李娜^1,2, 刘睿^1*, 台培东¹, 周星星¹, 肖瑶¹, 李玲美^1,2, 侯力群^1,2, 郑廷雨¹

¹中国科学院沈阳应用生态研究所中国科学院污染生态与环境工程重点实验室, 沈阳 110016;
²中国科学院大学, 北京 100049

收稿日期:2020-07-29 接受日期:2021-04-17 出版日期:2021-08-15 发布日期:2022-02-15
通讯作者: *E-mail: liurui@iae.ac.cn
作者简介:李娜, 女, 1991年生, 硕士研究生。主要从事污染土壤修复研究。E-mail: lina166@mails.ucas.ac.cn
基金资助:
沈阳市中青年科技创新人才支持计划项目(RC180179)资助

Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil

LI Na^1,2, LIU Rui^1*, TAI Pei-dong¹, ZHOU Xing-xing¹, XIAO Yao¹, LI Ling-mei^1,2, HOU Li-qun^1,2, ZHENG Ting-yu¹

¹Key Laboratory of Pollution Eco-logy and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
²University of Chinese Academy of Sciences, Beijing 100049, China

Received:2020-07-29 Accepted:2021-04-17 Online:2021-08-15 Published:2022-02-15
Contact: *E-mail: liurui@iae.ac.cn
Supported by:
Shenyang City Youth Science and Technology Innovation Talent Support Program (RC180179).

摘要/Abstract

摘要： 以火凤凰根际土壤中发现的3种优势菌[分枝杆菌(Ⅰ)、产黄纤维单胞菌(Ⅱ)、少动鞘氨醇单胞菌(Ⅲ)]构建的多菌剂体系为供试菌剂,针对大港油田原油污染土壤,将固定化供试菌剂接种于修复植物火凤凰根际,探讨供试菌剂强化火凤凰修复多环芳烃(PAHs)污染土壤的效果。结果表明: 处理ⅠⅢ(有效活菌数为10⁹ cfu·mL^-1)和ⅠⅡⅢ(有效活菌数为10⁷ cfu·mL^-1)对PAHs的降解有促进作用,PAHs降解率分别为32.2%和41.4%,均显著高于相应对照处理。此外,处理ⅠⅡⅢ对火凤凰的地下生物量有明显促进作用,比对照处理增加了31.2%。表明由3种优势菌构建的多菌剂ⅠⅡⅢ可以作为火凤凰修复PAHs污染土壤的强化手段,为微生物强化植物修复技术提供了新的修复思路及方法。

关键词: 固定化多菌剂, 火凤凰, 植物-微生物联合修复, 多环芳烃降解率, 石油污染土壤

Abstract: In this study, three dominant bacteria Cellulomonas flavigena (Ⅰ), Cellulomonas flavigena (Ⅱ), Sphingomonas paucimobilis (Ⅲ) from Fire Phoenix rhizosphere soil were used to develop a multi-microbial agent system. For oil-contaminated soil in the Dagang oilfield, the immobilized test bacteria were inoculated into the Fire Phoenix rhizosphere soil to examine the effects of bacterial agents on polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. The results showed that PAHs degradation was promoted under the ⅠⅢ (with an effective number of viable bacteria of 10⁹ cfu·mL^-1) and ⅠⅡⅢ (with an effective number of viable bacteria of 10⁷ cfu·mL^-1) treatments. The PAHs degradation rates were 32.2% and 41.4%, respectively, being significantly higher than that in the control treatments. The ⅠⅡⅢ treatment significantly promoted the belowground biomass of Fire Phoenix, which was 31.2% higher than that of the control treatment. Our results suggested that the multi-microbial agent constructed by the three dominant bacteria ⅠⅡⅢ could be used as a strengthening method for the remediation of PAHs-contaminated soil by Fire Phoenix, which provided a novel method for microbial enhanced phytoremediation technology.

Key words: immobilized multi-microbial agent, Fire Phoenix, plant-microorganism combined remediation, degradation rate of polycyclic aromatic hydrocarbon (PAHs), oil-contaminated soil

李娜, 刘睿, 台培东, 周星星, 肖瑶, 李玲美, 侯力群, 郑廷雨. 植物-固定化菌剂联合修复多环芳烃污染土壤[J]. 应用生态学报, 2021, 32(8): 2982-2988.

LI Na, LIU Rui, TAI Pei-dong, ZHOU Xing-xing, XIAO Yao, LI Ling-mei, HOU Li-qun, ZHENG Ting-yu. Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil[J]. Chinese Journal of Applied Ecology, 2021, 32(8): 2982-2988.

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植物-固定化菌剂联合修复多环芳烃污染土壤

Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil

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