转Bt基因及其亲本水稻上褐飞虱对三唑磷和溴氰菊酯的反应

应用生态学报

转Bt基因及其亲本水稻上褐飞虱对三唑磷和溴氰菊酯的反应

李向冬^1,2,杨亚军¹,田俊策¹,徐红星¹,郑许松¹,吕仲贤^1,2**

(¹浙江省农业科学院植物保护与微生物研究所浙江省植物有害生物防控省部共建国家重点实验室培育基地，杭州 310021; ^2南京农业大学植物保护学院，南京 210095)

出版日期:2014-10-18 发布日期:2014-10-18

Ecological responses of brown planthopper, Nilaparvata lugens, to triazophos and deltamethrin on transgenic Bt rice lines and their nonBt parental rice.

LI Xiang-dong^1,2, YANG Ya-jun¹, TIAN Jun-ce¹, XU Hong-xing¹, ZHENG Xu-song¹, LU Zhong-xian^1，2

(¹State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ²College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China)

Online:2014-10-18 Published:2014-10-18

摘要/Abstract

摘要：

以转Bt基因抗虫水稻T1C-19(含cry1C基因)和T2A-1(含cry2A基因)及其亲本水稻MH63为材料，用20和40 mg·L^-1的三唑磷以及1、3和6 mg·L^-1的溴氰菊酯喷雾分别处理稻株上的3龄褐飞虱若虫，研究了两种农药对转Bt基因抗虫水稻上褐飞虱再猖獗的影响.结果表明：三唑磷处理对褐飞虱的若虫历期无显著性影响，溴氰菊酯则能显著降低若虫历期，但随着两种药剂处理浓度的升高，若虫的存活率降低、成虫的产卵量增加.在同一浓度农药药剂处理下，3个水稻品种上的褐飞虱若虫发育历期、若虫存活率、初羽雌成虫体质量、产卵量和卵孵化率等生态学参数均没有显著差异.表明褐飞虱在两种转Bt水稻上对三唑磷和溴氰菊酯诱导再猖獗的反应能力与其亲本水稻MH63没有差异.

Abstract: In this study, the brown planthopper (BPH), Nilaparvata lugens (Hemiptera: Delphacidae), cultured on Bt rice lines (T1C-19 with cry1C gene and T2A-1 with cry2A gene) and their parental rice MH63, were treated with a series of concentrations of triazophos and deltamethrin when they developed up to the third instar for the evaluation of the effects of Bt rice on BPH resurgence induced by pesticides. The results indicated that BPH nymphal duration decreased with the increasing concentration of deltamethrin, while no significant differences of BPH nymphal duration were observed on the same rice variety treated with the different concentrations of triazophos. Furthermore, the survival rate of BPH significantly declined and their fecundity significantly increased with the increasing concentration of insecticides. However, ecological fitness parameters of BPH including nymphal duration, survival rate, female adult mass, fecundity and egg hatchability were not significantly different among Bt and nonBt rice varieties when they treated with the same concentration of insecticide. These results implied that no significant difference in the BPH resurgence induced by pesticides was observed among the two tested Bt rice varieties and their nonBt parental rice variety.

李向冬1,2,杨亚军1,田俊策1,徐红星1,郑许松1,吕仲贤1,2**. 转Bt基因及其亲本水稻上褐飞虱对三唑磷和溴氰菊酯的反应[J]. 应用生态学报.

LI Xiang-dong1,2, YANG Ya-jun1, TIAN Jun-ce1, XU Hong-xing1, ZHENG Xu-song1, LU Zhong-xian1，2. Ecological responses of brown planthopper, Nilaparvata lugens, to triazophos and deltamethrin on transgenic Bt rice lines and their nonBt parental rice.[J]. Chinese Journal of Applied Ecology.