In this study, Xianyu 335, a maize hybrid, was used to investigate the effects of 24Epibrassinolide (EBR, a synthetic BR) on antioxidant capacity and lowtemperature response gene expression in maize embryo germination under low temperature (LT) stress. The germination rate of maize seeds under LT stress was not affected by EBR, but the seed activity index and seedling growth were improved. EBR increased the activities of some antioxidative enzymes including SOD, POD, CAT and GR, and the contents of nonenzymatic antioxidants, such as GSH and proline, and induced the accumulation of nitric oxide (NO). NO scavenging cPTIO and NOS inhibitor LNAME decreased but NO donor SNP increased the enzyme activities of CAT and POD, and the content of proline, indicating NO mediated the EBRinduced antioxidant capacity. The gene expression pattern analysis showed that the expression of P₅-CS₁, CBF₁, CBF₃ and COR₁₅a was induced by LT stress, and further increased by EBR treatment in maize embryo, while their expression was suppressed by cPTIO and L-NAME, and improved by SNP, which implied LTresponsed genes were regulated by NO. These results demonstrated that NO was involved in the EBRinduced LT tolerance in maize embryo by modulating the antioxidative capacity and the expression of LTresponsive genes.