胜利油田采油区土壤石油污染状况及其微生物群落结构

应用生态学报

胜利油田采油区土壤石油污染状况及其微生物群落结构

刘健^1,2,宋雪英³,孙瑞莲^1,2**,解伏菊⁴,王仁卿^1,2,王文兴¹

(¹山东大学环境研究院, 济南 250100; ²山东大学生态学与生物多样性研究所, 济南 250100; ^3沈阳大学区域污染环境生态修复教育部重点实验室, 沈阳 110044; ⁴济南大学资源与环境学院, 济南 250022)

出版日期:2014-03-18 发布日期:2014-03-18

Petroleum pollution and the microbial community structure in the soil of Shengli Oilfield.

LIU Jian^1，2, SONG Xue-ying³, SUN Rui-lian^1,2, XIE Fu-ju⁴, WANG Ren-qing^1,2, WANG Wen-xing^1

(¹Environment Research Institute, Shandong University, Jinan 250100, China; ^2Institute of Ecology and Biodiversity, Shandong University, Jinan 250100, China; ³Key Laboratory of Regional Environment and EcoRemediation, Ministry of Education， Shenyang University, Shenyang 110044, China; ⁴School of Resources and Environment, University of Jinan, Jinan 250022, China)

Online:2014-03-18 Published:2014-03-18

摘要/Abstract

摘要：

基于不同开采年代新油井(2011—)和老油井(1966—2003年)周边土壤的调查取样,研究了采油区土壤石油污染状况,利用PCR-DGGE和克隆测序技术,探讨了新、老油井周边土壤微生物的群落结构.结果表明:油井周边土壤均受到不同程度的石油污染,其石油烃含量大多高于土壤石油污染临界值(500 mg·kg^-1),且老油井周边土壤污染水平更高.污染土壤石油烃含量与土壤有机碳、全氮和速效钾含量呈显著正相关.老油井周边土壤微生物群落多样性指数随污染水平的增大而减小,新油井则呈相反的趋势.DGGE图谱优势条带测序结果表明,油井周边土壤均存在明显的优势菌,大多为石油烃相关菌和烃类降解菌,如微杆菌属、链霉菌属、迪茨氏菌属、黄杆菌属及α、γ变形菌等.

Abstract: Soils around a new oil well (2011- ) and an old oil well (1966-2003) were sampled to investigate the characteristics of petroleum pollution in the oilfield. The structure of soil microbial community was analyzed by PCRDGGE and clone sequencing techniques. Results showed that the soils around the two oil wells were generally contaminated with petroleum, and the concentrations of total petroleum hydrocarbons mostly exceeded the threshold of the environmental quality standards of soil (500 mg·kg^-1). The total petroleum hydrocarbons concentration of the polluted soil was significantly positively correlated with the contents of organic carbon, total nitrogen and available potassium, respectively. The microbial diversity index in the soil around the old oil well decreased with the increasing total petroleum hydrocarbons concentration, however, it was reversed for the soil around the new oil well. Sequence analysis of the prominent bands in DGGE profiles showed that some dominant species existed in the petroleumcontaminated soils around the oil wells and mostly were oilassociated and hydrocarbon degrading bacteria, including Microbacterium, Streptomyces, Dietzia, Flavobacterium, αProteobacteria, and γProteobacteria.

刘健1,2,宋雪英3,孙瑞莲1,2**,解伏菊4,王仁卿1,2,王文兴1. 胜利油田采油区土壤石油污染状况及其微生物群落结构[J]. 应用生态学报.

LIU Jian1，2, SONG Xue-ying3, SUN Rui-lian1,2, XIE Fu-ju4, WANG Ren-qing1,2, WANG Wen-xing1. Petroleum pollution and the microbial community structure in the soil of Shengli Oilfield.[J]. Chinese Journal of Applied Ecology.