中国东北地区远东鼩鼱遗传多样性与遗传结构

doi:10.13287/j.1001-9332.202002.038

应用生态学报 ›› 2020, Vol. 31 ›› Issue (2): 634-642.doi: 10.13287/j.1001-9332.202002.038

中国东北地区远东鼩鼱遗传多样性与遗传结构

刘铸^1*, 汪青青¹, 白薇¹, 李博琦¹, 田新民^1,2, 李殿伟^1,2, 张隽晟¹

¹牡丹江师范学院生命科学与技术学院, 黑龙江牡丹江 157012;
²东北林业大学野生动物资源学院, 哈尔滨 150040

收稿日期:2019-09-18 出版日期:2020-02-15 发布日期:2020-02-15
通讯作者: * E-mail: swxlz0@126.com
作者简介:刘铸, 男, 1979年生, 博士。主要从事野生动物保护与分子生物学研究。E-mail: swxlz0@126.com
基金资助:
本文由黑龙江省教育厅项目(1354ZD004,1354MSYYB005)和黑龙江省自然科学基金项目(C2017065)资助

Genetic diversity and genetic structure of Sorex isodon in Northeast China

LIU Zhu^1*, WANG Qing-qing¹, BAI Wei¹, LI Bo-qi¹, TIAN Xin-min^1,2, LI Dian-wei^1,2, ZHANG Jun-sheng¹

¹College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157012, Heilongjiang, China;
²College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China

Received:2019-09-18 Online:2020-02-15 Published:2020-02-15
Contact: * E-mail: swxlz0@126.com
Supported by:
This work was supported by the Project of Department of Education in Heilongjiang Province (1354ZD004, 1354MSYYB005) and the Natural Science Fund of Heilongjiang Province (C2017065).

摘要/Abstract

摘要： 对中国东北地区3个种群(大兴安岭、小兴安岭和长白山山脉)的远东鼩鼱(77个样本)Cyt b基因全序列进行分析,共获得64个单倍型。整体单倍型多态性为0.9920,核苷酸多态性为0.0105,表明该地区远东鼩鼱具有较高遗传多样性,且长白山山脉远东鼩鼱种群遗传多样性明显高于大兴安岭和小兴安岭种群。F-统计量、遗传相似系数和遗传距离分析结果均显示,种群间和采样地间的遗传距离与地理距离基本相符。方差分析显示,种群间的变异占总变异的33.4%,种群内的采样地间变异占总变异的10.2%,采样点内部变异占总变异的56.4%。种群历史分析显示,东北地区远东鼩鼱未经历过数量扩张。从GenBank下载了欧亚其他地区远东鼩鼱序列进行遗传结构研究。远东鼩鼱系统发生树分化为2大分支:一大支主要由大兴安岭和小兴安岭种群构成,两种群具有一定分化;另一大支又分为两个分支。中介网络图显示,远东鼩鼱具有3个谱系:一个谱系主要由大兴安岭和小兴安岭的单倍型样本构成,还包括长白山山脉的4个单倍型样本;另一谱系包括来自于中国东北地区3个种群的个别单倍型,还包括俄罗斯贝加尔湖单倍型和芬兰单倍型;最后一个谱系完全是由长白山山脉单倍型构成。遗传多样性、系统发生树和中介网络图结果均表明,长白山山脉为远东鼩鼱末次冰期避难所。

Abstract: A total of 64 haplotypes were obtained from the complete Cytochrome b gene (Cyt b) of 77 Sorex isodon collected from three populations (Daxing’anling, Xiaoxing’anling, and Changbai Mountains) in Northeast China. The haplotype diversity was 0.9920 and the nucleotide diversity was 0.0105, indicating high genetic diversity. The genetic diversity of Changbai Mountains population was significantly higher than that of Daxing’anling and Xiaoxing’anling populations. The F-statistics, the number of migrants per generation and the genetic distance results showed that the genetic distances among the populations and among the sampling sites were generally consistent with geographical distance. Analysis of molecular variance showed that the differentiation among populations, among sampling sites, and within sampling site accounted for 33.4%, 10.2% and 56.4% of total variation, respectively. The analysis of population history showed that S. isodon in Northeast China experienced no population expansion. The reported complete sequence of Cyt b gene of S. isodon (GenBank) of Europe and other parts of Asia was downloaded to examine the genetic structure of S. isodon. The phylogenetic tree was divided into two large branches. One branch consisted mainly of Daxing’anling and Xiaoxing’anling samples. The other branch was departed into two sub-branches. Median-joining network analysis showed that there were three lineages: one lineage mainly consisted of haplotypes from Daxing’anling and Xiaoxing’anling, and also four haplotypes of Changbai Mountains, while the other lineage included a few haplotypes of three populations in Northeast China, and those from Baikal Lake, Russia and Finland. The last lineage was entirely composed of haplotypes from Changbai Mountains. The results of genetic diversity, phylogenetic tree and median-joining network all suggested that the Changbai Mountains was the refuge for S. isodon during last glacial.

刘铸, 汪青青, 白薇, 李博琦, 田新民, 李殿伟, 张隽晟. 中国东北地区远东鼩鼱遗传多样性与遗传结构[J]. 应用生态学报, 2020, 31(2): 634-642.

LIU Zhu, WANG Qing-qing, BAI Wei, LI Bo-qi, TIAN Xin-min, LI Dian-wei, ZHANG Jun-sheng. Genetic diversity and genetic structure of Sorex isodon in Northeast China[J]. Chinese Journal of Applied Ecology, 2020, 31(2): 634-642.

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中国东北地区远东鼩鼱遗传多样性与遗传结构

Genetic diversity and genetic structure of Sorex isodon in Northeast China

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