不同生态条件下水稻籼粳交后代亚种分化机制

应用生态学报

不同生态条件下水稻籼粳交后代亚种分化机制

王鹤潼^1，2,金峰¹,江奕君³,林青山⁴,徐海¹,陈温福¹,徐正进^1**

(¹沈阳农业大学水稻研究所农业部东北水稻生物学与遗传育种重点实验室/教育部北方粳稻育种重点实验室, 沈阳 110866; ^2中国科学院沈阳应用生态研究所，沈阳 110016；
³广东省农业科学院水稻研究所, 广州 510640； ^4广东省农业技术推广总站, 广州 510500)

出版日期:2013-11-18 发布日期:2013-11-18

Mechanisms of subspecies differentiation in a filial generation of rice indica-japonica hybridization under different ecological conditions.

WANG He-tong^1，2, JIN Feng¹, JIANG Yi-jun³, LIN Qing-shan⁴, XU Hai¹, CHEN Wen-fu¹, XU Zheng-jin^1

(¹Ministry of Agriculture Key Laboratory of Northeast Rice Biology and Breeding/Ministry of Education Key Laboratory of Northern Japonica Rice Genetics and Breeding, Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China; ²Institute of Applied Ecology, Chinese Academy of Sciences， Shenyang 110016, China;
³Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; ^4Guangdong Province General Startion for Crop Technology Extension, Guangzhou 510500, China)

Online:2013-11-18 Published:2013-11-18

摘要/Abstract

摘要：

籼粳交育种已成为国内最重要的育种方法之一,明确籼粳亚种分化机制是指导籼粳交育种的关键.本文用InDel(插入/缺失)和ILP(内含子长度多态性)标记,对在辽宁省和广东省种植并用单粒传法、混合法及系谱法构建的籼粳交(七山占/秋光)F₆代群体进行分析.结果表明: 混合法与单粒传法群体无偏分离,粳型判别值(Dj)集中在40%～60%之间；两地系谱法群体总体偏籼(30%～55%),且广东群体(38%)比辽宁(42%)偏籼,两地群体分布呈显著差异.混合法与单粒传法群体中Dj与重要农艺性状基因区域血缘呈显著正相关；而在系谱法中部分正相关被打破,且两地群体籼粳血缘呈规律性分布.表明人工选择是影响籼粳分化的主要因素,并与自然选择协同作用引起群体偏分离现象；群体中一些重要农艺性状的变化与籼粳分化间存在紧密的关系,这可能是影响籼粳交育种在综合亚种优势上未达到预期效果的关键.

Abstract: Indica-japonica hybridization is one of the most important breeding methods in China, whereas identifying subspecies differentiation mechanisms is the key in indica-japonica hybridization breeding. By using InDels (Insert/Deletion) and ILPs (Intron Length Polymorphism), an analysis was made on the F₆ populations derived from the hybridization of indica-japonica (Qishanzhan/Akihikari) planted in Liaoning and Guangdong provinces and generated by bulk harvesting (BM), singleseed descent methods (SSD), and pedigree method (PM). No segregation distortion was observed for the BM and SSD populations. The frequency distribution of japonica kinship percentage (Dj) was concentrated in 40%-60%. The PM populations in the two provinces presented indicadeviated distribution (30%-55%), with significant difference between Guangdong (38%) and Liaoning (42%). In addition, there was a significant positive correlation between the Dj and the kinship of functional gene regions in the BM and SSD populations. However, part of the positive correlation was broken in the PM populations that showed a regular distribution in the genotype patterns of indica and japonica loci. The above results demonstrated that artificial selection could be the main factor affecting the population differentiation in indica-japonica hybridization, and, with the synergistic effect of natural selection, induced the phenomenon of segregation distortion. There existed a close relationship between the differentiation of subspecies and the important agronomic traits, which could be the main reason why indica-japonica hybridiation breeding could not achieve the expected effect of combining the two subspecies advantages.

王鹤潼1，2,金峰1,江奕君3,林青山4,徐海1,陈温福1,徐正进1**. 不同生态条件下水稻籼粳交后代亚种分化机制[J]. 应用生态学报.

WANG He-tong1，2, JIN Feng1, JIANG Yi-jun3, LIN Qing-shan4, XU Hai1, CHEN Wen-fu1, XU Zheng-jin1. Mechanisms of subspecies differentiation in a filial generation of rice indica-japonica hybridization under different ecological conditions.[J]. Chinese Journal of Applied Ecology.