Abstract: By using modified Levins and Petraitis formulae, this paper determined the niche breadth and niche overlap of the phytoplankton’s dominant species in Dianshan Lake of East China, and analyzed the relationships between the niche breadth and niche overlap and the density and dominance of the dominant species. The niche breadth and niche overlap of the dominant species differed in different periods, and different dominant species had different adaptive capacity to the environmental factors. Based on their niche breadth in different seasons, the dominant species in the Lake could be classified into four groups, among which, Chroomonas acuta and Chlorella vulgaris had broader niche, more quantity, wider distribution, and better use of environmental resources. During cyanobacterial blooms, the niche overlap among Cyanophyta species was comparatively higher, Microcystis aeruginosa had broader niche breadth, but other species showed lower niche breadth. Correlation analysis showed that the dominance of the dominant species in different seasons had significant correlation with their niche breadth, and the dominant species density had significant correlation with their niche overlap.