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纳米硒对氟胁迫下鲤鱼肝抗氧化功能及组织结构的影响

陈剑杰1,曹谨玲1**,罗永巨2,李巨银3   

  1. (1山西农业大学动物科技学院山西省生态畜牧与环境兽医学重点实验室, 山西太谷 030801; 2广西水产研究所, 南宁 530021; 3江苏农牧科技职业学院, 江苏泰州 225300)
  • 出版日期:2013-10-18 发布日期:2013-10-18

Effects of nano-selenium on antioxidant capacity and histopathology of Cyprinus carpio liver under fluoride stress.

CHEN Jian-jie1, CAO Jin-ling1, LUO Yong-ju2, LI Ju-yin3   

  1. (1Shanxi Province Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China; 2Guangxi Institute of Fisheries, Nanning 530021, China; 3Jiangsu Agrianimal Husbandry Vocational College, Taizhou 225300, Jiangsu, China)
  • Online:2013-10-18 Published:2013-10-18

摘要: 为探讨纳米硒(nano selenium, NSe)对氟暴露下鲤鱼肝脏的抗氧化功能和组织结构的保护作用,将750条鲤鱼随机分为5个组,分别为对照组(CK)、氟胁迫组(FS)和纳米硒低、中、高剂量组(NSe L、NSeM、NSeH),NSe L、NSeM、NSeH组在基础饵料中分别添加0.1、0.5、1.0 mg·kg-1的纳米硒,FS和 NSe组用100 mg·L-1氟溶液进行胁迫,试验时间为30 d.结果表明:与CK组比较,氟暴露降低了鲤鱼肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSHPx)的活性,增加了丙二醛(MDA)含量,肝脏的组织结构受到一定程度的损伤;与FS组比较,NSe增强了鲤鱼肝组织中SOD、CAT和GSH-Px活性,降低了MDA含量,缓解了氟胁迫对鲤鱼肝脏组织结构的损伤.表明饵料中添加纳米硒可在一定程度上改善氟胁迫所造成的鲤鱼抗氧化能力的下降和组织结构的损伤.

Abstract: To evaluate the protection effect of nanoselenium (NSe) on the antioxidant capacity and histopathology of Cyprinus carpio liver under fluoride stress, a total of 750 C. carpio individuals were randomly divided into five groups, i.e., no fluoride stress and NSe addition (CK), fluoride (100 mg F-·L-1)stressed (FS), and fluoridestressed plus NSe added with a dosage of 0.1 mg Se·L-1 (NSe L), 0.5 mg Se·L-1 (NSe M), and 1.0 mg Se·kg-1 (NSe H). The NSe was mixed with fish foods, and the fishes of FS and NSe groups were exposed to the fluoride stress for 30 days. As compared with CK, fluoride stress decreased the SOD, CAT, and GSHPx activities and increased the MDA content of C. carpio liver, and induced a definite damage on the histopathology of the liver. Compared with FS, NSe increased the liver SOD, CAT, and GSH-Px activities, decreased the liver MDA content, and mitigated the damage of fluoride stress on the histopathology of the liver. The results demonstrated that in some extent, the addition of NSe into fish foods could alleviate the decline of the antioxidant capacity of C. carpio liver and the damage on the liver histopathology caused by fluoride stress.