油菜素内酯对低氧胁迫下黄瓜幼苗叶片线粒体、叶绿体超微结构及光合的影响

应用生态学报 ›› 2012, Vol. 23 ›› Issue (08): 2205-2211.

油菜素内酯对低氧胁迫下黄瓜幼苗叶片线粒体、叶绿体超微结构及光合的影响

陆晓民^1,2,孙锦¹,郭世荣^1**,王军伟¹

(¹南京农业大学园艺学院农业部南方蔬菜遗传改良重点开放实验室, 南京 210095; ²安徽科技学院, 安徽凤阳 233100)

出版日期:2012-08-18 发布日期:2012-08-18

Effects of brassinolide on the leaf mitochondria and chloroplast ultrastructure and photosynthesis of cucumber seedlings under hypoxia stress.

LU Xiao-min^1,2, SUN Jin¹, GUO Shi-rong¹, WANG Jun-wei¹

(¹Ministry of Agriculture Key Laboratory of Southern Vegetable Crop Genetic Improvement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; ²Anhui Science and Technology University, Fengyang 233100, Anhui, China)

Online:2012-08-18 Published:2012-08-18

摘要/Abstract

摘要： 在水培条件下，研究24-表油菜素内酯(EBR)对低氧胁迫下黄瓜幼苗叶片叶绿体和线粒体超微结构及光合的影响.结果表明：与正常通气条件相比，低氧胁迫下表观量子效率(AQY)和羧化效率(CE)显著降低，而光补偿点(LCP)、暗呼吸速率(R_d)和CO₂补偿点(CCP)显著升高；低氧胁迫并添加油菜素内酯处理下，CE与低氧胁迫处理相比显著提高29.4%，而LCP和R_d分别显著下降15.0%和14.4%.光响应P_nPPFD曲线和CO₂响应P_n-C_i曲线表明，低氧胁迫下净光合速率(P_n)增幅降低，而添加油菜素内酯有利于P_n增幅的提高.低氧胁迫下叶绿体和线粒体结构受到伤害，而油菜素内酯可以缓解低氧胁迫对黄瓜幼苗叶绿体和线粒体超微结构的不良影响，使叶片维持较好的光合性能.

Abstract: A hydroponic experiment was conducted to investigate the effects of 24-epibrassinolide (EBR) on the leaf mitochondria and chloroplast ultrastructure and photosynthesis of cucumber seedlings under hypoxia stress. Under the stress, the apparent quantum yield (AQY) and carboxylation efficiency (CE) decreased significantly, while the light compensation point (LCP), dark breathing rate (R_d), and CO₂ compensation point (CCP) had a significant increase. The application of EBR increased the CE significantly by 29.4%, and decreased the LCP and R_d significantly by 15.0% and 14.4%, respectively. The light response curve (P_nPPFD) and CO₂ response curve (P_n-C_i) indicated that under hypoxia stress, the increment of net photosynthetic rate (P_n) decreased, while EBR addition enhanced the increment of the P_n. Hypoxia stress damaged the mitochondria and chloroplast ultrastructure, while EBR could alleviate the damages in chloroplast and mitochondria under hypoxia stress, and keep the leaves in a higher photosynthetic performance.

陆晓民1,2,孙锦1,郭世荣1**,王军伟1. 油菜素内酯对低氧胁迫下黄瓜幼苗叶片线粒体、叶绿体超微结构及光合的影响[J]. 应用生态学报, 2012, 23(08): 2205-2211.

LU Xiao-min1,2, SUN Jin1, GUO Shi-rong1, WANG Jun-wei1. Effects of brassinolide on the leaf mitochondria and chloroplast ultrastructure and photosynthesis of cucumber seedlings under hypoxia stress.[J]. Chinese Journal of Applied Ecology, 2012, 23(08): 2205-2211.