共生菌促进斜生栅藻生长和油脂合成

doi:10.13287/j.1001-9332.202002.034

应用生态学报 ›› 2020, Vol. 31 ›› Issue (2): 625-633.doi: 10.13287/j.1001-9332.202002.034

共生菌促进斜生栅藻生长和油脂合成

段露露, 程蔚兰, 张靖洁, 马浩天, 季春丽, 崔红利, 王计平, 李润植^*

山西农业大学分子农业与生物能源研究所, 山西太谷 030801

收稿日期:2019-06-21 出版日期:2020-02-15 发布日期:2020-02-15
通讯作者: * E-mail: rli2001@126.com
作者简介:段露露, 女, 1992年生, 硕士研究生。主要从事植物生理生态研究。E-mail: 964619274@qq.com
基金资助:
本文由国家自然科学基金项目(31201266,31401430,31902344)、农业部“948”项目(2014-Z39)、山西省煤基重大科技专项(FT-2014-01)、山西省留学基金项目(2015-064)、山西省重点研发重点项目(201603D312005)和山西省重点研发一般项目(201803D31063)资助

Symbiotic bacteria facilitate algal growth and oil biosynthesis in Scenedesmus obliquus

DUAN Lu-lu, CHEN Wei-lan, ZHANG Jing-jie, MA Hao-tian, JI Chun-li, CUI Hong-li, WANG Ji-ping, LI Run-zhi^*

Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, Shanxi, China

Received:2019-06-21 Online:2020-02-15 Published:2020-02-15
Contact: * E-mail: rli2001@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31201266, 31401430, 31902344), the Ministry of Agriculture “948” Project (2014-Z39), the Shanxi Province Key Project of Coal-based Science and Technology (FT-2014-01), the Shanxi Scholarship Fund (2015-064), the Key Project of the Key Research and Development Program of Shanxi Province (201603D312005), and the Key Research and Development (R&D) General Progam of Shanxi Province (201803D31063).

摘要/Abstract

摘要： 从斜生栅藻藻际分离共生菌群并进行16S rDNA序列鉴定,构建藻菌共培养体系,并检测斜生栅藻生长、生理生化及产油特性。共分离出7个菌群/株,包括微球菌(菌株1-1、1-2和1-3)、假单胞菌(菌株2-1和2-2)、微小杆菌(菌株-3)和葡萄球菌(菌株-4)。微球菌(菌株1-2)和假单胞菌(菌株2-1)为优势促生菌株,能显著促进微藻生长及色素和油脂积累。斜生栅藻与微球菌(菌株1-2)1∶10共培养体系培养8 d后,栅藻生物量高达4.27 g·L^-1,比对照增加了46.0%;叶绿素a、叶绿素b和类胡萝卜素分别比对照提高12.1%、16.7%和25.0%;含油量为25.7%,比对照增加了14.0%,单不饱和油酸含量(16.4%)也显著高于对照。另一个优异共培养体系是斜生栅藻与假单胞菌(菌株2-1)1∶5共培养体系,在培养8 d后,微藻生物量、叶绿素a、叶绿素b和类胡萝卜素分别比对照提高47.9%、16.0%、17.5%和19.9%;总油脂(27.1%)和单不饱和油酸含量(18.2%)分别比对照增加了20.4%和64.0%。表明藻际共生菌假单胞菌和微球菌可分别与斜生栅藻互作,能够显著促进斜生栅藻生物量和优质油脂的富集,可应用于栅藻商业生产。

Abstract: We isolated bacteria from the phycosphere of Scenedesmus obliques and sequenced 16S rDNAs to establish algae-bacterial co-culture systems. Further, we examined effects of the bacteria on algal growth, and parameters associated with physio-biochemical and oil-producing characteristics of S. obliquus. Seven bacterial strains were isolated, including Micrococcus (strains 1-1, 1-2 and 1-3), Pseudomonas sp. (strains 2-1 and 2-2), Exiguobacterium (strain-3) and Staphylococcus sp. (strain-4). Among them, two bacteria (strain 1-2 and strain 2-1) were characterized as the dominant growth-promoting bacterial strains, which significantly increased algal growth, pigment production, and oil enrichment. After eight days cultivation under microalgal-bacterial (strain 1-2) symbiotic systemat at an initial ratio of 1:10, biomass of S. obliquus was 4.27 g·L^-1, about 46.0% higher than that of the control. The contents of chlorophyll a, chlorophyll b and carotenoids were increased by 12.1%, 16.7% and 25.0%, respectively. Oil content was increased by 14.0% and reached to 25.7%, and the oleic acid content was significantly higher than that of the control and up to 16.4%. When co-cultured with Pseudomonas sp. (strain 2-1) for eight days at an initial ratio of 1:5, algal biomass, chlorophyll a, chlorophyll b and carotenoids contents were higher than that of the control by 47.9%, 16.0%, 17.5% and 19.9%, respectively. The total oil (27.1%) and oleic acid (18.2%) contents were increased by 20.4% and 64.0%, respectively. We concluded that Micrococcus (strain 1-2) and Pseudomonas sp. (strain 2-1) could significantly promote algal growth and increase oil production by their beneficial interaction with S. obliques, which could be potentially used in commercial production of S. obliques.

段露露, 程蔚兰, 张靖洁, 马浩天, 季春丽, 崔红利, 王计平, 李润植. 共生菌促进斜生栅藻生长和油脂合成[J]. 应用生态学报, 2020, 31(2): 625-633.

DUAN Lu-lu, CHEN Wei-lan, ZHANG Jing-jie, MA Hao-tian, JI Chun-li, CUI Hong-li, WANG Ji-ping, LI Run-zhi. Symbiotic bacteria facilitate algal growth and oil biosynthesis in Scenedesmus obliquus[J]. Chinese Journal of Applied Ecology, 2020, 31(2): 625-633.

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共生菌促进斜生栅藻生长和油脂合成

Symbiotic bacteria facilitate algal growth and oil biosynthesis in Scenedesmus obliquus

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