Abstract: Taking ‘Miaoxiang No.7’ strawberry as material, full red light, full blue light, full yellow light, full white light, red/blue/yellow (7/2/1), red/blue (7/2) light generated by light emitting diode (LED) was applied to accurately modulate with white light generated as control. The indicators of photosynthetic and fluorescence parameters, pigment content, fruit production and quality, root activity were investigated. The effects of light quality under the light intensity (500 μmol·m^-2·s^-1) on the photosynthetic characteristic, fruit production and quality of strawberry were studied. The results showed that the red light could increase photosynthetic parameters (P_n, T_r), while blue light had inhibitory effect. Intercellular CO₂ concentration (C_i) and conductance (g_s) were the highest under blue light. The fluorescence parameters were significantly affected by light quality, F_o, F_m and Φ_PSⅡ the highest under red light, but values of the maximal photochemical of PSⅡ (F_v/F_m), F_v/F_o and F_m/F_o highest under red/blue/yellow (7/2/1). In addition, the soluble solids content and vitamin C were highest under red light, the blue light could increase protein and titratable acid, sugaracid ratio was the highest under red/blue/yellow (7/2/1). Comprehensive analysis indicated that red/blue/yellow (7/2/1) was more beneficial to the increase of pigment contents of leaves, fruit production and some qualities of strawberry.