长江口低氧区沉积物表层纤毛虫多样性及其时空变化

应用生态学报

长江口低氧区沉积物表层纤毛虫多样性及其时空变化

赵峰^1,2,徐奎栋^1**,孟昭翠¹

(¹中国科学院海洋研究所海洋生物分类与系统演化实验室, 山东青岛 266071; ²中国科学院大学, 北京 100049)

出版日期:2012-12-18 发布日期:2012-12-18

Ciliate diversity and spatiotemporal variation in surface sediments of Yangtze River estuary hypoxic zone.

ZHAO Feng^1,2, XU Kui-dong¹, MENG Zhao-cui¹

(¹Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, China; ²University of the Chinese Academy of Sciences, Beijing 100049, China)

Online:2012-12-18 Published:2012-12-18

摘要/Abstract

摘要： 利用PCR-DGGE(变性梯度凝胶电泳)指纹图谱和测序技术,以及Ludox-QPS(密度梯度离心定量蛋白银染色)方法,研究了2011年4月和8月长江口低氧区3个站位表层沉积物中纤毛虫多样性及季节变化.结果表明: 不同站位之间纤毛虫分子多样性存在显著差异(ANOSIM分析:R=0.896, P=0.0001),但季节变化不显著(R=0.043, P=0.207).序列数最多的类群为旋唇纲中的寡毛类和舞毛类纤毛虫.Ludox-QPS法研究的纤毛虫活动虫体的种类数及丰度可维持在较高的水平,且在夏季增高2~5倍.Ludox-QPS法与DGGE技术检测到的不同站位间纤毛虫多样性变化趋势基本一致.但Ludox-QPS法检测到纤毛虫活动虫种类数及丰度随季节更替变化显著,该法检获的纤毛虫种类数高于DGGE条带数.长江口低氧区的纤毛虫丰度及多样性较高,可为潜在的水母水螅体提供食物支撑.

Abstract: By using denaturing gradient gel electrophoresis (DGGE) and sequencing as well as Ludox-QPS method, an investigation was made on the ciliate diversity and its spatiotemporal variation in the surface sediments at three sites of Yangtze River estuary hypoxic zone in April and August 2011. The ANOSIM analysis indicated that the ciliate diversity had significant difference among the sites (R=0.896, P=0.0001), but less difference among seasons (R=0.043, P=0.207). The sequencing of 18S rDNA DGGE bands revealed that the most predominant groups were planktonic Choreotrichia and Oligotrichia. The detection by Ludox-QPS method showed that the species number and abundance of active ciliates were maintained at a higher level, and increased by 2-5 times in summer, as compared with those in spring. Both the Ludox-QPS method and the DGGE technique detected that the ciliate diversity at the three sites had the similar variation trend, and the Ludox-QPS method detected that there was a significant variation in the ciliate species number and abundance between different seasons. The species number detected by Ludox-QPS method was higher than that detected by DGGE bands. Our study indicated that the ciliates in Yangtze River estuary hypoxic zone had higher diversity and abundance, with the potential to supply food for the polyps of jellyfish.

赵峰1,2,徐奎栋1**,孟昭翠1. 长江口低氧区沉积物表层纤毛虫多样性及其时空变化[J]. 应用生态学报.

ZHAO Feng1,2, XU Kui-dong1, MENG Zhao-cui1. Ciliate diversity and spatiotemporal variation in surface sediments of Yangtze River estuary hypoxic zone.[J]. Chinese Journal of Applied Ecology.