长柄木霉和泾阳链霉菌复配对烟苗生长及其抗病性的影响

应用生态学报

长柄木霉和泾阳链霉菌复配对烟苗生长及其抗病性的影响

张良^1,2,刘好宝^1**,顾金刚³,宋毓峰^1,2,董连红^1,2,苏玉龙^1,2,李世贵³,雷强⁴,伍仁军⁴,殷英⁴,屈健康⁴,王勇⁵

(¹农业部烟草生物学与加工重点实验室/中国农业科学院烟草研究所，山东青岛 266101； ²中国农业科学院研究生院，北京 100081； ^3中国农业科学院农业资源与农业区划研究所，北京 100081； ⁴四川省烟草专卖局，成都 610000； ^5四川省烟草公司凉山州公司，四川西昌 615000)

出版日期:2013-10-18 发布日期:2013-10-18

Effects of Trichoderma longbrachitum and Streptomyces jingyangensis combination on the growth and disease resistance of tobacco seedlings.

ZHANG Liang^1,2, LIU Hao-bao¹, GU Jin-gang³, SONG Yu-feng^1,2,DONG Lian-hong^1,2, SU Yu-long^1,2, LI Shi-gui³, LEI Qiang⁴, WU Ren-jun⁴, YIN Ying⁴, QU Jian-kang⁴, WANG Yong^5

(¹Ministry of Agriculture Key Laboratory of Tobacco Biology and Processing/Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China; ²Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; ³Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ⁴Sichuan Tobacco Monopoly Administration, Chengdu 610000, China; ⁵Liangshan Tobacco Co. Ltd., Xichang 615000, Sichuan, China)

Online:2013-10-18 Published:2013-10-18

摘要/Abstract

摘要： 以烟苗为试验材料，采用室内拮抗试验和活体筛选试验相结合的方法，研究了长柄木霉和泾阳链霉菌两种生防菌复配的亲和性和抑菌广谱性，并分析了生防菌及其组合对烟苗生长、诱导抗性和黑胫病防效的影响.结果表明：两生防菌株无相互抑制作用，具有较高的亲和性，并且两菌株本身及其代谢产物对不同生境下的病原菌(黑胫病、猝倒病和赤星病)均具有较高的抑菌活性和较广的抑菌谱.两菌株复配能影响根部和地上部的形态建成，促进烟苗根系和茎、叶的生长，显著增加地下部根系体积、根系总表面积、根长和根系平均直径以及地上部茎高、茎围、叶长、叶宽和生物量，具有显著的促生效能，并且两菌株复配的促生效应优于单独接种.生防菌组合能显著提高烟苗根系超氧化物歧化酶、过氧化物酶和过氧化氢酶等防御酶活性，对烟草黑胫病的相对防治效果达到了69.3%，具有显著的防病效能.研究表明长柄木霉和泾阳链霉菌是具有较高亲和性的增效组合，两菌株复配后在防病、促生方面能够发挥协同增效作用，可有效促进烟苗生长和防治烟草黑胫病.

Abstract:

An agar plate antagonism experiment in combining with in vivo screening experiment was conducted to study the affinity and bacteriostasis spectrum of the combination of biocontrol agents Trichoderma longbrachitum and Streptomyces jingyangensis to Nicotiana tabacum seedlings, with the effects of each agent and their combination on the N. tabacum seedlings growth, induced resistance, and resistance to Phytophthora nicotianae analyzed. The two agents had no interactive inhibitory effect and showed higher affinity to N. tabacum, and the agents themselves as well as their metabolites had higher bacteriostasis activities and wider bacteriostasis spectrum to P. nicotianae, Pythium aphanidermatum, and Alternaria alternate in different habitats. The combination of the two agents affected the morphological characteristics of the seedlings underground and aboveground parts, promoted the growth of root, stem, and leaf, and increased the root volume, total surface area, length, and average diameter as well as the stem height and size and the leaf length, width, and biomass, with these promotion effects being superior than those of the singleagent treatment. The combination of the two agents also increased the activities of the defensive enzymes superoxide dismutase, catalase, phenylalanine ammonia lyase, and peroxidase in the seedlings root significantly, with the relative control efficiency against P. nicotianae reached 69.3%, as compared to the conventional treatment. This study showed that the combination of T. longbrachitum and S. jingyangensis was a compatible combination with higher affinity and efficiency. This combination showed a synergistic effect of the two agents in plant disease control and in promoting plant growth, being able to promote the tobacco seedlings growth and control the P. nicotianae effectively.

张良1,2,刘好宝1**,顾金刚3,宋毓峰1,2,董连红1,2,苏玉龙1,2,李世贵3,雷强4,伍仁军4,殷英4,屈健康4,王勇5. 长柄木霉和泾阳链霉菌复配对烟苗生长及其抗病性的影响[J]. 应用生态学报.

ZHANG Liang1,2, LIU Hao-bao1, GU Jin-gang3, SONG Yu-feng1,2,DONG Lian-hong1,2, SU Yu-long1,2, LI Shi-gui3, LEI Qiang4, WU Ren-jun4, YIN Ying4, QU Jian-kang4, WANG Yong5. Effects of Trichoderma longbrachitum and Streptomyces jingyangensis combination on the growth and disease resistance of tobacco seedlings.[J]. Chinese Journal of Applied Ecology.