Corn seedlings are often harmed by strong wind-sand in the spring in semiarid windsand area of west of Northeast China. In order to understand physiological response mechanisms of the corn seedlings to windsand damage, the changes in MDA content, membrane permeability, protective enzymes activities and osmotic regulation substances at 0 (CK), 6, 9, 12, 15 and 18 m·s^-1 wind speed (wind-sand flow strength: 0, 1.00, 28.30, 63.28, 111.82 and 172.93 g·cm^-1·min^-1, respectively) for 10 min duration were studied during the spring, 2013 in the Horqin Sand Land of Inner Mongolia. The results showed that effects of windsand flow blowing on the RWC of the corn seedling were lighter in the 6-12 m·s^-1 treatments, but the RWC decreased by 19.0% and 18.7% in the 15 m·s^-1 and 18 m·s^-1 treatments compared to the CK, respectively. The MDA content tended to decline with increasing the wind-sand flow strength, and decreased by 35.0% and 39.0% in the 15 m·s^-1 and 18 m·s^-1 treatments compared to the CK, respectively. The membrane permeability increased significantly with increasing the windsand flow strength, and increased by 191.3% and 187.8% in the 15 m·s^-1 and 18 m·s^-1 treatments compared to the CK, respectively. With the increase of windsand flow strength, SOD activities decreased and changes of CAT activities were not significant, only POD activities increased significantly, which played an important role in the process of scavenging reactive oxygen species and protecting cell membrane against damage. For lighter water stress caused by windsand flow blowing, proline and soluble sugar did not play any role in osmotic adjustment, but the proline content increased by 11.4% and 24.5% in the 15 m·s^-1 and 18 m·s^-1 treatments compared to the CK, respectively, which played an important role in osmotic adjustment.