不同浓度铜对紫背萍和青萍色素含量及抗氧化酶系统的影响

应用生态学报

不同浓度铜对紫背萍和青萍色素含量及抗氧化酶系统的影响

涂俊芳;王兴明;刘登义;李晶;李征

安徽师范大学生物多样性研究中心，重要生物资源保护与利用安徽省重点试验室，芜湖 241000

收稿日期:2005-06-03 修回日期:2005-11-08 出版日期:2006-03-18 发布日期:2006-03-18

Effects of different concentration copper on pigment content and antioxidase system of Spirodela polyrrhiza and Lemna minor

TU Junfang;WANG Xingming;LIU Dengyi;LI Jing;LI Zheng

Provincial Key Laboratory of Conservation and Exploitation of Biological Resources in Anhui,Biodiversity Research Center,Anhui Normal University,Wuhu 241000,China

Received:2005-06-03 Revised:2005-11-08 Online:2006-03-18 Published:2006-03-18

摘要/Abstract

摘要： 通过水培试验研究了不同浓度的重金属Cu对紫背萍(Spirodela polyrrhiza)和青萍(Lemna minor)的色素含量和抗氧化酶系统的影响.结果表明,低浓度Cu(0.056 mg·L^-1)的处理下，紫背萍和青萍的叶绿素a、叶绿素b和叶绿素(a+b)的含量均出现不同程度的增加，分别高出其对照11％、46％、22％和8％、15％、11％，而在高浓度Cu(0.18～5.60 mg·L^-1)的处理下，上述色素含量均显著下降，平均下降幅度分别达63％、62％、65%和46％、44％、45％.紫背萍体内丙二醛(MDA)含量为青萍的2.57倍.两种浮萍的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性均随Cu浓度的增加而呈先升后降的趋势，紫背萍体内3种酶活性在Cu浓度仅为0.18 mg·L^-1时即达峰值，随后则大幅下降，而青萍体内3种酶活性却在Cu浓度分别升高到0.56、1.0和1.0 mg·L^-1时才达到峰值.可见，在Cu胁迫下，紫背萍受Cu毒害较青萍深，且其体内抗氧化酶系统也较青萍敏感.

关键词: 沼泽湿地, 植物器官, N素动态变化

Abstract: The study with water culture showed that when treated with 0.056 mg Cu·L^-1,the chlorophyll a,b and (a+b) contents in Spirodela polyrrhiza and Lemna minor were increased by 11%,46% and 22%,and 8%,15% and 11%,while under 0.15～5.6 mg Cu·L^-1,the contents were decreased by 63%,62% and 63%,and 46%,45% and 45%,respectively,compared with no copper added.The malondialdehyde (MDA) content in S.polyrrhiza was 2.75 times higher than that in L.minor averagely.The superoxide dismutase (SOD),catalase (CAT) and peroxidase (POD) activities in both S.polyrrhiza and L.minor all increased first,but decreased then with increasing copper concentration.For S.polyrrhiza,the activities of test enzymes were the highest at 0.18 mg Cu·L^-1,but for L.minor,only when the Cu concentrations were up to 0.56,1.0 and 1.0 mg·L^-1,the activities of SOD,CAT and POD got to their peak values,respectively.It was concluded that higher concentrations of copper caused more damage to S.polyrrhiza than to L.minor,and the antioxidase system of the former was much more sensitive to the copper stress than that of the latter.

Key words: Swamp wetland, Vegetable organs, N content dynamics

涂俊芳;王兴明;刘登义;李晶;李征. 不同浓度铜对紫背萍和青萍色素含量及抗氧化酶系统的影响[J]. 应用生态学报.

TU Junfang;WANG Xingming;LIU Dengyi;LI Jing;LI Zheng. Effects of different concentration copper on pigment content and antioxidase system of Spirodela polyrrhiza and Lemna minor[J]. Chinese Journal of Applied Ecology.

[1]	赵旭, 鲁绍伟, 李少宁, 徐晓天, 赵娜. 暖温带典型树种春季物候期水溶性化合物δ¹³C变化规律 [J]. 应用生态学报, 2022, 33(6): 1466-1474.
[2]	曾素平, 刘发林, 赵梅芳, 艾瑶, 陈小伟. 我国亚热带典型树种抗火性状随林龄和器官的变异 [J]. 应用生态学报, 2020, 31(4): 1063-1072.
[3]	沈超, 纪若璇, 于笑, 白雪卡, 常远, 刘超. 蒙古莸幼苗干旱致死过程中非结构性碳水化合物的变化 [J]. 应用生态学报, 2019, 30(8): 2541-2548.
[4]	王凯, 沈潮, 孙冰, 王潇楠, 魏东, 吕林有. 干旱胁迫对科尔沁沙地榆树幼苗C、N、P化学计量特征的影响 [J]. 应用生态学报, 2018, 29(7): 2286-2294.
[5]	王凯, 沈潮, 曹鹏, 宋立宁, 于国庆. 沙地樟子松幼苗干旱致死过程中非结构性碳水化合物的变化 [J]. 应用生态学报, 2018, 29(11): 3513-3520.
[6]	刘德燕^1，2;宋长春¹. 湿地植物小叶章对外源氮输入的响应 [J]. 应用生态学报, 2008, 19(12): 2599-2604 .
[7]	邢福^1,2;吕宪国¹;倪红伟³;高玉慧³;许坤²;于丽丽²;张亮². 三江平原沼泽湿地群落演替系列β多样性 [J]. 应用生态学报, 2008, 19(11): 2455-2459 .
[8]	孙丽^1,2;宋长春¹. 三江平原典型沼泽湿地蒸散发估测 [J]. 应用生态学报, 2008, 19(09): 1925-1930 .
[9]	王德宣;宋长春;王毅勇;赵志春. 若尔盖高原沼泽湿地与草地二氧化碳通量的比较 [J]. 应用生态学报, 2008, 19(02): 285-289 .
[10]	徐治国^1,2;何岩³;闫百兴¹;宋长春¹. 三江平原典型沼泽湿地植物种群的生态位 [J]. 应用生态学报, 2007, 18(04): 783-787 .
[11]	宋长春^1,2;王毅勇¹. 湿地生态系统土壤温度对气温的响应特征及对CO₂排放的影响 [J]. 应用生态学报, 2006, 17(04): 625-629 .
[12]	王毅勇^1,2;郑循华²;宋长春¹;赵志春¹. 三江平原典型沼泽湿地氧化亚氮通量 [J]. 应用生态学报, 2006, 17(03): 493-497 .
[13]	宋长春, 王毅勇, 阎百兴, 张金波, 娄彦景. 沼泽湿地垦殖前后土壤温度变化及其对土壤热状况的影响 [J]. 应用生态学报, 2005, 16(1): 88-92.
[14]	宋长春^1,2；王毅勇¹；阎百兴¹；张金波¹；娄彦景¹. 沼泽湿地垦殖前后土壤温度变化及其对土壤热状况的影响 [J]. 应用生态学报, 2005, 16(01): 88-92 .
[15]	刘振乾, 王建武, 骆世明, 吕宪国, 刘兆礼, 刘红玉, 李秀军. 基于水生态因子的沼泽安全阈值研究——以三江平原沼泽为例 [J]. 应用生态学报, 2002, (12): 1610-1614.