Abstract: Laboratory incubation test was conducted to study the effects of temperature on the CH₄ emission from the leaves of subtropical common tree species Castanopsis carlesii, Schima superb, Cinnamomum chekiangense, Castsanopsis fabri, Cunninghamia lanceolata, and Citrus reticulata. Among the six tree species, only S. superb, C. reticulate, and C. fabri emitted CH₄ at 10 ℃. At above 20 ℃, all the six species emitted CH₄, and the average CH₄emission rate at above 30 ℃ (1.010 ng CH₄·g^-1DM·h^-1) was 2.96 times higher than that at 10-30 ℃ (0.255 ng CH₄·g^-1 DM·h^-1). Moreover, increasing temperature had much more effects on the CH₄ emission rate of C. reticulata and C. lanceolata than on that of the other four tree species. Incubation time affected the CH₄ emission rate of all test tree species significantly, suggesting that the effects of temperature stress on the CH₄ emission could be controlled by plant activity. Dry leaves could not emit CH₄ no matter the temperature was very high or low. It was suggested that high temperature stress had important effects on the CH₄ emission from subtropical tree leaves, and global warming could increase the CH₄ emission from plants.