山东省二点委夜蛾不同地理种群遗传结构

应用生态学报

山东省二点委夜蛾不同地理种群遗传结构

王静¹,于毅¹,陶云荔²,李丽莉^1**,褚栋²

(¹山东省农业科学院植物保护研究所山东省植物病毒学重点实验室，济南 250100； ²青岛农业大学农学与植物保护学院山东省植物病虫害综合防控重点实验室，山东青岛 266109)

出版日期:2014-02-18 发布日期:2014-02-18

Genetic structure of the geographical populations of Athetis lepigone in Shandong Province, China.

WANG Jing¹, YU Yi¹, TAO Yun-li², LI Li-li¹, CHU Dong^2

(¹Key Laboratory for Plant Virology of Shandong, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China; ²Shandong Province Key Laboratory of Integrated Crop Pest Management, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, Shandong, China)

Online:2014-02-18 Published:2014-02-18

摘要/Abstract

摘要：

二点委夜蛾是近年来在我国黄淮海夏玉米区暴发为害的一种玉米新害虫.为了系统揭示二点委夜蛾种群在山东省的扩散途径与发生规律，本研究利用线粒体细胞色素氧化酶亚基I(mtCOI)基因对山东省12个地市的二点委夜蛾种群以及威海1个二点委夜蛾形态近似种种群进行遗传结构分析.结果表明： 130条二点委夜蛾mtCOI基因(608 bp)共有24个单倍型，7条二点委夜蛾形态近似种的mtCOI基因共有2个单倍型.单倍型网络图与系统发育树显示，威海地区二点委夜蛾形态近似种与其他种群分为2大组，它们之间存在着显著遗传分化，遗传距离为0.044～0.054.AMOVA分析表明，山东省二点委夜蛾种群遗传变异主要来自组间，该地区二点委夜蛾并没有经历过种群扩张.该研究为此害虫的预测预报及防治提供了科学依据.

Abstract: Athetis lepigone (M-schler) is a new insect pest of summer corn in HuangHuaiHai Region of China. To understand the spread and damage of this insect pest in Shandong Province, we analyzed the genetic structure of the A. lepigone populations collected from 12 counties and one morphologically alike species from Weihai using mitochondrial cytochrome oxidase I (mtCOI, 608 bp) gene. The results showed that there were 24 mtCOI haplotypes generated from 130 sequences of A. lepigone and 2 haplotypes from 7 sequences of morphologically alike species of A. lepigone. The haplotype network and phylogenetic tree showed that the populations in the study formed two distinct groups, i.e., the population of Weihai (WH) and the other populations. The two groups were significantly differentiated and the genetic distance between them was 0.044-0.054. Molecular variance (AMOVA) analysis showed that a high proportion of the total genetic variance was attributed to variations among groups, indicating that there was no obvious population expansion among A. lepigone populations in this province. The study provided the scientific basis for the forecasting and prevention of the insect pest.

王静1,于毅1,陶云荔2,李丽莉1**,褚栋2. 山东省二点委夜蛾不同地理种群遗传结构[J]. 应用生态学报.

WANG Jing1, YU Yi1, TAO Yun-li2, LI Li-li1, CHU Dong2. Genetic structure of the geographical populations of Athetis lepigone in Shandong Province, China.[J]. Chinese Journal of Applied Ecology.