氨基糖单体碳氮同位素的分析及其应用

doi:10.13287/j.1001-9332.202005.021

应用生态学报 ›› 2020, Vol. 31 ›› Issue (5): 1753-1762.doi: 10.13287/j.1001-9332.202005.021

氨基糖单体碳氮同位素的分析及其应用

郭金强^1,2,3,4, 袁华茂^1,2,3,4*, 宋金明^1,2,3,4, 李学刚^1,2,3,4, 李宁^1,2,3,4, 段丽琴^1,2,3,4

¹中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071;
²青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237;
³中国科学院大学, 北京 100049;
⁴中国科学院海洋大科学研究中心, 山东青岛 266071

收稿日期:2019-10-30 出版日期:2020-05-15 发布日期:2020-05-15
通讯作者: * E-mail: yuanhuamao@qdio.ac.cn
作者简介:郭金强, 男, 1993年生, 博士研究生。主要从事海洋生物地球化学研究。E-mail: guojinqiang19@mails.ucas.ac.cn
基金资助:
山东省重大科技创新工程专项(2018SDKJ0504-1)和中国科学院战略先导性专项(XDA23050501)资助

Compound-specific carbon and nitrogen isotope analysis of amino sugars and their applications

GUO Jin-qiang^1,2,3,4, YUAN Hua-mao^1,2,3,4*, SONG Jin-ming^1,2,3,4, LI Xue-gang^1,2,3,4, LI Ning^1,2,3,4, DUAN Li-qin^1,2,3,4

¹Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, China;
²Laboratory for Marine Ecology and Environmental Sciences, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, Shandong, China;
³University of Chinese Academy of Sciences, Beijing 100049, China;
⁴Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, Shandong, China

Received:2019-10-30 Online:2020-05-15 Published:2020-05-15
Contact: * E-mail: yuanhuamao@qdio.ac.cn
Supported by:
This work was supported by the Key Science and Technology Innovation Program of Shandong Province (2018SDKJ0504-1), and the Strategic Priority Research Program of the Chinese Aca-demy of Sciences (XDA23050501).

摘要/Abstract

摘要： 氨基糖(AS)作为有机质中在分子水平识别的重要组分,研究其来源与转化能更好地认知微生物对有机质的调控作用。作为一种新兴技术,氨基糖单体同位素分析(CSIA-AS)为研究氨基糖各组分在自然环境中的变化特征提供了更详细的信息。本文系统总结了CSIA-AS技术的测定方法及其在氨基糖循环转化研究中的应用,气相色谱-同位素比值质谱法(GC-IRMS)和离子色谱-同位素比值质谱法(IC-IRMS)作为2种主要的氨基糖同位素测定方法,各有利弊,但进行相应的校正后均可实现可靠的测定结果。氨基糖各组分在土壤有机质中具有相对较低的周转时间,细菌来源的胞壁酸相对葡萄糖胺、半乳糖胺和甘露糖胺具有更高的矿化速率。氨基糖在环境中的来源和代谢转化受底物的影响,这与微生物群落对不同碳、氮源的特异性响应有关。CSIA-AS技术的推广需要进一步的方法优化并将其与微生物甄别等其他手段相结合,以此来更好地阐释有机质的来源、转化和归宿及其调控机制。

关键词: 氨基糖单体同位素分析, 有机质, 周转, 矿化, 代谢转化

Abstract: Amino sugars (AS) are one of the important biochemical components in the natural organic matter pool. Clarifying the sources and transformations of AS would facilitate our understan-ding of the microbial regulation of organic matter. As an emerging technology, compound-specific isotope analysis of amino sugars (CSIA-AS) provides more detailed dynamic information of indivi-dual AS in natural environment. Here, we systematically summarized the determination methods of CSIA-AS and gave an overview on innovative applications in the cycling of AS. CSIA-AS can be performed by gas chromatography-isotope ratio mass spectrometry (GC-IRMS) and ion chromatography-isotope ratio mass spectrometry (IC-IRMS). Each method has its own advantages and disadvantages, but reliable results can be achieved after calibration. The mean residence time of AS is relatively low in soil organic matter, and the bacterial-derived muramic acid possesses a higher minera-lization rate than glucosamine, galactosamine, and mannosamine. The source and metabolic transformation of AS are affected by the substrate, which is related to the specific response of microbial community to different carbon and nitrogen sources. The promotion of CSIA-AS technology requires further optimization of method and integration with other approaches such as microbial screening to decipher the source, transformation, fate and regulatory mechanisms of organic matter.

Key words: compound-specific isotope analysis of amino sugars, organic matter, turnover, mine-ralization, metabolic transformation

郭金强, 袁华茂, 宋金明, 李学刚, 李宁, 段丽琴. 氨基糖单体碳氮同位素的分析及其应用[J]. 应用生态学报, 2020, 31(5): 1753-1762.

GUO Jin-qiang, YUAN Hua-mao, SONG Jin-ming, LI Xue-gang, LI Ning, DUAN Li-qin. Compound-specific carbon and nitrogen isotope analysis of amino sugars and their applications[J]. Chinese Journal of Applied Ecology, 2020, 31(5): 1753-1762.

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氨基糖单体碳氮同位素的分析及其应用

Compound-specific carbon and nitrogen isotope analysis of amino sugars and their applications

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