可可西里低温适生拮抗芽孢杆菌的筛选鉴定及脂肽化合物分析

应用生态学报

可可西里低温适生拮抗芽孢杆菌的筛选鉴定及脂肽化合物分析

谢永丽^1,2,高学文^2**

(¹青海大学农牧学院高原草地资源与生态省部共建重点实验室，西宁 810016； ²南京农业大学植物保护学院农作物生物灾害综合治理农业部重点实验室，南京 210095)

出版日期:2013-01-18 发布日期:2013-01-18

Screening and identification of low temperature-adapted antagonistic Bacillus isolated from Kekexili region of West China and the analysis of the isolates lipopeptide compounds.

XIE Yong-li^1,2, GAO Xue-wen²

(¹Qinghai Province and Ministry of Education Coconstructing Key Laboratory of Plateau Grassland Resource and Ecology, Qinghai University, Xining 810016, China; ²Ministry of Agriculture Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China).

Online:2013-01-18 Published:2013-01-18

摘要/Abstract

摘要：

极端环境特殊微生物资源研究和开发具有广阔的应用前景和研究意义.对分离筛选自青海可可西里境内植被根围的8株在4和10 ℃条件下生长良好的低温适生芽孢杆菌进行鉴定分析.结果表明: 通过生理生化特征分析、rep-PCR指纹图谱分析、16S rDNA及gyrB基因序列分析鉴定，8株供试菌株分别为莫哈韦芽孢杆菌(Bacillus mojavensis)3株,解淀粉芽孢杆菌(B. amyloliquefaciens)1株和简单芽孢杆菌(B. simplex)4株.采用平板对峙试验从中筛选到4株对油菜菌核病原真菌(Sclerotinia sclerotiorum)及水稻白叶枯病原细菌(Xanthomonas oryzae pv. oryzae)均具有显著拮抗活性的生防菌株；采用MALDI-TOF-MS质谱分析生防菌株的拮抗活性物质，结果显示菌株KKD-1 (B. mojavensis)产生脂肽类化合物泛革素和表面活性素，菌株KKD-2(B. amyloliquefaciens)产生脂肽类化合物伊枯草菌素A、泛革素和表面活性素，推断生防菌株的拮抗活性可能与脂肽化合物的合成及分泌有关.该研究为低温适生性芽孢杆菌生物肥料和生物农药的研发提供了菌株资源.

Abstract: The research and exploitation of special microbial resources in extreme environment is of scientific significance and has broad applied prospect. In this paper, eight Bacillus strains isolated from the vegetation rhizospheres in Kekexili extreme region of Qinghai Province and presented good growth status at low temperature 4 and 10 ℃ were identified. Through physiological and biochemical analysis,
rep-PCR fingerprinting, and 16S rDNA and gyrB partial sequence analyses, the eight strains were identified as Bacillus mojavensis (3 isolates), Bacillus amyloliquefaciens (1 isolate), and Bacillus simplex (4 isolates). The agar plate antagonistic test showed that four of the isolates presented distinct antagonistic activity to Sclerotinia sclerotiorum and Xanthomonas oryzae pv. oryzae. The MALDI-TOF-MS analysis showed that the strain KKD-1 (B. mojavensis) produced fengycin and surfactin, whereas the strain KKD-2 (B. amyloliquefaciens) produced iturin A, surfactin and fengycin, suggesting that the biocontrol efficacy of the Bacillus strains could be related to the synthesis and excretion of the antifungal lipopeptide compounds. This study provided the bacterial resources for the research and exploitation of low temperatureadapted Bacillus biofertilizers and bio-pesticides.

谢永丽1,2,高学文2**. 可可西里低温适生拮抗芽孢杆菌的筛选鉴定及脂肽化合物分析[J]. 应用生态学报.

XIE Yong-li1,2, GAO Xue-wen2. Screening and identification of low temperature-adapted antagonistic Bacillus isolated from Kekexili region of West China and the analysis of the isolates lipopeptide compounds.[J]. Chinese Journal of Applied Ecology.