南亚热带3种人工林土壤微生物生物量和微生物群落结构特征

应用生态学报

南亚热带3种人工林土壤微生物生物量和微生物群落结构特征

王卫霞¹,史作民^1**,罗达¹,刘世荣¹,卢立华²

(¹中国林业科学研究院森林生态环境与保护研究所, 国家林业局森林生态环境重点实验室, 北京 100091;
²中国林业科学研究院热带林业实验中心, 广西凭祥 532600)

出版日期:2013-07-18 发布日期:2013-07-18

Characteristics of soil microbial biomass and community composition in three types of plantations in southern subtropical area of China.

WANG Wei-xia¹, SHI Zuo-min¹, LUO Da¹, LIU Shi-rong¹, LU Li-hua²

(¹State Forestry Administration Key Laboratory on Forest Ecology and Environmental Sciences, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; ²Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, Guangxi, China)

Online:2013-07-18 Published:2013-07-18

摘要/Abstract

摘要： 以我国南亚热带格木、红椎和马尾松人工林为对象,采用氯仿熏蒸浸提法和磷脂脂肪酸法(PLFA)分析了林地土壤微生物生物量和微生物群落结构组成.结果表明: 林分和季节因素均显著影响土壤微生物生物量、总PLFAs量、细菌PLFAs量和真菌PLFAs量,且干季林分下的土壤微生物生物量、总PLFAs量、单个PLFA量均大于雨季.红椎人工林土壤微生物生物量碳(MBC)和总PLFAs量最高,而格木人工林土壤微生物生物量氮(MBN)最高.土壤pH值对土壤丛枝菌根真菌(16:1ω5c)的影响达到极显著正相关水平.土壤总PLFAs量、革兰氏阳性菌(G⁺)以及腐生真菌(18:2ω6,9c)、革兰氏阳性菌/革兰氏阴性菌(G⁺/G^-)与土壤有机碳、全氮和全磷显著相关,表明土壤有机碳、全氮、全磷含量是影响该地区土壤微生物数量和种类的重要因素.外生菌根真菌(18:1ω9c)和丛枝菌根真菌与土壤碳氮比值呈极显著相关.

Abstract: By using fumigationextraction method and phospholipid fatty acids (PLFAs) analysis, this paper studied the characteristics of soil microbial biomass and community composition in the Erythrophleum fordii, Castanopsis hystrix, and Pinus massoniana plantations in south subtropical China. The soil microbial biomass, total PLFAs, bacterial PLFAs, and fungal PLFAs in the plantations were significantly affected by the plantation type and season, and the soil microbial biomass, total PLFAs, and individual PLFA signatures were higher in dry season than in rainy season. The C. hystrix plantation had the highest soil microbial biomass carbon and total PLFAs, while the E. fordii plantation had the highest soil microbial biomass nitrogen. There was a significant positive correlation between the soil pH and arbuscular mycorrhizal fungal (AMF) PLFA (16:1ω5c). The soil total PLFAs, grampositive bacterial PLFAs, saprophytic fungal PLFA (18:2ω6,9c), and the ratio of gram-positive to gram-negative bacterial PLFAs were significantly positively correlated with soil organic carbon, total nitrogen, and total phosphorus, suggesting that the soil organic carbon, total nitrogen, and total phosphorus contents were the most important nutrient factors affecting the numbers and types of the soil microorganisms. In addition, the ectomycorrhizae fungal PLFA
(18:1ω9c) and AMF PLFA were significantly correlated with the soil C/N ratio.

王卫霞1,史作民1**,罗达1,刘世荣1,卢立华2. 南亚热带3种人工林土壤微生物生物量和微生物群落结构特征[J]. 应用生态学报.

WANG Wei-xia1, SHI Zuo-min1, LUO Da1, LIU Shi-rong1, LU Li-hua2. Characteristics of soil microbial biomass and community composition in three types of plantations in southern subtropical area of China.[J]. Chinese Journal of Applied Ecology.