Abstract: Topsoil of green space including typical forest, shrub and grassland were collected to measure their water soluble organic carbon (WSOC) before and after incubation of 30 days at 5, 15, 25, 35 and, 45 ℃. The results showed the average values of WSOC were higher in urban than in rural green spaces, but the percentage of WSOC to total organic carbon (TOC) showed an opposite trend. No significant changes were found among the three green space types in WSOC and WSOC/TOC. Response of WSOC in green space to incubation temperature was generally highest in urban sites, followed by suburban sites, and lowest in rural sites at the incubation temperature of 5 ℃, but showed an opposite trend at the temperature of 45 ℃. Response coefficient of WSOC to temperature change was lower in forest and shrub than in grassland, but increased along the urbanrural gradient. Further analysis showed that WSOC positively correlated with TOC, total nitrogen and available phosphorus, and the response coefficient of WSOC to temperature change negatively correlated with available phosphorus. In summary, exogenous substances input might lead to the accumulation of WSOC in urban green space, however, urban environment was helpful to maintain the stability of WSOC, which might be due to the enrichment of available phosphorus in urban sites.