短期落干对水稻土反硝化微生物丰度和N2O释放的影响

应用生态学报

短期落干对水稻土反硝化微生物丰度和N₂O释放的影响

卢静^1,2,3,刘金波⁴,盛荣^1,2,刘毅^1,2,陈安磊^1,2,魏文学^1,2**

(¹中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室，长沙 410125； ²桃源农业生态试验站，长沙 410125； ³中国科学院大学，北京 100049; ^4中国科学院青藏高原研究所, 北京 100101)

出版日期:2014-10-18 发布日期:2014-10-18

Effect of shorttime drought process on denitrifying bacteria abundance and N₂O emission in paddy soil.

LU Jing^1,2,3, LIU Jin-bo⁴, SHENG Rong^1,2, LIU Yi^1,2, CHEN An-lei^1,2, WEI Wen-xue^1,2

(¹Key Laboratory of Agroecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;²Taoyuan Station of Agroecology Research, Changsha 410125, China; ³University of Chinese Academy of Sciences, Beijing 100049, China； ⁴Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China)
.

Online:2014-10-18 Published:2014-10-18

摘要/Abstract

摘要：

为了探明水稻土落干过程对温室气体排放和反硝化微生物的影响，通过模拟水稻土淹水落干过程，系统监测了落干开始后24 h内N2O的释放和氧化还原电位(Eh)的变化，并利用实时PCR(qPCR)方法测定了反硝化功能基因narG和nosZ的丰度.结果表明：落干开始后4 h N₂O释放量就明显增加，在24 h时N₂O的释放量比淹水对照增加了5倍多；narG和nosZ基因丰度也随着落干过程的推移而快速增加；而且N₂O排放通量与narG基因呈极显著相关(P<0.01).表明水稻土短期淹水落干过程中，含narG基因反硝化微生物是驱动N₂O释放的主要功能微生物.

Abstract: In order to investigate the impact of drying process on greenhouse gas emissions and denitrifying microorganisms in paddy soil, wettingdrying process was simulated in laboratory conditions. N₂O flux, redox potential (Eh) were monitored and narG and nosZcontaining denitrifiers abundances were determined by realtime PCR. N₂O emission was significantly increased only 4 h after drying process began, and it was more than 6 times of continuous flooding (CF) at 24 h. In addition, narG and nosZ gene abundances were increased rapidly with the drying process, and N₂O emission flux was significantly correlated with narG gene abundance (P<0.01). Our results indicated that the narGcontaining deniteifiers were the main driving microorganisms which caused the N₂O emission in the short-time drought process in paddy soil.

卢静1,2,3,刘金波4,盛荣1,2,刘毅1,2,陈安磊1,2,魏文学1,2**. 短期落干对水稻土反硝化微生物丰度和N₂O释放的影响[J]. 应用生态学报.

LU Jing1,2,3, LIU Jin-bo4, SHENG Rong1,2, LIU Yi1,2, CHEN An-lei1,2, WEI Wen-xue1,2. Effect of shorttime drought process on denitrifying bacteria abundance and N₂O emission in paddy soil.[J]. Chinese Journal of Applied Ecology.

短期落干对水稻土反硝化微生物丰度和N₂O释放的影响

Effect of shorttime drought process on denitrifying bacteria abundance and N₂O emission in paddy soil.

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