缘管浒苔和浒苔对海水盐度胁迫的生理响应

应用生态学报 ›› 2012, Vol. 23 ›› Issue (07): 1913-1920.

缘管浒苔和浒苔对海水盐度胁迫的生理响应

高兵兵¹,郑春芳²,徐军田³,郑青松^1**,刘兆普¹,翟瑞婷¹,蒋和平¹

(¹南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室，南京 210095； ²浙江省近岸水域生物资源开发与保护重点实验室，浙江温州 325005； ³淮海工学院海洋学院, 江苏连云港 222005)

出版日期:2012-07-18 发布日期:2012-07-18

Physiological responses of Enteromorpha linza and Enteromorpha prolifera to seawater salinity stress.

GAO Bing-bing¹, ZHENG Chun-fang², XU Jun-tian³, ZHENG Qing-song¹, LIU Zhao-pu¹, ZHAI Rui-ting¹, JIANG He-ping¹

(¹Jiangsu Province Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; ²Zhejiang Province Key Laboratory of Exploitation and Preservation of Coastal Bioresource, Wenzhou 325005, Zhejiang, China; ³College of Oceanography, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China)

Online:2012-07-18 Published:2012-07-18

摘要/Abstract

摘要： 为探讨大型海藻对盐度的生理响应及其适应机制，以缘管浒苔和浒苔为试验材料，研究了不同盐度的稀释或浓缩海水处理10 d对浒苔属植物鲜质量（FM）、相对生长速率（RGR）、相对电导率（REC）、叶绿素含量（Chl）、类胡萝卜素含量（Car）、色素比值（Chl a/Chl b、Chl/Car）、叶绿素荧光参数和渗透调节能力（OAA）的影响.结果表明：与对照相比，10%～200%海水处理均明显促进两浒苔属品种的FM和RGR，缘管浒苔和浒苔分别在100%和50%海水处理下的FM和RGR达到最大值；300%海水处理显著抑制两浒苔生长，缘管浒苔受抑制程度较大；缘管浒苔的生物量仅在50%、100%海水处理下呈现正增长，浒苔生物量在10%、50%、100%、200%海水处理下均呈现正增长.10%海水处理下，两浒苔的Chl、Car、Chl a/Chl b显著上升，且随海水盐度的增加，呈现先增后降，缘管浒苔和浒苔的Chl、Car、Chl a/Chl b分别在100%、50%海水处理下达到最大值.随盐度的增加，叶绿素荧光参数PSⅡ最大光能转化效率（F_v/F_m）、PSⅡ实际光能转化效率（Yield）、最大相对电子传递速率（rETR_max）、光能利用效率（α）和半饱和光强（I_k）都显示与Chl相同的变化趋势.10%～300%海水处理下，浒苔属均表现出一定的OAA，缘管浒苔在100%海水处理下，OAA达到最大值，浒苔在50%海水处理下，OAA达到最大值.两浒苔的生长指标除与Chl/Car无明显的相关性，与REC呈极显著负相关，与Chl、Car、Chl a/Chl b、F_v/F_m、Yield、rETR_max、α、I_k、OAA呈极显著正相关.100%和50%海水处理分别对缘管浒苔和浒苔的生长最适宜，浒苔生长适应盐度的范围比缘管浒苔宽.REC、Chl、Car、Chl a/Chl b、F_v/F_m、Yield、rETR_max、α、I_k和OAA均可以作为浒苔属植物生长盐适应性的评价指标.

Abstract: To investigate the physiological responses and adaptation mechanisms of Enteromorpha to seawater salinity stress, a laboratory experiment with Enteromorpha linza and E. prolifera was conducted to study their fresh mass (FM), relative growth rate (RGR), relative electrical conductivity (REC), chlorophyll (Chl) and carotenoid (Car) contents, Chl a/Chl b, Chl/Car, chlorophyll fluorescence parameters, and osmotic adjustment ability (OAA) under the stress of different salinity levels of diluted and concentrated seawater for 10 days. Compared with the control, 10%-200% salinity seawater increased the FM and RGR of the two Enteromorpha species obviously, 100% and 50% salinity seawater made the FM and RGR of E. linza and E. prolifera peaked, respectively, while 300% salinity seawater decreased the FM and RGR of E. linza and E. prolifera significantly, with the decrement being larger for E. linza. The biomass of E. linza and E. prolifer only had an increase in 50% and 100% sanity seawater and in 10%, 50%, 100%, and 200% salinity seawater, respectively. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera had a significant increase in 10% salinity seawater, but decreased after an initial increase with the increasing salinity level of seawater. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera peaked in 100% and 50% salinity seawater, respectively. With increasing salinity of seawater, the light use efficiency (α), maximal photochemical efficiency of PSⅡ(F_v/F_m), actual photochemical efficiency of PSⅡ in the light (Yield), maximal relative electron transport rate (rETR_max), and halfsaturation light intensity (I_k) of E. linza and E. prolifera all showed the same variation trend as Chl. 10%-300% salinity seawater enabled E. linza and E. prolifera to express certain osmotic adjustment ability (OAA), and the OAA of E. linza and E. prolifer peaked in 100% and 50% salinity seawater, respectively. The growth of Enteromorpha had no correlation with Chl/Car, but was significantly negatively correlated with REC and positively correlated with Chl, Car, Chl a/Chl b, F_v/F_m, Yield, rETR_max, α, I_k, and OAA. To sum up, 100% salinity was the optimal salt concentration for the growth of E. linza, and 50% salinity was optimal for E. prolifera. E. prolifera could adapt to a wider range of salinity than E. linza. The parameters REC, Chl, Car, Chl a/Chl b, F_v/F_m, Yield, rETR_max, α, I_k, and OAA could be used to evaluate the salt adaptation of Enteromorpha.

高兵兵1,郑春芳2,徐军田3,郑青松1**,刘兆普1,翟瑞婷1,蒋和平1. 缘管浒苔和浒苔对海水盐度胁迫的生理响应[J]. 应用生态学报, 2012, 23(07): 1913-1920.

GAO Bing-bing1, ZHENG Chun-fang2, XU Jun-tian3, ZHENG Qing-song1, LIU Zhao-pu1, ZHAI Rui-ting1, JIANG He-ping1. Physiological responses of Enteromorpha linza and Enteromorpha prolifera to seawater salinity stress.[J]. Chinese Journal of Applied Ecology, 2012, 23(07): 1913-1920.